Process of preparing alkali metal salts of hydrophobic free sulfonamides

专利摘要:
Thienyl-, furyl- and pyrrolyl-sulfonamides, pharmaceutically acceptable salts of sulfonamides, formulations and sulfonamides of salts, and the formulations and sulfonamides used to modulate or alter the activity of the endothelin family of peptides. Provide a method. In particular, formulations of sodium salts of N- (isoxazolyl) thienylsulfonamide, N- (isoxazolyl) furylsulfonamide and N- (isoxazolyl) pyrrosulfonamide are provided. Provided are methods for preparing alkali metal salts of hydrophobic sulfonamides. The method comprises dissolving the free sulfonamide in an organic solvent in the presence of a saturated alkali metal salt solution and recovering the formed sulfonamide salt from the organic phase.
公开号:KR20040004368A
申请号:KR10-2003-7002757
申请日:1998-04-02
公开日:2004-01-13
发明作者:우쳉데；블록나탈리；코간티모씨；켈러케런；우다드패트리샤
申请人:텍사스 바이오테크놀로지 코포레이션；
IPC主号:

专利说明:

Process of preparing alkali metal salts of hydrophobic free sulfonamides
[1] Related patents
[2] For purposes of the US national stage, the present application is directed to US Patent Application No. 08 / 938,444 to Blok et al., Filed Sep. 27, 1997, entitled “Formulation of Sulfonamide for Treatment of Endothelin-Mediated Diseases”. Partial continuous application. This application is also a partial consecutive application of US Patent Application Serial No. 08 / 847,797 to Block et al., Filed April 28, 1997, entitled "Method for Preparing Alkali Metal Salts of Hydrophobic Sulfonamide". For international purposes, the two patent applications cited above are claimed as priority.
[3] This application is related to U.S. Patent Application Serial No. 08 / 938,325, filed Sep. 27, 1997, entitled “Sulfonamides and Derivatives That Modulate the Activity of Endothelin,” and Sulfonamides and derivatives thereof that modulate the activity of endothelin "are related to WOT et al., PCT / US97 / 17402, filed Sep. 27, 1997 in the name of the invention. This application is related to US Patent Application No. 08 / 721,183 to Chan et al., Filed Sep. 27, 1996, entitled “Sulfonamides and Derivatives That Modulate the Activity of Endothelin”; Also referred to as " thienyl-, furyl-, pyrrolyl- and biphenylsulfonamide and derivatives thereof that modulate the activity of endothelin, " Related to US96 / 04759; US Patent Application No. 08 / 477,223 to Chan et al., Filed Jun. 6, 1995, entitled " thienyl-, furyl- and pyrrolylsulfonamide and derivatives thereof that modulate the activity of endothelin, " Currently associated with US Pat. No. 5,594,021; Also referred to as "thienyl-, furyl- and pyrrolylsulfonamide and derivatives thereof that modulate the activity of endothelin" was filed on April 4, 1995 and is now abandoned by US Patent Application Serial No. Related to 08 / 417,075; "Sulfonamides and derivatives thereof that modulate the activity of endothelin" is related to U.S. Patent Application Serial No. 08 / 247,072, filed May 20, 1994, and now U.S. Patent No. 5,571,821, filed May 20, 1994; US Patent Application No. 08 / 222,287 to Chan et al., Filed Apr. 5, 1994, entitled “thiophenyl-, furyl- and pyrrolylsulfonamides and derivatives thereof that modulate the activity of endothelin,” Currently associated with US Pat. No. 5,591,761; Each of the above patent applications is filed on Oct. 21, 1993, in the United States of the United States, under the name "N- (4-halo-isoxazolyl) -sulfonamide and derivatives thereof that modulate the activity of endothelin." Patent application 08 / 142,552, currently US Pat. No. 5,514,691; October 21, 1993, entitled "N- (5-isoxazolyl) biphenylsulfonamide, N- (3-isoxazolyl) biphenylsulfonamide and derivatives thereof that modulate endothelin activity" U.S. Patent Application Serial No. 08 / 142,159 to Chan et al., US Patent No. 5,464,853; And "N- (5-isoxazolyl) -benzenesulfonamide, N- (3-isoxazolyl) -benzenesulfonamide and derivatives thereof that modulate the activity of endothelin," October 21, 1993. No. 08 / 142,631 to Chan et al., Filed and abandoned at present.
[4] Where possible, the subject matter of each of the foregoing patent applications is hereby fully incorporated by reference.
[5] The present invention relates to compounds and formulations thereof for administration to mammals that modulate the activity of the endothelin family of peptides. In particular, formulations of sulfonamide and sulfonamide compounds for administration for the treatment of endothelin-mediated diseases, in particular the sodium salts of sulfonamide compounds, are provided. Also provided are methods of preparing alkali metal salts of hydrophobic sulfonamides.
[6] The vascular endothelium is endothelial-derived vasoconstrictor peptide, endothelin (ET) [see, for example, Vanhoutte et al. (1986) Annual Rev. Physiol. 48 : 307-320; Furchgott and Zawadski (1980) Nature 288 : 373-376]. Endothelin first identified in culture supernatants of porcine aortic endothelial cells [Yanagisawa et al. (1988) Nature 332 : 411-415] is a powerful 21 amino acid peptide vasoconstrictor. It is the most potent blood pressure enhancer known and is produced by many cell types including endothelial, organ, kidney and brain. Endothelin is synthesized as 203 amino acid precursor preproendothelin containing a signal sequence that is degraded by endogenous proteases to produce 38 (human) or 39 (pig) amino acid peptides. Said intermediate, called big endothelin, proceeds in mature biologically active form by putative endothelin-converting enzyme (ECE) which appears to be a metal-dependent neutral protease in vivo. See, eg, Kashiwabara et al. (1989) FEBS Lttrs. 247 : 337-340. Degradation is required for the induction of physiological responses. See, eg, von Geldern et al . (1991) Peptide Res. 4 : 32-35]. In porcine aortic endothelial cells, the 39 amino acid intermediate, Big Endothelin, is hydrolyzed at Trp ²¹ -Val ²² binding to produce endothelin-1- and C-terminal fragments. Similar degradation occurs in human cells from 38 amino acid intermediates. Three distinct isopeptides, endothelin-1, endothelin-2, and endothelin-3 have been identified that exhibit potent vasoconstrictor activity.
[7] The family of three isopeptides endothelin-1, endothelin-2 and endothelin-3 are encoded by three genes [Inoue et al. (1989) Proc. Natl. Acad. Sci. USA 86 : 2863-2867; Saida et al. (1989) J. Biol. Chem. 264 : 14613-14616. The nucleotide sequence of the three human genes is highly conserved within the region encoding the mature 21 amino acid peptide and the C-terminal portion of the peptide is identical. Endothelin-2 is (Trp ⁶ , Leu ⁷ ) endothelin-1- and endothelin-3 is (Thr ² , Phe ⁴ , Thr ⁵ , Tyr ⁶ , Lys ⁷ , Tyr ¹⁴ ) endothelin-1-. Thus, the peptide is highly conserved at the C-terminal end. Release of endothelin from cultured endothelial cells appears to be regulated by various chemical and physical stimuli and to levels of transcription and / or translation. The expression of genes encoding endothelin-1 is increased by chemical stimuli, including adrenaline, thrombin and Ca ²⁺ ion transport carriers. The production and release of endothelin from the endothelium is characterized by angiotensin II, vasopressin, endotoxin, cyclosporin and other factors [Brooks et al . (1991) Eur. J. Pharm. 194 : 115-117] and inhibited by nitric oxide. Endothelial cells are either nitric oxide or related substances when stimulated by vascular activators such as acetylcholine and Bradkinin [Palmer et al. (1987) Nature 327 : 524-526, including short-lived endothelial-induced relaxation factors (EDRF). Endothelin-induced vasoconstriction is also attenuated by atrial natriuretic peptide (ANP).
[8] Endothelin peptides exhibit a number of biological activities in vitro and in vivo. Endothelin causes strong and sustained vasoconstriction in rats in vivo and in isolated vascular smooth muscle preparations; It also causes the release of eicosanoids and endothelial-induced relaxation factors (EDRF) from the perfused blood vessel image. Intravenous administration of endothelin-1 and in vitro addition to blood vessels and other smooth muscle tissues cause long-term blood pressure enhancing and contraction, respectively . See, eg, Bolger et al. (1991) Can. J. Physiol. Pharmacol. 69 : 406-413. In isolated blood vessel strips, for example, endothelin-1 is potent (EC ₅₀ = 4 × 10 ⁻¹⁰ M), but slowly acting, but is a continuous contractile agent. In vivo, a single dose raises blood pressure within about 20-30 minutes. Endothelin-induced vasoconstriction is not affected by antagonists to known neurotransmitters or hormonal factors, but is negated by calcium channel antagonists. However, since calcium influx appears to be necessary for long-term contractile response to endothelin, the action of calcium channel antagonists seems to be almost the result of inhibition of potassium influx.
[9] In addition, endothelin mediates renin release in guinea pig aorta, stimulates ANP release and induces positive metabolic action. In the lungs, endothelin-1 acts as a potent bronchoconstrictor [Maggi et al. (1989) Eur. J. Pharmacol. 160 : 179-182]. Endothelin increases renal vascular resistance, reduces renal blood flow and decreases glomerular filtrate rate. It is an effective mitogen for renal glomerular vasculature cells and induces a phosphoinoside cascade in the cells . See Simonson et al. (1990) J. Clin. Invest. 85 : 790-797.
[10] There are specific high affinity binding sites (degradation constants in the range of 2-6 × 10 ⁻¹⁰ M) to endothelin in the vascular system and in other tissues including the intestine, heart, lung, kidney, spleen, adrenal gland and brain. Binding is not inhibited by catecholamines, vasoactive peptides, neurotoxins or calcium channel antagonists. Endothelin binds and interacts with receptors different from other autonomic nervous system receptors and voltage dependent calcium channels. Competitive binding studies suggest that there are many classes of receptors with different affinities for endothelin isopeptides. Sarapotoxin , a group of peptide toxins from the venom of the snake Atractaspis eingadensis , which causes severe coronary vasospasm in snake bites, is structural and functional for endothelin-1. Homozygous and competitively binds to the same heart membrane receptor [Kloog et al. (1989) Trends Pharmacol. Sci. 10 : 212-214].
[11] Two distinct endothelin receptors, named ET _A and ET _B , have been identified and DNA encoding each receptor has been isolated [Arai et al. (1990) Nature 348 : 730-732; Sakurai et al. (1990) Nature 348 : 732-735. Based on the amino acid sequence of the protein encoded by the cloned DNA, each receptor appears to contain seven membrane spanning domains and exhibit structural similarity to G-protein-coupled membrane proteins. Messenger RNAs encoding both receptors have been detected in various tissues including the heart, lungs, kidneys and brain. The distribution of receptor subtypes is tissue specific [Martin et al. (1989) Biochem. Biophys. Res. Commun . 162 : 130-137]. The ET _A receptor appears to be selective for endothelin-1 and is predominant in cardiovascular tissue. The ET _B receptor predominates in non-cardiovascular tissues, including the central nervous system and kidneys, and interacts with three endothelin isopeptides [Sakurai et al. (1990) Nature 348 : 732-734. In addition, ET _A receptors occur in vascular smooth muscle, are associated with vasoconstriction and are associated with cardiovascular, renal and central nervous system diseases; ET _B receptors are located on the vascular endothelium and are associated with vasodilation [Takayanagi et al. (1991) FEBS Lttrs. 282 : 103-106].
[12] Due to the distribution of receptor types and the differential affinity of each isopeptide for each receptor type, the activity of the endothelin isopeptide varies in different tissues. For example, endothelin-1 inhibits ¹²⁵ I-labeled endothelin-1 binding in cardiovascular tissues 40-700 times more potently than endothelin-3. ¹²⁵ I-labeled endothelin-1 binding in non-cardiovascular tissues such as the kidney, adrenal gland and cerebellum is inhibited to the same extent by endothelin-1 and endothelin-3, which means that the ET _A receptor Predominant in cardiovascular tissue and ET _B receptor predominant in non-cardiovascular tissue.
[13] Endothelin plasma levels are elevated in certain disease states (see, eg, International PCT Application WO 94/27979 and US Pat. No. 5,382,569, which are incorporated herein by reference in their entirety). In healthy people, endothelin-1 plasma levels are about 0.26-5 pg / ml as measured by radioimmunoassay (RIA). Blood levels of endothelin and its precursors, big endothelin, are elevated in shock, myocardial infarction, vasoconstrictive angina, renal insufficiency and various connective tissue diseases. In patients undergoing hemodialysis or kidney transplants or suffering from cardiac shock, levels of myocardial infarction or pulmonary circulatory hypertension as high as 35 pg / ml have been observed [Stewart et al. (1991) Annals Internal Med. 114 : 464-469. Because endothelin appears to be a local regulatory factor rather than systemic, endothelin levels at the endothelial / smooth muscle interface are higher than circulating levels.
[14] Elevated levels of endothelin have also been observed in patients with ischemic heart disease . See Yasuda et al. (1990) Amer. Heart J. 119 : 801-806, Ray et al. (1992) Br. Heart J. 67 : 383-386. Circulating tissue endothelin immunoreactivity is more than doubled in patients with advanced atherosclerosis [Lerman et al. (1991) New Engl. J. Med. 325 : 997-1001]. Increased endothelin immunoreactivity is also seen in Burger disease [Kanno et al. (1990) J. Amer. Med. Assoc. 264 : 2868] and Raynaud's phenomenon [Ref . Zamora et al. (1990) Lancet 336 : 1144-1147. Increased circulating endothelin levels have been reported in patients with transdermal transluminal coronary angiogenesis (PTCA) [Tahara et al. (1991) Metab. Clin. Exp. 40 : 1235-1237; Sanjay et al. (1991) Circulation 84 (Suppl. 4) : 726], and those with pulmonary circulatory hypertension [Miyauchi et al. (1992) Jpn. J. Pharmacol. 58 : 279 P; Stewart et al. (1991) Ann. Internal Medicine 114 : 464-469. As such, there are clinical human data that support correlation with increased endothelin levels and multiple disease states.
[15] Endothelin agonists and antagonists
[16] Because endothelin is associated with certain disease states and with many physiological actions, there are compounds that can interfere with or enhance endothelin-related activity, such as endothelin-receptor interactions and vasoconstrictor activity. It is of interest. For example, the fermentation product of Streptomyces misakiensis, designated BE-18257B, has been identified as an ET _A receptor antagonist. BE-18257B inhibits ¹²⁵ I-labeled endothelin-1 binding in a concentration-dependent manner in cardiovascular tissues (IC ₅₀ is 1.4 μM in aortic smooth muscle, 0.8 μM in ventricular membrane and 0.5 μM in cultured aortic smooth muscle cells) One ET _B receptor is cyclic pentapeptide, cyclo (D-Glu-L-Ala-allo-Ile-L-Leu-D-Trp), which does not inhibit binding to receptors in tissues predominant at concentrations up to 100 μM. Cyclic pentapeptides associated with BE-18257B, such as cyclo (D-Asp-Pro-D-Val-Leu-D-Trp) (BQ-123), have been synthesized and shown to be active as ET _A receptor antagonists. [Reference: US Pat. No. 5,114,918, Ishikawa et al .; And EP A1 0 436 189, BANYU PHARMACEUTICAL CO., LTD (October 7, 1991). Studies measuring the inhibition of such endothelin-1 cyclic peptides by binding to endothelin-specific receptors show that the cyclic peptides preferentially bind to the ET _A receptor. Other peptides and non-peptidic ET _A antagonists have been identified [see, for example, 5,352,800, 5,334,598, 5,352,659, 5,248,807, 5,240,910, 5,198,548, 5,187,195. , 5,082,838]. These include other cyclic pentapeptides, acyl tripeptides, hexapeptide analogs, certain anthraquinone derivatives, indancarboxylic acids, certain N-pyriminylbenzenesulfonamides, certain benzenesulfonamides and certain naphthalenesulfonamides [Ref. Nakajima et al. (1991) J. Antibiot. 44 : 1348-1356; Miyata et al. (1992) J. Antibiot. 45 : 74-8; Ishikawa et al. (1992) J. Med. Chem. 35 : 2139-2142; U.S. Patent 5,114,918, Ishikawa et al .; EP A1 0 569 193; EP A1 0 558 258; EP A1 0 436 189, BANYU PHARMACEUTICAL CO., LTD (7 October 1991); Canadian Patent Application No. 2,067,288; Canadian Patent Application No. 2,071,193; US Patent No. 5,208,243; US Patent No. 5,270,313; U.S. Patent 5,612,359; US Patent No. 5,514,696; U.S. Patent 5,378,715, Cody et al. (1993) Med. Chem. Res. 3 : 154-162; Miyata et al. (1992) J. Antibiot. 45 : 1041-1046; Miyata et al. (1992) J. Antibiot. 45 : 1029-1040; Fujimoto et al. (1992) FEBS Lett. 305 : 41-44; Oshashi et al. (1992) J. Antibiot 45 : 1684-1685; EP A1 0 496 452; Clozel et al. (1993) Nature 365 : 759-761; International Patent Application WO 93/08799; Nishikibe et al. (1993) Life Sci. 52 : 717-724: and Benigni et al. (1993) Kidney Int. 44 : 440-444]. Many sulfonamides, which are endothelin peptide antagonists, are also described in US Pat. Nos. 5,464,853, 5,594,021, 5,591,761, 5,571,821, 5,514,691, 5,464,853, International PCT Application WO96 / 31492 and International PCT Application No. It is described in WO97 / 27979.
[17] In general, the compounds identified have activity at concentrations of about 50-100 μM or less as ET _A antagonists in in vitro assays. Many such compounds have also been shown to have activity in animal models in vivo.
[18] Endothelin antagonists and agonists as therapeutic agents
[19] In view of the many physiological actions of endothelin and its relationship with certain diseases, endothelin is considered to play an important role in this pathophysiological state. See, eg, Saito et al. (1990) Hypertension 15 : 734-738; Tomita et al. (1989) N. Engl. J. Med. 321 : 1127; Kurihara et al. (1989) J. Cardiovasc. Pharmacol. 13 (Suppl. 5) : S13-S17; Doherty (1992) J. Med. Chem. 35 : 1493-1508; Morel et al. (1989) Eur. J. Pharmacol. 167 : 427-428. More detailed knowledge of the function and structure of the endothelin peptide family should provide insight in the progression and treatment of this condition.
[20] Suitable formulations of these compounds in pharmaceutically acceptable vehicles are necessary for using the compounds in this manner.
[21] In standard in vitro assays evaluating endothelin antagonist or agonist activity, compounds exhibiting activity at IC ₅₀ or EC ₅₀ concentrations on the order of 10 ⁻⁴ or less have been recognized to have pharmacological utility [Reference: US Pat. No. 5,352,800] 5,334,598, 5,352,659, 5,248,807, 5,240,910, 5,198,548, 5,187,195, 5,082,838]. Because of this activity, the compound may cause hypertension, such as peripheral circulatory insufficiency, heart disease such as angina pectoris, cardiomyopathy, atherosclerosis, myocardial infarction, pulmonary circulatory hypertension, vasospasm, vascular restenosis, Raynaud's disease, brain Seizures, for example cerebral artery spasms, cerebral ischemia, late upper cerebral spasms after subarachnoid hemorrhage, asthma, bronchial contraction, renal insufficiency, especially post-ischemic renal insufficiency, cyclosporine renal toxicity, for example acute renal insufficiency Useful in the treatment of inflammatory diseases other than, and endotoxin shock caused by or in connection with endothelin, and diseases other than those involving endothelin. As noted above, many compounds, in particular sulfonamide compounds, are effective endothelin antagonists and are therefore ideal clinical subjects. In clinical use, there is a need for stable formulations and formulations suitable for various routes of administration.
[22] Current methods of preparing the sulfonamides have certain disadvantages. For example, certain steps in the synthetic route are known to result in dimerization of intermediates to reduce yields and purity. Second, because the compounds are hydrophobic, purification is difficult and typically requires impractical use of preparative HPLC or column chromatography. Finally, current methods are limited to the preparation of hydrophobic free sulfonamides, making sulfonamides difficult to formulate into aqueous based pharmaceutical compositions. Attempts to convert free sulfonamides to useful alkali metal salts using metal hydroxides or methoxides can lead to degradation of the compounds.
[23] It is therefore an object of the present invention to provide formulations of compounds having the ability to modulate the biological activity of one or more endothelin peptides. Yet another object is to provide formulations of compounds having use as specific endothelin antagonists. Another object is to provide formulations of compounds that specifically interfere with or inhibit the interaction of endothelin peptides with ET _A or ET _B receptors. Such formulations should be useful as therapeutic agents for the treatment of endothelin-mediated diseases and disorders. There is also a continuing need in the art for practical and effective methods for preparing salts of the desired sulfonamides.
[24] Salts of sulfonamide compounds
[25] Provided are sulfonamide derivatives for use in the formulations and methods provided herein, and methods of making sulfonamide derivatives. Sulfonamide derivatives are useful as endothelin receptor antagonists. Of interest are pharmaceutically acceptable derivatives including salts, esters, acids and bases, solvates, hydrates and prodrugs of sulfonamides. In particular, derivatives of neutral sulfonamide compounds are provided that provide formulations with unexpectedly higher stability than formulations containing the corresponding neutral compounds. Salts, in particular alkali metal salts, are preferred, more preferably sodium, including salts prepared from sodium compounds, including but not limited to sodium bicarbonate, where the resulting compound is a sodium salt and the resulting compound is a sodium hydrogen phosphate salt. Salts are more preferred. Most preferred are the sodium salts of each compound.
[26] Salt derivatives include alkali metal and alkaline earth metal salts including but not limited to sodium salts, potassium salts, lithium salts, calcium salts and magnesium salts; Transition metal salts such as zinc salts, copper salts, gold salts and silver salts, and other metal salts such as aluminum salts; Ammonium and substituted ammonium salts and organic amine salts, such as counter-ion salts of cations and polycationics, including but not limited to hydroxyalkyl and alkylamines; Salts of organic acids, including but not limited to acetate, lactate, malate, tartrate, citrate, ascorbate, succinate, butyrate, valerate and fumarate. Corresponding esters of acids are also contemplated herein.
[27] Preferred salts are: Acetate with trifluoroacetate, N, N'-dibenzylethylenediamine, chloroprocaine, choline, ammonia, diethanolamine and other hydroxyalkylamines, ethylenediamine, N-methyl Glucamine, procaine, N-benzylphenethylamine, 1-para-chlorobenzyl-2-pyrrolidin-1'-ylmethylbenzimidazole, diethylamine and other alkyl amines, piperazine, tris Oxymethyl) aminomethane, aluminum, calcium, lithium, magnesium, potassium, sodium hydrogen phosphate, disodium phosphate, sodium, zinc, barium, gold, silver and bismuth. Alkali metal salts, in particular sodium salts, are preferred herein.
[28] Derivatives, especially salts, preferably sodium salts, prepared from sulfonamides have the formula (1).
[29]
[30] Such sulfonamides are U.S. Pat. International Publication Nos. WO 96/31492 and WO 97/27979.
[31] In certain embodiments, the sulfonamide salt is provided wherein the salt is 4-chloro-3-methyl-5- (2- (6-methylbenzo [d] [1,3] dioxol-5-yl) acetyl)- 3-thienylsulfonamido) isoxazole, sodium salt; N ^2- (3-cyanomethyl-2,4,6-trimethylphenyl) -3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophencarboxamide, sodium salt ; N ^2- (3-acetyloxymethyl-2,4,6-trimethylphenyl) -3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophencarboxamide, sodium salt ; Or N ^2- (3-hydroxymethyl-2,4,6-trimethylphenyl) -3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophencarboxamide, sodium Choose not to salt.
[32] In particular, sulfonamides of formula (1) are alkyl substituted or unsubstituted by Ar ¹ or 5- or 6-membered substituted or unsubstituted aromatic or heteroaromatic rings, in particular 3- or 5-isoxazolyl and pyridazinyl, and It is also for example thiazolyl, for example 2-thiazolyl, pyrimidinyl, for example 2-pyrimidinyl, or a substituted benzene group, for example aryloxy substituted benzene group, or And cyclic or tricyclic carbocyclic or heterocyclic rings. Ar ¹ is selected from the following groups in certain embodiments:
[33]
[34] Wherein R is H, NH ₂ , halide, pseudohalide, alkyl, alkylcarbonyl, formyl, aromatic or heteroaromatic group, alkoxyalkyl, alkylamino, alkylthio, arylcarbonyl, aryloxy, arylamino, aryl Thio, haloalkyl, haloaryl, carbonyl, wherein the aryl and alkyl moieties are unsubstituted or substituted with any of the groups described above and have from about 1 to about 10-12 carbon atoms, preferably 1 to about 5 or 6 carbon atoms. Straight or branched. R is preferably H, NH ₂ , halide, CH ₃ , CH ₃ O or another aromatic group.
[35] Isoxazole is not 4-halo-isoxazole, 4-higher alkyl (C ₈ to C ₁₅ ) -isoxazole, or 4-biphenyl unsubstituted at 2 or 6 position on sulfonamide-bonded phenyl group this one, Ar ¹ is a N- (5- isoxazolyl) or N- (3- isoxazolyl) days if Ar ² is phenyl or naphthyl and the carbonyl is, Ar ² is the resulting sulfonamide, except that rather than not A group that allows binding of the endothelin peptide to the endothelin receptor at a concentration of less than about 100 μM by 50% compared to binding in the absence of sulfonamide.
[36] In particular, Ar ² includes naphthyl, phenyl, biphenyl, quinolyl, styryl, thienyl, furyl, isoquinolyl, pyrrolyl, benzofuryl, pyridinyl, thianaphthyl, indolyl, alkyl and alkenyl But not limited thereto, is a group unsubstituted or substituted with a substituent selected from one of the groups subject to the above clues. Naturally, the moieties indicated as substituents, including sulfonamide groups, may vary. Thus, for example, the compounds herein include groups comprising thiophen-3-sulfonamide and thiophene-2-sulfonamide.
[37] Sulfonamides are substituted or unsubstituted monocyclic or polycyclic aromatic or heteroaromatic sulfonamides such as benzene sulfonamide, naphthalene sulfonamide and thiophene sulfonamide. Particularly preferred sulfonamides are N-isoxazolyl sulfonamides. More particularly preferred sulfonamides of the above sulfonamides are those in which Ar ² is a heterocycle containing one ring, a plurality of rings or a fused ring, typically two or three rings and one or two heteroatoms in the ring or rings Things.
[38] In preferred sulfonamide derivatives, preferred sodium salts, Ar ² is thienyl, furyl, pyrrolyl, or benzofuryl, thianaph, which is a derivative or analog of the thienyl, furyl or pyrrolyl group or 4-biphenyl group as described below And a group such as nyl or indolyl, and Ar ¹ is preferably N- (5-isoxazolyl) or N- (3-isoxazolyl). Of particular interest here are the salts, in particular the sodium salts, for example the sodium salts of compounds in which Ar ² is a phenylacetyl-substituted thienyl, furyl, pyrrolyl group. Among these salts, particularly sodium salts, those in which Ar ² is thienyl, furyl or pyrrolyl, in particular those in which Ar ² is substituted with phenylacetyl and Ar ¹ isoxazolyl are preferred.
[39] In certain embodiments, preferred salts are 4-chloro-3-methyl-5- (2- (6-methylbenzo [d] [1,3] dioxol-5-yl) acetyl) -3-thienylsulfonami Isoxazoles, sodium salts; N ^2- (3-cyanomethyl-2,4,6-trimethylphenyl) -3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophencarboxamide, sodium salt ; N ^2- (3-acetyloxymethyl-2,4,6-trimethylphenyl) -3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophencarboxamide, sodium salt ; And N ^2- (3-hydroxymethyl-2,4,6-trimethylphenyl) -3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophencarboxamide, sodium Salt.
[40] One of the more preferred sulfonamides is N- (4-chloro-3-methyl-5-isoxazolyl) -2- [2-methyl-4,5- (methylenedioxy) phenylacetyl] thiophene-3-sulfone Sodium salt of an amide, and also herein 4-chloro-3-methyl-5- (2- (2- (6-methylbenzo [d]-[1,3] dioxol-5-yl) acetyl) -3 Thienylsulfonamido) isoxazole, also called sodium salt.
[41] Sulfonamide
[42] Also provided are sulfonamide compounds, and derivatives and formulations thereof as described herein. Sulfonamide compounds are active as endothelin receptor antagonists and provide enhanced resistance compared to sulfonamides known in the art. Preference is given to compounds of the formula (1) or pharmaceutically acceptable derivatives thereof, such as alkali metal salts, in particular sodium salts, in sulfonamides.
[43] Formula 1
[44]
[45] In the above formula,
[46] Ar ¹ is isoxazolyl;
[47] Ar ² is an expression ego;
[48] R ³ and R ⁴ are independently selected from the group consisting of hydrogen, halo, cyano, cyanoalkyl, C (O) R ⁴¹ , alkyl, alkenyl, cycloalkyl and aryl or together form an alkylene;
[49] W is O, NH or CH ₂ ;
[50] R ⁵ , R ⁶ and R ⁷ are each independently,
[51] (i) R ⁶ is hydrogen, unsubstituted alkyl, hydroxy, unsubstituted alkoxy, C (O) R ⁴¹ , carbamoyloxy or alkoxycarbonyloxy, and R ⁵ and R ⁷ are each independently hydrogen, unsubstituted Ring alkyl, hydroxy, C (O) R ⁴¹ , carbamoyloxy or alkoxycarbonyloxy;
[52] (ii) when at least one of R ³ and R ⁴ is not hydrogen, two form alkylenedioxy and the other is as defined in (i);
[53] Provided that at least one of R ⁵ , R ⁶ and R ⁷ is hydrogen;
[54] R ⁴⁵ is alkyl, C (O) R ⁴¹ , (CH ₂ ) _x OH and CH (OH) (CH ₂ ) _x CH ₃ (where x is 0 to 6), S (O) _n R ⁴¹ , wherein , n is 0 to 2) and C (= NR ⁴³ ) R ⁴¹ ;
[55] R ⁴¹ is alkyl, aryl, heteroaryl, aralkyl, heteroaralkyl, cycloalkyl, alkylamino, dialkylamino, arylamino, diarylamino, alkylsulfonylamino, arylsulfonylamino, alkylsulfonylalkylamino, Alkylsulfonylarylamino, arylsulfonylalkylamino or arylsulfonylarylamino;
[56] R ⁴³ is selected from hydroxy, alkoxy, alkyl and aryl,
[57] R ⁴¹ and R ⁴³ are unsubstituted or substituted with one or more substituents selected from Y being alkoxy, halide, pseudohalide, carboxyl, alkoxycarbonyl, aryloxycarbonyl or hydroxy, provided that N- (4-chloro-3 -Methyl-5-isoxazolyl) -2- (2,4,6-trimethylphenylaminocarbonyl) thiophen-3-sulfonamide, N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,4,6-trimethyl) phenylacetyl-3-thiophenylsulfonamide, N- (3,4-dimethyl-5-isoxazolyl) -2-[(2,4,6-trimethylphenoxy Carbonyl] thiophene-3-sulfonamide, N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(2,4,6-trimethylphenoxy) carbonyl] thiophene 3-sulfonamide or N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(2,4,6-trimethylphenoxy) carbonyl] thiophene-3-sulfonamide Excluded.
[58] In another embodiment, the sulfonamide is a compound wherein the compound is N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-methoxy-2,4,6-trimethylphenylaminocarbonyl) thiophene -3-sulfonamide, N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-hydroxy-2,4,6-trimethylphenylaminocarbonyl) thiophene-3-sulfone Amide, N- (4-chloro-5-methyl-3-isoxazolyl) -2- (3-hydroxy-2,4,6-trimethylphenylaminocarbonyl) thiophene-3-sulfonamide or N- (3,4-dimethyl-5-isoxazolyl) -2- (3-hydroxy-2,4,6-trimethylphenylaminocarbonyl) thiophene-3-sulfonamide is selected under additional conditions do.
[59] Thus, the sulfonamides provided herein are 2-acyl-3-thiophenesulfonamides. Corresponding 3-acyl-2-thiophenesulfonamides are also contemplated herein. Also provided are formulations and salts of the compounds described above as described herein.
[60] Formulations of Sulfonamide and Sulfonamide Salts
[61] Formulations of sulfonamide compounds having activity as endothelin antagonists for administration to mammals, including humans, are provided. In particular, formulations for parenteral, eg, intramuscular, intravenous and subcutaneous administration, oral administration, transdermal administration and other suitable routes of administration are provided. The formulations provide a means for consistently delivering an effective amount of the compound.
[62] Of interest are formulations of pharmaceutically acceptable derivatives of sulfonamides such as salts, esters, acids and bases, solvates, hydrates and prodrugs. In particular, derivatives of neutral sulfonamide compounds are obtained which result in a formulation having a greater solubility than a formulation containing the corresponding neutral compound. Salts, in particular alkali metal salts, more preferably sodium salts including salts prepared from sodium compounds, including but not limited to sodium bicarbonate, where the resulting product is a sodium salt and disodium hydrogen phosphate, the resulting compound is a sodium hydrogen phosphate salt This is preferred.
[63] The formulation is a composition suitable for administration by a preferred route and is a solvent, suspension, emulsion, tablet, dispersible tablet, pill, capsule, powder, anhydrous powder for inhalation, sustained release formulation, aerosol for nasal and respiratory delivery, transdermal delivery And patches for other suitable routes. The compositions should be suitable for oral administration, parenteral administration including subcutaneous, intramuscular or intravenous, transdermal administration and other selected routes as injectable aqueous or oily solvents or emulsions.
[64] Also provided are formulations containing lyophilized powders of sulfonamide derivatives, methods for their preparation, and reconstituted forms of lyophilized powders. Also provided are vials and ampoules containing powder and syringes and other suitable containers.
[65] In addition, one of the most preferred formulations for use in the methods provided herein is at a concentration substantially lower than the concentration that interacts with the compound that is ET _A selective, ie, the ET _B receptor (at least about 10 times, preferably 100 times lower). IC ₅₀ ) containing compounds that interact with the ET _A receptor. In particular, ET _A than less than about 10μM, preferably less than 1μM, more preferably one interaction with IC ₅₀ of less than 0.1μM, ET _B than compound to interact with more than about 10μM IC ₅₀ or ET _B than less than about 10μM , preferably less than 1μM, and more preferably, one interactive IC ₅₀ of less than 0.1μM, is ET _a preferred than a compound that interacts with at least about 10μM IC _50.
[66] Preferred formulations also include compounds that are ET _B receptor selective or that bind an ET _B receptor with an IC ₅₀ of less than about 1 μM. The ET _B selective compound interacts with the ET _B receptor at an IC ₅₀ concentration about 10 times lower than the concentration that interacts with the ET _A receptor.
[67] The formulations provided herein are for administration by the selected route and contain an effective concentration of pharmaceutically acceptable salts of the compounds described above. Hypertension, seizures, cardiovascular disease, heart diseases such as myocardial infarction, pulmonary circulatory hypertension, erythropoietin-mediated hypertension, respiratory diseases and inflammatory diseases such as asthma, bronchial contractions, ophthalmic diseases, for example Retinal perfusion of glaucoma and insufficiency, gastrointestinal disease, renal insufficiency, endotoxin shock, menstrual disease, obstetric disease, wounds, anaphylactic shock, hemorrhagic shock, and endothelin mediated physiological responses or associated vasoconstriction The present invention also provides a formulation that delivers an amount effective for the treatment of other diseases that can be treated or alleviated by administration of an endothelin antagonist or agonist.
[68] In one embodiment, the formulation is a capsule and tablet containing the sodium salt of sulfonamides described herein. Preferred formulations are those which deliver an amount effective for the treatment of hypertension or renal insufficiency. Effective amounts and concentrations are effective for alleviating the symptoms of the disease.
[69] In a more preferred embodiment, the formulation is a solid dosage form or gel, preferably a capsule or tablet. In a preferred embodiment, the formulation comprises an effective amount of at least one sodium hydrogen phosphate or sodium, preferably sodium salt, of at least one sulfonamide of formula (1), typically about 10 to 100%, preferably about 50 to 95%, more preferably Preferably about 75 to 85%, most preferably about 80 to 85%; Diluent or binder such as lactose or microcrystalline cellulose in an amount of about 0-25%, preferably 8-15%; Disintegrants, for example modified starch or cellulose polymers, in particular crosslinked sodium carboxymethyl cellulose, for example croscarmellose sodium (crosscarmellose sodium NF is available from FMC Corporation of Philadelphia, Pennsylvania under the trade name AC-DI- Commercially available as SOL) or sodium starch glycolate from about 0 to 10%, preferably from about 3 to 7%; And lubricants such as magnesium stearate, talc and calcium stearate from 0 to 5%, preferably from 0.1 to 2%. Disintegrants, such as croscarmellose sodium or sodium starch glycolate, provide for rapid destruction of the cellulosic matrix for immediate release of the active agent following degradation of the coating polymer. In all embodiments, the precise amounts of active and adjuvant components can be determined experimentally and correlate with the route of administration and the disease to be treated. Solid forms for administration as tablets are also contemplated herein.
[70] Preferred formulations are prepared from sterile lyophilized powders containing the sodium salt of sulfonamide. Lyophilized powders and methods of making such powders are also provided herein.
[71] In one embodiment, the composition comprises at least one sodium hydrogen phosphate or sodium, preferably sodium salt, and one or more of the following components of a sulfonamide compound of formula 1: a buffer such as sodium phosphate, potassium phosphate, sodium citrate or citric acid potassium; Solubilizing agents such as LABRASOL (polyethylene glycol-8 caprylic acid capric acid glycerides sold by Gatefosce SA of France), dimethylsulfoxide (DMSO), bis (trimethylsilyl) acetamide, Ethanol, propylene glycol (PG) or polyvinylpyrrolidine (PVP); And freezing containing sugars or other carbohydrates such as sorbitol or dextrose (typically about 1% to 20%, preferably about 5% to 15%, more preferably about 5% to 10%) Provided in dried solid form.
[72] For administration, the lyophilized powder is mixed with a suitable carrier such as phosphate buffered saline (typically to obtain about 100 to 500 mg, preferably 250 mg of a single or multiple dose formulation).
[73] In a preferred embodiment other than where the formulation is designed for parenteral administration, the composition comprises at least one sodium hydrogen phosphate or sodium, preferably sodium salt of at least one sulfonamide compound of formula (1); Buffers such as sodium phosphate, potassium phosphate, sodium citrate or potassium citrate; And sugars such as sorbitol or dextrose. In a preferred embodiment described in detail herein, the formulation comprises at least one sodium salt of the sulfonamide compound of formula 1; Sodium phosphate buffer; And dextrose. Dextrose can be added in the form of a sterile dextrose solution readily available from suppliers known to those skilled in the art.
[74] How to use
[75] Methods of using the formulations to modulate the interaction of endothelin peptides with ET _A and / or ET _B receptors are provided. The method is carried out by contacting the receptor simultaneously with one or more formulated pharmaceutically acceptable salts of sulfonamides, preferably formulated sodium salts of sulfonamides, prior to or in contact with the endothelin peptide. do.
[76] Provided are methods for inhibiting the binding of endothelin peptides to endothelin receptors. These methods are performed by contacting the receptor simultaneously or subsequently with a formulation of one or more pharmaceutically acceptable salts of the compounds provided herein prior to contacting the receptor with the endothelin peptide.
[77] Treating or associated with endothelin-mediated diseases including but not limited to hypertension, asthma, shock, hypertension, glaucoma, retinal perfusion of dysfunction, and conditions mediated in certain ways by endothelin peptides Or a disease that is alleviated by the administration of an endothelin antagonist or agonist.
[78] In particular, methods are provided for treating endothelin-mediated diseases by administering an effective amount of a formulation of a pharmaceutically acceptable salt of sulfonamide, a prodrug of sulfonamide or other suitable derivative. In particular, by administering an effective amount of a formulation of one or more pharmaceutically acceptable salts of a compound provided herein in a pharmaceutically acceptable carrier, hypertension, cardiovascular disease, heart disease such as myocardial infarction, pulmonary circulatory pressure, erythropoietin Mediated hypertension, respiratory and inflammatory diseases, such as asthma, bronchial contractions, ophthalmic diseases, gastrointestinal diseases, kidney dysfunction, endotoxin shock, menstrual disease, obstetric disease, wounds, anaphylactic shock, hemorrhagic shock, And methods for treating diseases other than those associated with endothelin mediated physiological responses. Preferred methods of treatment are methods of treating hypertension and renal insufficiency.
[79] A more preferred method of treatment is that the formulation has less than about 10 μM, preferably less than about 5 μM, more preferably less than about 1 μM, even more preferably less than 0.1 μM, most preferred, the interaction of endothelin-1 with the ET _A receptor Preferably it is a method containing one or more compounds which inhibit at IC ₅₀ of less than 0.05 μM. Other preferred methods are those wherein the formulation contains pharmaceutically acceptable salts of one or more compounds that are ET _A selective or pharmaceutically acceptable salts of one or more compounds that are ET _B selective. The method in which the compound is ET _A selective is for treating diseases such as hypertension; The method in which the compound is ET _B selective is for the treatment of diseases such as asthma requiring bronchiectasis.
[80] In practicing the method, hypertension, cardiovascular disease, heart disease such as myocardial infarction, respiratory disease such as asthma, inflammatory disease, eye disease, gastrointestinal disease, renal insufficiency, immunosuppressant-mediated kidney Oral, intravenous, topical or localized to treat diseases other than those associated with vasoconstriction, erythropoietin-mediated vasoconstriction, endotoxin shock, anaphylactic shock, hemorrhagic shock, pulmonary circulatory boost and endothelin mediated physiological responses An effective amount of a formulation containing a pharmaceutically acceptable salt of a topically formulated compound in a therapeutically effective concentration is administered to a person exhibiting one or more symptoms of the disease. The amount is effective to alleviate or eliminate one or more symptoms of the disease.
[81] Also provided are methods for identifying and isolating endothelin receptor subtypes. In particular, methods are provided for detecting, identifying and isolating endothelin receptors using the compounds described. In particular, methods are provided for detecting, identifying and isolating endothelin receptors using the compounds provided herein.
[82] Also provided are methods for identifying compounds suitable for use in treating a particular disease based on preferential affinity for a particular endothelin receptor subtype.
[83] Reducing symptoms of, or offsetting the effects of, endothelin peptide, or the endothelin peptide, on the ET receptor of the endothelin peptide, wherein the packaging material, the formulation contained within the packaging material, comprises a compound having an IC ₅₀ of less than about 10 μM. A formulation provided herein effective to inhibit binding of a label, and a label indicating that the formulation is used to counteract the effects of endothelin, to treat an endothelin-mediated disease, or to inhibit the binding of an endothelin peptide to the ET receptor. To provide a product that contains.
[84] Manufacturing method
[85] Also provided are methods of preparing alkali metal salts of hydrophobic free sulfonamides. The method includes dissolving free sulfonamide in an organic solvent, washing the dissolved free sulfonamide with a saturated solution of alkali metal salts, and recovering the alkali metal salt of sulfonamides from the organic phase. Preferred organic solvents are ethyl acetate or THF. Preferred alkali metals are sodium, potassium, calcium or magnesium, with sodium being most preferred. According to a preferred embodiment, the process uses saturated sodium bicarbonate or sodium carbonate as the alkali metal salt solution. Sodium bicarbonate is most preferred.
[86] Recovery preferably comprises drying the salt solution in an organic solvent, concentrating the salt, crystallizing the salt in one or more organic, water-immiscible solvents and collecting the sulfonamide salt by filtration. Preferred organic, water-immiscible solvents are dichloromethane and ethers. The methods provided herein may further comprise purifying the sulfonamide salt after recovery.
[87] An embodiment of this method is 4-chloro-3-methyl-5- (2- (6-methylbenzo [d] [1,3] dioxol-5-yl) acetyl) -3-thienylsulfonamido) Isoxazole, sodium salt; N ^2- (3-cyanomethyl-2,4,6-trimethylphenyl) -3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophencarboxamide, sodium salt ; N ^2- (3-acetyloxymethyl-2,4,6-trimethylphenyl) -3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophencarboxamide, sodium salt ; And N ^2- (3-hydroxymethyl-2,4,6-trimethylphenyl) -3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophencarboxamide, sodium It is particularly useful for preparing salts.
[88] Alkali metal salts of sulfonamides are provided, in particular the salts provided by the process. Preferred sulfonamide salts are 4-chloro-3-methyl-5- (2- (2- (6-methylbenzo [d] [1,3] dioxol-5-yl) acetyl) -3-thienylsulfone Amido) isoxazole, sodium salt.
[89] Justice
[90] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. All patents and publications referenced herein are incorporated by reference.
[91] As used herein, endothelin (ET) peptides include peptides that substantially have the amino acid sequence of endothelin-1, endothelin-2, or endothelin-3 and act as effective endogenous vasoconstrictor peptides.
[92] As used herein, an endothelin-mediated disease is a disease caused by abnormal endothelin activity or a disease in which a compound that inhibits endothelin activity has a therapeutic use. Such diseases include, but are not limited to, hypertension, cardiovascular disease, asthma, inflammatory diseases, ophthalmic diseases, menstrual diseases, obstetric diseases, gastrointestinal diseases, kidney dysfunction, pulmonary circulatory pressure, endotoxin shock, anaphylactic shock or hemorrhagic shock Does not. Endothelin-mediated diseases also include diseases resulting from treatment with agents such as erythropoietin and immunosuppressive agents that increase endothelin levels.
[93] As used herein, an effective amount of a compound that treats a particular disease is an amount sufficient to relieve or reduce in certain ways the symptoms associated with the disease. Such amounts may be administered as a single dose or may be administered according to a useful regime. The amount can cure the disease, but is typically administered to alleviate the symptoms of the disease. Typically, repeated administrations are necessary to achieve the alleviation of the desired symptoms.
[94] As used herein, an endothelin agonist is a compound associated with or increasing or exhibiting the biological activity of an endothelin peptide.
[95] As used herein, endothelin antagonists are compounds such as drugs or antibodies that inhibit endothelin-stimulated vasoconstriction and contraction and other endothelin-mediated physiological responses. Antagonists can act by interfering with the interaction of endothelin with endothelin-specific receptors or by disrupting the physiological response or bioactivity of the endothelin isopeptide, eg vasoconstriction. Thus, as used herein, endothelin antagonists, as assessed by assays known in the art, interfere with endothelin-stimulated vasoconstriction or endothelin and endothelin-specific receptors such as ET Interfere with interaction with the _A receptor.
[96] Potency and potency of antagonists can be assessed using methods known in the art. For example, endothelin agonist activity can be confirmed by its ability to stimulate vasoconstriction of isolated aortic or portal portal vein fragments . Borges et al. (1989) "Tissue selectivity of endothelin" Eur, J. Pharmacol. 165 .: 223-230]. Endothelin antagonist activity can be assessed by its ability to interfere with endothelin-induced vasoconstriction. Exemplary assays are described in the Examples. As noted above, the preferred IC ₅₀ concentration ranges are described in connection with assays in which test compounds are incubated at 4 ° C. with ET receptor-bearing cells. Confirmation of the data presented for the assay where the incubation step was performed at the less desirable 24 ° C. Of course, for the purpose of comparison these concentrations are somewhat higher than the concentrations measured at 4 ° C.
[97] As used herein, the biological activity or bioactivity of an endothelin includes activities that are induced, enhanced or affected in vivo by the endothelin. It also includes the ability to bind specific receptors to induce functional responses such as vasoconstriction. This can be assessed by an in vivo assay or an in vitro assay as exemplified herein. Related activities include, but are not limited to vasoconstriction, vasorelaxation and bronchiectasis. For example, ET _B receptors appear to be expressed in endothelial cells of blood vessels and can mediate vasodilation and other such responses, whereas endothelin-1-specific ET _A receptors occur on smooth muscle and are associated with vasoconstriction do. To measure or detect such activity, assays known to those of skill in the art can be used to assess such activity. See, eg, Spokes et al. (1989) J. Cardiovasc. Pharmacol. 13 (Suppl. 5) : S191-S192; Spinella et al. (1991) Proc. Natl. Acad. Sci. USA 88 : 7443-7446; Cardell et al. (1991) Neurochem. Int. 18 : 571-574); And Examples herein].
[98] As used herein, bioavailability means absorption and absorption range. Methods of measuring bioavailability are well known to those skilled in the art. For example, the bioavailability of a compound described herein can be determined experimentally by administering the compound to an animal, then taking a blood sample over time and measuring the blood concentration of the compound. In vivo half-life (t _1/2 ) is defined as the time it takes for the blood's concentration of the compound to decrease by half. Estimates of the area under the curve for intravenous administration can be used to estimate the area under the curve for oral administration to obtain bioavailability data. See, e.g., Milo Gibal (1991) Biopharmaceutics and Pharmacology, 4th edition. Lea and Sediger].
[99] As used herein, efficacy refers to the maximum effect that can be produced by a compound. Efficacy can be measured by methods known in the art. For example, this can be measured by the properties of the compound and its receptor-agonist system and reflected in the flatness of the concentration-action curve. In vivo efficacy means efficacy measured in an animal model. For example, the in vivo efficacy of the compounds described herein can be measured by alleviation of hypoxia-induced pulmonary circulatory boost in rats. In this relationship, in vivo efficacy refers to the compound's ability to restore elevated pulmonary aortic pressure to normal [see, eg, DiCarlo et al. (1995) Am. J. Physiol. 269 : L690-L697].
[100] As used herein, IC ₅₀ refers to the amount, concentration, or dosage of a particular test compound in an assay that measures the response, achieving 50% inhibition of maximal response, such as binding of endothelin to tissue receptor. do.
[101] As used herein, EC ₅₀ refers to the dose, concentration, or amount of a particular test compound that directs a dose-dependent response to 50% of the maximum expression of a particular response that is induced, elicited, or enhanced by that particular test compound. it means.
[102] As used herein, sulfonamide that is ET _A selective means sulfonamide that exhibits an IC ₅₀ that is about 10 times lower with respect to the ET _A receptor than the ET _B receptor.
[103] As used herein, sulfonamide that is ET _B selective means sulfonamide that exhibits an IC ₅₀ that is about 10 times lower with respect to the ET _B receptor than the ET _A receptor.
[104] As used herein, pharmaceutically acceptable salts, esters, hydrates, solvates or other derivatives of the compounds can be prepared by one skilled in the art using known methods for derivatization and have substantial toxic effects. Salts, esters and other derivatives that produce compounds that can be administered to an animal or human without a pharmaceutically active or prodrug. Pharmaceutically acceptable salts include salts of alkali metals and alkaline earth metals, including but not limited to sodium salts, potassium salts, lithium salts, calcium salts and magnesium salts; Transition metal salts such as zinc salts, copper salts and aluminum salts; Ammonium and substituted ammonium salts and organic amine salts such as, for example, ionic salts of polycationics, including but not limited to hydroxyalkyl amines and alkylamines; Salts of organic acids, including but not limited to acetate, lactate, malate, tartrate, citrate, ascorbate, succinate, butyrate, valerate and fumarate. Corresponding esters are also contemplated herein.
[105] Preferred pharmaceutically acceptable salts are N, N'-dibenzylethylenediamine, chloroprocaine, choline, ammonia, diethanolamine and other hydroxyalkylamines, ethylenediamine, N-methylglucamine, procaine, N -Benzylphenethylamine, 1-para-chlorobenzyl-2-pyrrolidin-1'-ylmethylbenzimidazole, diethylamine and other alkylamines, piperazine, tris (hydroxymethyl) aminomethane, aluminum , Salts of calcium, lithium, magnesium, potassium, sodium hydrogen phosphate, disodium phosphate, sodium, zinc, barium, gold, silver and bismuth. Sodium salts, in particular the sodium salt of each compound, are most preferred herein.
[106] As used herein, “sodium salts” means salts of sodium compounds in which the counterion may comprise Na ⁺ and may comprise other ions, such as HPO ₄ ^2- ; "Sodium salt" (rather than sodium salts) specifically means salts in which Na ⁺ is a counterion.
[107] As used herein, treatment means a method in which the symptoms of an abnormality, disorder or disease of the body are alleviated or otherwise beneficially changed. Treatment also includes the pharmaceutical use of the compositions herein, eg, as a contraceptive preparation.
[108] As used herein, alleviation of the symptoms of a particular disorder by the administration of a particular pharmaceutical composition means alleviation, which may be due to or associated with the administration of the composition, whether permanent or temporary, persistent or temporary.
[109] As used herein, substantially pure means standard analytical methods used by those skilled in the art to assess purity, such as thin layer chromatography (TLC), gel electrophoresis and high performance liquid chromatography (HPLC). Sufficiently homogeneous to determine if impurities are not detectable or readily detectable when measured by, or sufficient so that further purification does not detectably change the physical and chemical properties of the material, eg, enzymes and biological activity. Means pure. Methods of purifying compounds for preparing substantially chemically pure compounds are known to those skilled in the art. However, substantially chemically pure compounds may be mixtures of stereoisomers. In such cases, further purification may increase the specific activity of the compound.
[110] As used herein, biological activity refers to the physiological consequences of the in vivo activity of a compound or the in vivo administration of a compound, composition or mixture other than. Thus, biological activity includes therapeutic and pharmaceutical activity of compounds, compositions and mixtures.
[111] As used herein, increased stability of a formulation refers to the active ingredient present in the formulation at a given time period after preparation of the formulation by assays known to those skilled in the art, such as high performance liquid chromatography, gas chromatography, and the like. It means that the percentage is significantly higher than the percentage of active ingredient present in other formulations in the same period after preparation of the formulation. In this case, the former formulation is said to have increased stability compared to the latter formulation.
[112] As used herein, prodrugs are metabolized upon in vivo administration or converted to the biological, pharmaceutical or therapeutically active form of the compound. To prepare prodrugs, pharmaceutically active compounds are modified so that the active compounds can be regenerated by metabolic processes. Prodrugs can be designed to alter the stability or transport properties of the drug, to block side effects or toxicity, to improve the flavor of the drug, or to change other properties or characteristics of the drug. With the knowledge of pharmacodynamic processes and drug metabolism in vivo, the skilled artisan can devise prodrugs of compounds, as long as the pharmaceutically active compounds are known [see, for example, Nogrady (1985). Medicinal Chemistry A Biochemical Approach , Oxford University Press, New York, pages 388-392. For example, succinyl-sulfatazole is a prodrug of 4-amino-N- (2-thiazoyl) benzenesulfonamide (sulfatazole) that exhibits altered transport properties.
[113] As used herein, acid isostere refers to a group that is significantly ionized at physiological pH. Examples of suitable acid isosteres include sulfo, phosphono, alkylsulfonylcarbamoyl, tetrazolyl, arylsulfonylcarbamoyl or heteroarylsulfonylcarbamoyl.
[114] As used herein, halo or halide is a halogen atom; F, Cl, Br and I.
[115] As used herein, pseudohalides are compounds that behave substantially similarly to halides. Such compounds can be used in the same manner as halides (where X is halogen, eg Cl or Br) and treated in the same manner. Pseudohalides include, but are not limited to, cyanide, cyanate, thiocyanate, selenocyanate and azide.
[116] As used herein, haloalkyl refers to lower alkyl radicals in which one or more hydrogen atoms are replaced by halogen, including but not limited to chloromethyl, trifluoromethyl, 1-chloro-2-fluoroethyl, and the like. do.
[117] As used herein, alkyl refers to an aliphatic hydrocarbon group that is straight or branched chain, preferably having about 1 to 12 carbon atoms in the chain. Preferred alkyl groups mean lower alkyl groups which are alkyl containing 1 to about 6 carbon atoms in the chain. By side chain is meant that one or more lower alkyl groups, such as methyl, ethyl or propyl, are attached to the linear alkyl chain. Alkyl groups may be independently substituted or unsubstituted by one or more groups, including but not limited to halo, carboxy, formyl, sulfo, sulfino, carbamoyl, amino and imino. Exemplary alkyl groups include methyl, ethyl and propyl.
[118] As used herein, the term lower describes alkyl, alkenyl and alkynyl groups containing about 6 or fewer carbon atoms. It is also used to describe aryl groups or heteroaryl groups containing up to 6 atoms in the ring. Lower alkyl, lower alkenyl and lower alkynyl refer to carbon chains having less than about 6 carbons. Preferred embodiments of the compounds provided herein comprising alkyl, alkenyl or alkynyl moieties include lower alkyl, lower alkenyl and lower alkynyl moieties.
[119] As used herein, alkenyl refers to an aliphatic hydrocarbon group that contains a carbon-carbon double bond and may be straight or branched chain with about 2 to about 10 carbon atoms in the chain. Preferred alkenyl groups have from 2 to about 4 carbon atoms in the chain. Side chain means that one or more lower alkyl or lower alkenyl groups are bonded to a linear alkenyl chain. Alkenyl groups may be independently or unsubstituted by one or more groups, including but not limited to halo, carboxy, formyl, sulfo, sulfino, carbamoyl, amino and imino. Exemplary alkenyl groups include ethenyl, propenyl and butenyl.
[120] As used herein, alkynyl refers to an aliphatic hydrocarbon group that contains a carbon-carbon triple bond and may be straight or branched chain with about 2 to about 10 carbon atoms in the chain. By side chain is meant that one or more lower alkyl, alkenyl or alkynyl groups are bonded to the linear alkynyl chain. Exemplary alkynyl groups are ethynyl.
[121] As used herein, aryl means a monocyclic or multicyclic hydrocarbon ring system containing 3 to 15 or 16, preferably 5 to 10 carbon atoms. Aryl groups include, but are not limited to, phenyl, substituted phenyl, naphthyl, substituted naphthyl, where the substituent is lower alkyl, halogen or lower alkoxy. Preferred aryl groups are lower aryl groups containing less than 7 carbons in the ring structure.
[122] As used herein, the nomenclature alkyl, alkoxy, carbonyl, and the like are used as are generally familiar to those skilled in the art. Eg, as used herein, it refers to a saturated carbon chain containing one or more carbons; The chain may be straight or branched, contain cyclic moieties or be cyclic.
[123] As used herein, cycloalkyl means saturated cyclic carbon chains; Cycloalkenyl and cycloalkynyl refer to cyclic carbon chains each containing one or more unsaturated double or triple bonds. The cyclic moiety of the carbon chain may comprise one ring or two or more fused rings.
[124] As used herein, cycloalkenyl means a non-aromatic monocyclic or multicyclic ring system containing carbon-carbon double bonds and having about 3 to about 10 carbon atoms. Exemplary monocyclic cycloalkenyl rings include cyclopentenyl or cyclohexenyl; Cyclohexenyl is preferred. An exemplary multicyclic cycloalkenyl ring is norbornylenyl. Cycloalkenyl groups may be independently substituted by one or more halo or alkyl.
[125] As used herein, "haloalkyl" refers to a lower level wherein one or more hydrogen atoms may be substituted by halogen, including but not limited to chloromethyl, trifluoromethyl, 1-chloro-2-fluoroethyl, and the like. It means an alkyl radical.
[126] As used herein, "haloalkoxy" means RO-, wherein R is a haloalkyl group.
[127] As used herein, “carboxamide” means a group of formula R _p CONH _{2 wherein} R is selected from alkyl or aryl, preferably lower alkyl or lower aryl, and p is 0 or 1.
[128] As used herein, "alkylaminocarbonyl" means -C (O) NHR, wherein R is hydrogen, alkyl, preferably lower alkyl or aryl, preferably lower alkyl.
[129] As used herein, "dialkylaminocarbonyl" as used herein refers to -C (O) NR'R wherein R 'and R are independently selected from alkyl or aryl, preferably lower alkyl or lower aryl. and; "Carboxamide" means a group of the formula NR'COR.
[130] As used herein, "alkoxycarbonyl" as used herein means -C (O) OR where R is alkyl, preferably lower alkyl or aryl, preferably lower alkyl.
[131] As used herein, "alkoxy" and "thioalkyl" refer to RO- and RS-, wherein R is alkyl, preferably lower alkyl or aryl, preferably lower aryl.
[132] As used herein, "haloalkoxy" means RO-, wherein R is a haloalkyl group.
[133] As used herein, "aminocarbonyl" means -C (O) NH ₂ .
[134] As used herein, cycloalkyl means saturated cyclic carbon chains; Cycloalkenyl and cycloalkynyl refer to cyclic carbon chains comprising one or more unsaturated triple bonds. The cyclic moiety of the carbon chain may comprise one ring or two or more fused rings.
[135] As used herein, alkylenedioxy means an -O-alkyl-O- group in which the alkyl group is as described above. Substituted analogs of alkylenedioxy means alkylenedioxy in which one or two oxygen atoms are substituted by atoms or groups of atoms that behave similarly, such as S, N, NH, Se. Exemplary substituted alkylenedioxy groups are ethylenebis (sulfandiyl). Alkylenethioxyoxy is -S-alkyl-O-, -O-alkyl-S and alkylenedioxy is -S-alkyl-S-.
[136] As used herein, heteroaryl means an aromatic monocyclic or fused ring in which one or more carbon atoms in the ring are replaced by an element other than carbon, such as nitrogen, oxygen, or sulfur. Preferred cyclic groups contain one or two fused rings and comprise about 3 to about 7 members in each ring. Similar to an "aryl group", a heteroaryl group may or may not be substituted by one or more substituents. Exemplary heteroaryl groups include pyrazinyl, pyrazolyl, tetrazolyl, furyl, (2- or 3-) thienyl, (2-, 3- or 4-) pyridyl, imidazoyl, pyrimidinyl, isoxa Zolyl, thiazolyl, isothiazolyl, quinolinyl, indolyl, isoquinolinyl, oxazolyl and 1,2,5-oxadiazolyl. Preferred heteroaryl groups include 5-6 membered nitrogen-containing rings such as pyrimidinyl.
[137] As used herein, alkoxycarbonyl refers to an alkyl-O-CO- group. Exemplary alkoxycarbonyl groups include methoxy- and ethoxycarbonyl.
[138] As used herein, carbamoyl means -CONH ₂ . As with all groups described herein, these groups may or may not be substituted. Substituted carbamoyls are those wherein Y ² and Y ³ are independently hydrogen, alkyl, cyano (lower alkyl), aralkyl, heteroaralkyl, carboxy (lower alkyl), carboxy (aryl substituted lower alkyl), carboxy (carboxy substituted Lower alkyl), carboxy (hydroxy substituted lower alkyl), carboxy (heteroaryl substituted lower alkyl), carbamoyl (lower alkyl), alkoxycarbonyl (lower alkyl) or alkoxycarbonyl (aryl substituted lower alkyl) -CONY ² Y ³ in the stage, includes the same group, Y ² and Y ³ may be only one of the hydrogen and one of Y ² and Y ^3-carboxy (lower alkyl), carboxy (aryl substituted lower alkyl), When carbamoyl (lower alkyl), alkoxycarbonyl (lower alkyl) or alkoxycarbonyl (aryl substituted lower alkyl), the other of Y ² and Y ³ is hydrogen or alkyl. Hydrogen, alkyl, cyano (lower alkyl), aralkyl, heteroaralkyl, carboxy (lower alkyl), carboxy (aryl substituted lower alkyl) and carbamoyl (lower alkyl) are preferred independently for Y ² and Y ³ Do.
[139] As used herein, its corresponding N- (4-halo-3-methyl-5-isoxazolyl), N- (4-halo-5-methyl-3-isoxazolyl), N- (3 , 4-dimethyl-5-isoxazolyl), N- (4-halo-5-methyl-5-isoxazolyl), N- (4-halo-3-methyl-5-isoxazolyl), N- (4,5-dimethyl-3-isoxazolyl) derivatives are the same as those for which Ar ² is specifically described, but Ar ¹ is N- (4-halo-5-methyl-3-isoxazolyl), N- ( 4-halo-5-methyl-3-isoxazolyl), N- (3,4-dimethyl-5-isoxazolyl), N- (4-halo-5-methyl-3-isoxazolyl), N A compound which is-(4-halo-3-methyl-5-isoxazolyl) or N- (4,5-dimethyl-3-isoxazolyl), where halo is a halide, preferably Cl or Br it means.
[140] As used herein, abbreviations for protecting groups, amino acids, and other compounds are in accordance with the IUPAC-IUB Committee on their customary conventions, recognized abbreviations, or biochemical nomenclature, unless otherwise specified. 1972) Biochem. 11 : 942-944].
[141] A. Salts of Sulfonamide Compounds
[142] In the embodiments described in detail herein, the salts of the sulfonamide compounds, preferably the sodium salts, for use in the compositions and methods provided herein are salts wherein Ar ¹ is isoxazole and the compound is represented by Formula 2a or 2b.
[143]
[144]
[145] In the above formula,
[146] R ¹ and R ²
[147] (Iii) each independently H, NH ₂ , NO ₂ , halide, pseudohalide, alkyl, alkenyl, alkynyl, aryl, aralkyl, heteroaryl, alkoxy, alkylamino, alkylthio, alkyloxy, haloalkyl, alkyl Sulfinyl, alkylsulfonyl, aryloxy, arylamino, arylthio, arylsulfinyl, arylsulfonyl, haloalkyl, haloaryl, alkoxycarbonyl, alkylcarbonyl, aminocarbonyl, arylcarbonyl, formyl, substituted Or unsubstituted amido and substituted or unsubstituted ureido, wherein the alkyl, alkenyl and alkynyl moieties comprise from 1 to about 14 carbon atoms and are straight or branched or cyclic and the aryl moiety is From about 4 to about 16 carbon atoms, provided that R ² is not a halide or pseudohalide;
[148] (Ii) together form-(CH ₂ ) _n , wherein n is 3 to 6;
[149] (Iii) together to form 1,3-butadienyl, provided that the isoxazole is not 4-halo-isoxazole, 4-higher alkyl (C ₈ to C ₁₅ ) -isoxazole or the compound is a sulfonamide-bond Ar ² is phenyl or when Ar ¹ is N- (5-isoxazolyl) or N- (3-isoxazole), unless unsubstituted 4-biphenylsulfonamide at the 2 or 6 position on the phenyl group; It's not naphthyl.
[150] In a preferred embodiment herein, R ¹ and R ² are each independently selected from alkyl, lower alkenyl, lower alkynyl, lower haloalkyl, halide, pseudohalide or H, except that R ² is not a halide do.
[151] In certain embodiments, the sulfonamide salt is provided wherein the salt is 4-chloro-3-methyl-5- (2- (6-methylbenzo [d] [1,3] dioxol-5-yl) acetyl)- 3-thienylsulfonamido) isoxazole, sodium salt; N ^2- (3-cyanomethyl-2,4,6-trimethylphenyl) -3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophencarboxamide, sodium salt ; N ^2- (3-acetyloxymethyl-2,4,6-trimethylphenyl) -3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophencarboxamide, sodium salt ; Or N ^2- (3-hydroxymethyl-2,4,6-trimethylphenyl) -3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophencarboxamide, sodium Choose not to salt.
[152] In certain embodiments described in detail herein, Ar ² is 4-biphenyl or a single ring heterocycle, in particular a 5-membered ring, or contains at least one, in particular one heteroatom, selected from S, O and NR ^{42 in the} ring Fused bicyclic or tricyclic heterocycle, wherein R ⁴² contains up to about 30, preferably 1 to 10, more preferably 1 to 6 carbon atoms, hydrogen, alkyl, al Kenyl, alkynyl, aryl, alkylaryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl, cycloalkynyl, C (O) R ¹⁵ and S (O) _n R ¹⁵ where n is 0 to 2); R ¹⁵ is hydrogen, alkyl, alkenyl, alkynyl, aryl, alkylaryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl, cycloalkynyl; R ⁴² and R ¹⁵ are hydrogen, halide, pseudohalide, alkyl, alkoxy, alkenyl, alkynyl, aryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl, cycloalkynyl, OH, CN, C (O) R ¹⁶ , CO ₂ R ¹⁶ , SH, S (O) _n R ¹⁶ , where n is 0 to 2, NHOH, NR ¹² R ¹⁶ , NO ₂ , N ₃ , OR ¹⁶ , R ¹² Substituted or unsubstituted with one or more substituents each independently selected from Z which is NCOR ¹⁶ and CONR ¹² R ¹⁶ ; R ¹⁶ is hydrogen, alkyl, alkenyl, alkynyl, aryl, alkylaryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl or cycloalkynyl; R ¹² independently selected from R ⁴² and Z is hydrogen, alkyl, alkenyl, alkynyl, aryl, alkylaryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl, cycloalkynyl, C (O ) R ¹⁷ and S (O) _n R ¹⁷ , wherein n is 0 to 2; R ¹⁷ is selected from hydrogen, alkyl, alkenyl, alkynyl, aryl, alkylaryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl or cycloalkynyl; R ⁴² , R ¹² , R ¹⁵ and R ¹⁶ may be further substituted with the groups described for Z.
[153] In a preferred embodiment herein, R ⁴² is aryl, for example phenyl or alkyl phenyl, hydrogen or lower alkyl.
[154] Thus, in the compounds provided herein, Ar ² is thienyl, furyl and pyrrolyl, benzofuryl, benzopyrrolyl, benzothienyl, benzo [b] furyl, benzo [b] thienyl, and indolyl (benzo [b] Pyrrolyl) and 4-biphenyl, and Ar ¹ is preferably N- (5-isoxazolyl) or N- (3-isoxazolyl). Sulfonamide is N-isoxazolyl sulfonamide and has the formula 3a or 3b; The compound is 4-biphenylsulfonamide wherein Ar ¹ is preferably N- (5-isoxazolyl) or N- (3-isoxazolyl):
[155]
[156]
[157] In the above formula,
[158] X is S, O or NR ¹¹ wherein R ¹¹ contains about 30 or less, preferably 1 to 10, more preferably 1 to 6 carbon atoms, hydrogen, alkyl, alkenyl, alkynyl, Aryl, alkylaryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl, cycloalkynyl, C (O) R ¹⁵ and S (O) _n R ¹⁵ , where n is 0 to 2 Is selected from; R ¹⁵ is hydrogen, alkyl, alkenyl, alkynyl, aryl, alkylaryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl, cycloalkynyl; R ¹¹ and R ¹⁵ are hydrogen, halide, pseudohalide, alkyl, alkoxy, alkenyl, alkynyl, aryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl, cycloalkynyl, OH, CN, C (O) R ¹⁶ , CO ₂ R ¹⁶ , SH, S (O) _n R ¹⁶ , where n is 0 to 2, NHOH, NR ¹² R ¹⁶ , NO ₂ , N ₃ , OR ¹⁶ , R ¹² Substituted or unsubstituted with one or more substituents each independently selected from Z which is NCOR ¹⁶ and CONR ¹² R ¹⁶ ; R ¹⁶ is hydrogen, alkyl, alkenyl, alkynyl, aryl, alkylaryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl or cycloalkynyl; R ¹² is independently selected from R ¹¹ and Z, hydrogen, alkyl, alkenyl, alkynyl, aryl, alkylaryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl, cycloalkynyl, C (O) R ¹⁷ and S (O) _n R ¹⁷ , wherein n is 0 to 2; R ¹⁷ is hydrogen, alkyl, alkenyl, alkynyl, aryl, alkylaryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl or cycloalkynyl; R ¹¹ , R ¹² , R ¹⁵ and R ¹⁶ may each be further substituted with the groups described for Z, and R ¹¹ is preferably hydrogen, aryl, eg phenyl or alkyl phenyl, or lower alkyl] to be.
[159] In one of the embodiments described in detail herein, Ar ² is as described below for the thienyl, furyl or pyrrolyl group, including thienyl, furyl, pyrrolyl, or benzo [b] derivatives such as benzo [b] thienyl And a group that is the same derivative or analog, and Ar ¹ is N- (5-isoxazolyl) or N- (3-isoxazolyl). Ar ² is a group of formula 4a or 4b:
[160]
[161]
[162] In the above formula,
[163] X is O, S or NR ¹¹ , wherein R ¹¹ is as defined above; this is an analog of the group of formula 4 which may be substituted at a specific or all positions or forms a fused aromatic, aliphatic or heterocyclic ring Or a derivative);
[164] R ⁸ , R ⁹ and R ¹⁰
[165] (i) each containing hydrogen or up to about 50, typically up to 30, more typically up to 20 carbon atoms, each independently hydrogen, halide, pseudohalide, alkyl, alkoxy, alkenyl, alkynyl, Aryl, aryloxy, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl, cycloalkynyl, OH, CN, C (O) R ¹⁸ , (OAc) CH = CHR ^18- , CO ₂ R ¹⁸ , SH, (CH ₂ ) _r C (O) (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r (CH = CH) _s (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r C (O) (CH = CH) _s (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r (CH = CH) _s C (O) (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r NH (CH = CH) _s (CH ₂ ) _n R ¹⁸ , C = N (OH) (CH ₂ ) _r R ¹⁸ , (CH ₂ ) _r (CH = CH) _s NH (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r C (O) NH ( CH ₂ ) _n R ¹⁸ , C (O) (CH ₂ ) _r NH (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r NH (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r R ¹⁸ , S (O ) _m R ¹⁸ , HNOH, NR ¹⁸ R ¹⁹ , NO ₂ , N ₃ , OR ¹⁸ , R ¹⁹ NCOR ¹⁸ and CONR ¹⁹ R ¹⁸ [where m is 0 to 2 and s, n and r are each independently 0 To 6, preferably Crab is 0-3, R ¹⁹ is hydrogen, alkyl, alkenyl, alkynyl, aryl, alkylaryl, alkoxy, aryloxy, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl, cycloalkynyl , C (O) R ²⁰ and S (O) _n R ²⁰ , where n is 0 to 2; R ¹⁸ and R ²⁰ are independently hydrogen, alkyl, alkenyl, alkynyl, aryl, heteroaryl, alkylaryl, heterocyclyl, alkoxy, aryloxy, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl and cycloalky Selected from nil; The groups described above for R ⁸ , R ⁹ and R ¹⁰ are hydrogen, halide, pseudohalide, alkyl, alkoxy, alkenyl, alkynyl, aryl, aryloxy, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cyclo Alkenyl, cycloalkynyl, OH, CN, C (O) R ²¹ , CO ₂ R ²¹ , SH, S (O) _n R ²¹ , where n is 0 to 2, NHOH, NR ²² R ²¹ , Or substituted or unsubstituted with Z selected from the group consisting of NO ₂ , N ₃ , OR ²¹ , R ²² NCOR ²¹ and CONR ²² R ²¹ ; R ²² is hydrogen, alkyl, alkenyl, alkynyl, aryl, alkylaryl, heterocyclyl, aralkyl, alkoxy, aralkoxy, cycloalkyl, cycloalkenyl, cycloalkynyl, C (O) R ²³ and S ( O) _n R ²³ wherein n is 0 to 2; R ²¹ and R ²³ are independently selected from hydrogen, alkyl, alkenyl, alkynyl, aryl, alkylaryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl and cycloalkynyl. Provided that R ⁸ is NR ¹⁸ R ¹⁹ , OR ¹⁸ , R ¹⁹ NCOR ¹⁸ and CONR ¹⁹ R ¹⁸ CO ₂ R ¹⁸ , (CH ₂ ) _r NH (CH = CH) _s (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r (CH = CH) _s NH (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r C (O) NH (CH ₂ ) _n R ¹⁸ , C (O) (CH ₂ ) _r NH (CH ₂ ) _{When n} R ¹⁸ , (CH ₂ ) _r NH (CH ₂ ) _n R ¹⁸ or (CH ₂ ) _r R ¹⁸ and R ¹⁸ is an aryl group containing 5 or 6 members, the aryl group is two or more substituents, preferably Preferably have one substituent at the 2 position associated with binding to thienyl, furyl or pyrrolyl;
[166] (ii) two of R ⁸ , R ⁹ and R ¹⁰ together with the carbon to which they are respectively bonded form an aryl ring, aromatic ring, heteroaromatic ring, carbocyclic ring or heterocyclic ring, which is saturated or unsaturated Each of which is substituted with one or more substituents independently selected from Z and comprises about 3 to about 16 members, preferably 3 to about 10 members, more preferably 5 to 7 members; The other of R ⁸ , R ⁹ and R ¹⁰ is as defined for (i); Heteroatoms are NR ¹¹ , O or S, provided that Ar ² is 5-halo-3-lower alkylbenzo [b] thienyl, 5-halo-3-lower alkylbenzo [b] furyl, 5-halo-3 It is not a lower alkylbenzo [b] pyrrolyl.
[167] In the embodiments provided herein, the alkyl, alkynyl and alkenyl moieties of each described substituent are straight or branched, bicyclic or cyclic, preferably having from about 1 to about 10 carbons; In more preferred embodiments they have 1 to 6 carbons. Aryl, bicyclic, aromatic rings and heterocyclic groups may have 3 to 16 members, generally 3 to 7 members, more often 5 to 7 members, in the ring and may be single or fused rings. Ring size and carbon chain length bind the resulting molecules and retain activity as an endothelin antagonist or agonist such that the resulting compound binds the endothelin peptide to the endothelin receptor in the absence of sulfonamide at a concentration of less than about 100 μM. The amount is selected to inhibit binding by 50% compared to
[168] In a preferred embodiment of interest herein, R ⁹ and R ¹⁰ are hydrogen, halide or methyl, more preferably hydrogen or halide and R ⁸ is CO ₂ R ¹⁸ , (CH ₂ ) _r C (O) (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r (CH = CH) _s (CH ₂ ) _n R ¹⁸ , C = N (OH) (CH ₂ ) _r R ¹⁸ , (CH ₂ ) _r C (O) (CH = CH) _s (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r (CH = CH) _s C (O) (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r NH (CH = CH) _s (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r (CH = CH) _s NH (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r C (O) NH (CH ₂ ) _n R ¹⁸ , C (O) (CH ₂ ) _r NH (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r NH (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r R ¹⁸ , wherein R ⁸ is CO ₂ R ¹⁸ , (CH ₂ ) _r C (O) NH (CH ₂ ) _n R ¹⁸ , C (O) (CH ₂ ) _n NH (CH ₂ ) _r R ¹⁸ , (CH ₂ ) _r NH (CH ₂ ) _n R ¹⁸ or (CH ₂ ) _r When R ¹⁸ and R ¹⁸ are phenyl, the phenyl group is substituted at two or more positions, preferably at least one of these positions is ortho.
[169] In a preferred compound, R ¹⁸ is aryl or heteroaryl having 5 or 6 members in the ring, more preferably phenyl or pyrimidinyl, most preferably phenyl.
[170] In the most preferred compounds herein, R ¹⁸ is substituted at one or more positions, most preferably at least one substituent is phenyl at the ortho position, and R ⁹ and R ¹⁰ are each hydrogen, halide or lower alkyl, preferably Is hydrogen and R ⁸ is C (O) NHR ¹⁸ , C (O) CH ₂ R ¹⁸ , (CH ₂ ) R ¹⁸ , provided that when R ⁸ is C (O) NHR ¹⁸ , there are two phenyl groups It should have at least a substituent, preferably one of the substituents is in the ortho position.
[171] In other preferred embodiments, Ar ² is benzo [b] thienyl, benzo [b] furyl or indolyl (benzo [b] pyrrolyl), provided the benzene ring is substituted and the substituent is 5-halo, 3-lower Not alkyl. Preferred substituents on the benzene ring are alkylenedioxy, in particular methylenedioxy, preferably 3,4-methylenedioxy, ethylenedioxy, aryl, in particular phenyl, dimethylamino, diethylamino, benzyl, alkoxy, especially lower alkoxy, Examples include, but are not limited to, one or more selected from methoxy and ethoxy, halides, and alkyl, preferably lower alkyl.
[172] In a preferred compound herein, R ² is preferably selected from alkyl, lower alkenyl, lower alkynyl, lower haloalkyl or H; R ¹ is halide or lower alkyl, more preferably R ¹ is bromine or chlorine, methyl or ethyl. As demonstrated by in vitro binding assays, in the most active compounds provided herein, R ¹ is bromine or chlorine. For in vivo use, R ¹ is preferably chlorine.
[173] In the most preferred embodiment herein, the formulation contains a sodium salt of the compound wherein R ⁸ is phenylacenyl. Among the compounds described herein, it is desirable to inhibit or increase the endothelin-mediated activity to about 50% at concentrations of less than about 10 μM. Inhibiting or increasing the endothelin-mediated activity to about 50% at a concentration of less than about 1 μM, more preferably less than about 0.1 μM, even more preferably less than about 0.01 μM, most preferably less than about 0.001 μM. More preferred. Note that the IC ₅₀ concentration as measured in the in vitro assay as described below is a nonlinear function of the culture temperature. Preferred values recited herein refer to assays performed at 4 ° C. When the assay is performed at 24 ° C., a rather high (see Table 1) IC ₅₀ concentration is observed. Thus, the preferred IC ₅₀ concentration is about 10 times higher.
[174] In addition, one of the most preferred compounds for use in the methods provided herein is ET _A selective, i.e. at substantially lower concentrations (more than about 10 times, preferably about 100 times lower than interacting with the ET _B receptor) in concentration) to interact with ET _a receptors. In particular, ET _A than less than about 10μM, preferably less than 1μM, more preferably one interaction with IC ₅₀ of less than 0.1μM, ET _B than compound to interact with more than about 10μM IC ₅₀ or ET _B than less than about 10μM , preferably less than 1μM, and more preferably, one interactive IC ₅₀ of less than 0.1μM, is ET _a preferred than a compound that interacts with at least about 10μM IC _50.
[175] Preferred compounds also include compounds that are ET _B receptor selective or that bind an ET _B receptor with an IC ₅₀ of less than about 1 μM. The ET _B selective compound interacts with the ET _B receptor at an IC ₅₀ concentration about 10 times lower than the concentration that interacts with the ET _A receptor. In the compound, R ² is selected from alkyl, lower alkenyl, lower alkynyl, lower haloalkyl, halide or H; R ¹ is halide or lower alkyl, and in preferred embodiments R ¹ is bromine or chlorine, preferably chlorine; R ⁹ and R ¹⁰ are independently selected from hydrogen, lower alkyl, preferably methyl or ethyl, or halide, and R ⁸ (see, eg, Formulas 3 and 4), which is a substituent at position 5, is aryl or hetero Aryl or heterocycle, in particular phenyl and isoxazole, substituted or unsubstituted with Z, which is preferably lower alkyl or halide.
[176] 1. Ar ² is thiophene, pyrrole, furan, benzo [b] thiophene, indolyl (benzo [b] pyrrole) or benzo [b] furan.
[177] One of the sulfonamide salts provided herein is a salt of a compound of formula 5a, 5b, 5c or 5d.
[178]
[179]
[180]
[181]
[182] In the above formula,
[183] R ¹ and R ²
[184] (Iii) each independently H, NH ₂ , NO ₂ , halide, pseudohalide, alkyl, alkenyl, alkynyl, aryl, aralkyl, heteroaryl, alkoxy, alkylamino, alkylthio, haloalkoxy, haloalkyl, alkyl Sulfinyl, alkylsulfonyl, aryloxy, arylamino, arylthio, arylsulfinyl, arylsulfonyl, aminocarbonyl, arylaminocarbonyl, haloalkyl, haloaryl, alkoxycarbonyl, alkylcarbonyl, arylcarbonyl , Formyl, substituted or unsubstituted amido and substituted or unsubstituted ureido, wherein the alkyl, alkenyl and alkynyl moieties are straight or branched chains containing from 1 to about 10 carbon atoms, and the aryl moiety Contains about 4 to about 14 carbon atoms, provided that R ² is not halide, pseudohalide or higher alkyl;
[185] (Ii) together form-(CH ₂ ) _n , wherein n is 3 to 6;
[186] (Iii) together form 1,3-butadienyl;
[187] X is S, O or NR ¹¹ [wherein R ¹¹ contains up to about 30, preferably 1 to 10, more preferably 1 to 6 carbon atoms and is hydrogen, alkyl, alkenyl, alkynyl , Aryl, alkylaryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl, cycloalkynyl, C (O) R ¹⁵ and S (O) _n R ¹⁵ , where n is 0 to 2 ) Is selected from; R ¹⁵ is hydrogen, alkyl, alkenyl, alkynyl, aryl, alkylaryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl, cycloalkynyl; R ¹¹ and R ¹⁵ are hydrogen, halide, pseudohalide, alkyl, alkoxy, alkenyl, alkynyl, aryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl, cycloalkynyl, OH, CN, C (O) R ¹⁶ , CO ₂ R ¹⁶ , SH, S (O) _n R ¹⁶ , where n is 0 to 2, NHOH, NR ¹² R ¹⁶ , NO ₂ , N ₃ , OR ¹⁶ , R ¹² Unsubstituted or substituted with one or more substituents each independently selected from Z consisting of NCOR ¹⁶ and CONR ¹² R ¹⁶ ; R ¹⁶ is hydrogen, alkyl, alkenyl, alkynyl, aryl, alkylaryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl or cycloalkynyl; R ¹² is independently selected from R ¹¹ and Z, hydrogen, alkyl, alkenyl, alkynyl, aryl, alkylaryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl, cycloalkynyl, C (O) R ¹⁷ and S (O) _n R ¹⁷ , wherein n is 0 to 2; R ¹⁷ is hydrogen, alkyl, alkenyl, alkynyl, aryl, alkylaryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl or cycloalkynyl; R ¹¹ , R ¹² , R ¹⁵ and R ¹⁶ may each be further substituted with a group described for Z, and R ¹¹ is preferably hydrogen, aryl, eg phenyl or alkyl phenyl or lower alkyl] ;
[188] R ⁸ , R ⁹ and R ¹⁰ , each containing up to about 50 hydrogen atoms, generally up to about 30 carbon atoms, and more generally up to 20 carbon atoms, are each independently as described above, more preferably,
[189] (i) hydrogen, halide, pseudohalide, alkyl, alkoxy, alkenyl, alkynyl, aryl, aryloxy, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl, cycloalkynyl, OH, CN, C (O) R ¹⁸ , (OAC) CH = CHR ¹⁸ , CO ₂ R ¹⁸ , SH, (CH ₂ ) _r C (O) (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r (CH = CH) _s (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r C (O) (CH = CH) _s (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r (CH = CH) _s C (O) (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r NH (CH = CH) _s (CH ₂ ) _n R ¹⁸ , C = N (OH) (CH ₂ ) _r R ¹⁸ , (CH ₂ ) _r (CH = CH) _s NH (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r C (O) NH (CH ₂ ) _n R ¹⁸ , C (O) (CH ₂ ) _r NH (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r NH (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r R ¹⁸ , S (O) _m R ¹⁸ , HNOH, NR ¹⁸ R ¹⁹ , NO ₂ , N ₃ , OR ¹⁸ , R ¹⁹ NCOR ¹⁸ and CONR ¹⁹ R ¹⁸ [ Wherein m is 0-2, s, n and r are each independently 0-6, preferably 0-3, R ¹⁹ is hydrogen, alkyl, alkenyl, alkynyl, aryl, alkylaryl, alkoxy, Aryloxy, heterocyclyl, aralkyl, aralkoxy, tetra Isoalkyl, cycloalkenyl, cycloalkynyl, C (O) R ²⁰ and S (O) _n R ²⁰ , where n is 0 to 2; R ¹⁸ and R ²⁰ are independently hydrogen, alkyl, alkenyl, alkynyl, aryl, heteroaryl, alkylaryl, heterocyclyl, alkoxy, aryloxy, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl or cycloalky Selected from nil;
[190] R ⁸ is C (O) R ¹⁸ , (OAC) CH = CHR ¹⁸ , CO ₂ R ¹⁸ , (CH ₂ ) _r C (O) (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r (CH = CH) _s (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r C (O) (CH = CH) _s (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r (CH = CH) _s C (O) (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r NH (CH = CH) _s (CH ₂ ) _n R ¹⁸ , C = N (OH) (CH ₂ ) _r R ¹⁸ , (CH ₂ ) _r (CH = CH) _s NH (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r C (O) NH (CH ₂ ) _n R ¹⁸ , C (O) (CH ₂ ) _r NH (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r Selected from NH (CH ₂ ) _n R ¹⁸ and (CH ₂ ) _r R ¹⁸ where m is 0 to 2, s, n and r are each independently 0 to 6, preferably 0 to 3, R ¹⁸ is aryl, preferably phenyl, provided that R ⁸ is (CH ₂ ) _r C (O) NH (CH ₂ ) _n R ¹⁸ , C (O) (CH ₂ ) _r NH (CH ₂ ) _{n ^{r 18, (CH 2) r}} NH (CH 2) n r 18, (CH 2) r r , if ¹⁸ days, particularly if r is 0 and / or n is 0 and r ¹⁸ is aryl, especially biphenylyl, r ¹⁸ Must have at least two substituents, preferably at least one ortho substituent;
[191] The groups described above for R ⁸ , R ⁹ and R ¹⁰ are hydrogen, halide, pseudohalide, alkyl, alkoxy, alkenyl, alkynyl, aryl, aryloxy, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cyclo Alkenyl, cycloalkynyl, OH, CN, C (O) R ²¹ , CO ₂ R ²¹ , SH, S (O) _n R ²¹ , where n is 0 to 2, NHOH, NR ²² R ²¹ , Or substituted or unsubstituted with Z selected from the group consisting of NO ₂ , N ₃ , OR ²¹ , R ²² NCOR ²¹ and CONR ²² R ²¹ ; R ²² is hydrogen, alkyl, alkenyl, alkynyl, aryl, alkylaryl, heterocyclyl, aralkyl, alkoxy, aralkoxy, cycloalkyl, cycloalkenyl, cycloalkynyl, C (O) R ²³ and S ( O) _n R ²³ wherein n is 0 to 2; R ²¹ and R ²³ are independently selected from hydrogen, alkyl, akenyl, alkynyl, aryl, alkylaryl, heterocyclyl, aralkyl, aralkoxy, cycloalkyl, cycloalkenyl or cycloalkynyl] ;
[192] (ii) two of R ⁸ , R ⁹ and R ¹⁰ are each substituted with one or more substituents independently selected from Z and are from about 3 to about 16 members, preferably 3 to about 10 members, more preferably 5 to 7 To form a saturated or unsaturated aryl ring, aromatic ring, heteroaromatic ring, carbocyclic ring or heterocyclic ring containing a member; The other of R ⁸ , R ⁹ and R ¹⁰ is selected from the group described for R ⁹ and R ¹⁰ in (i); Heteroatoms are NR ¹¹ , O or S, provided that Ar ² is 5-halo-3-lower alkylbenzo [b] thienyl, 5-halo-3-lower alkylbenzo [b] furyl, 5-halo-3 -Not lower alkyl benzo [b] pyrrolyl.
[193] In this embodiment, as such, Ar ² is represented by Formulas 4a and 4b, which are analogs of compounds of Formula 4 which may be substituted at specific or all positions and the substituents form fused aromatic, aliphatic or heterocyclic rings:
[194] Formula 4a
[195]
[196] Formula 4b
[197]
[198] In the above formula,
[199] X is NR ¹¹ , wherein R ¹¹ is hydrogen or up to about 30, preferably contains 1 to 10, more preferably 1 to 6 carbon atoms and is selected as described above, O or S;
[200] R ⁸ , R ⁹ and R ¹⁰ are selected as above.
[201] In the embodiments provided herein, when R ⁸ , R ⁹ and R ¹⁰ are selected from (i) above, R ⁸ is preferably (CH ₂ ) _r C (O) (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r NH (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r NH (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r (CH = CH) _s (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r C (O) (CH = CH) _s (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r (CH = CH) _s C (O) (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r (CH = CH ) _s NH (CH ₂ ) _n R ¹⁸ , C = N (OH) (CH ₂ ) _r R ¹⁸ , (CH ₂ ) _r C (O) NH (CH ₂ ) _n R ¹⁸ , C (O) (CH ₂ ) _r NH (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r NH (CH = CH) _s (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r C (O) NH (CH ₂ ) _n R ¹⁸ , ( CH ₂ ) _r NH (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r R ¹⁸ , wherein R ⁸ is (CH ₂ ) _r C (O) NH (CH ₂ ) _n R ¹⁸ , (CH ₂ ) _r C (O) NH (CH ₂ ) _n R ¹⁸ or (CH ₂ ) _r R ¹⁸ and R ¹⁸ is phenyl, the phenyl group is substituted at two or more positions, preferably at least one of these positions Is ortho.
[202] In this preferred compound, R ¹⁸ is aryl or heteroaryl, preferably having 5 or 6 members in the ring, more preferably phenyl or pyrimidinyl, most preferably phenyl. R ⁹ and R ¹⁰ are preferably hydrogen, halide, lower alkyl or halo lower alkyl.
[203] More preferred compounds provided herein are compounds wherein the alkyl, alkynyl and alkenyl moieties are straight or branched, acylyl or cyclic and have from about 1 to about 10 carbons; In certain more preferred embodiments, they have 1 to 6 carbons and they may have less than 6 carbons. Aryl, homocyclic and heterocyclic groups may have 3 to 16 members, generally 3 to 7 members, more often 5 to 7 members, in the ring and may be single or fused rings. Ring size and carbon chain length are chosen such that the resulting molecules exhibit activity as endothelin antagonists or agonists, as demonstrated by in vitro or in vivo tests, in particular the tests exemplified herein.
[204] In this preferred embodiment, R ¹ and R ² are independently preferably selected from alkyl, lower alkenyl, lower alkynyl, lower haloalkyl, halide, pseudohalide and H, provided that R ² is halide or pseudohalide And not preferred alkyl in the preferred embodiment.
[205] In a preferred embodiment, X is S, O or NR ¹¹ wherein R ¹¹ is aryl, hydrogen or lower alkyl, preferably substituted or unsubstituted aryl, in particular phenyl, preferably lower alkyl or halogen hydrogen or lower alkyl Is phenyl unsubstituted or substituted with; R ¹ is hydrogen, halide, pseudohalide, lower alkyl or lower haloalkyl, most preferably halide; R ² is hydrogen, lower alkyl or lower haloalkyl.
[206] Aryl groups are substituted or unsubstituted with groups such as alkyl, alkoxy, alkoxyalkyl, halogen, alkylenedioxy, in particular methylenedioxy, amino, nitro and other such groups. The alkyl substituent is preferably lower alkyl, more preferably alkyl having 1 to 3 carbon atoms.
[207] In a more preferred embodiment two of R ⁹ and R ¹⁰ are hydrogen, halide or lower alkyl and R ⁸ is C (O) NHR ¹⁸ or C (O) CH ₂ R ¹⁸ , wherein R ¹⁸ is at least two positions More preferably are phenyl groups having at least one substituent in the ortho position and also 3,4 or 4,5 alkylenedioxy substituents. In this more preferred embodiment X is S.
[208] In all embodiments, R ¹ is preferably halide, H, CH ₃ or C ₂ H ₅ and R ² is H, CH ₃ , C ₂ H ₅ , C ₂ F ₅ or CF ₃ . In even more preferred embodiments, R ¹ is preferably Br, Cl or CH ₃ ; R ² is H, CH ₃ , C ₂ H ₅ or CF ₃ .
[209] In other embodiments, two of R ⁸ , R ⁹ and R ¹⁰ are Ar ² benzo [b] thienyl, benzo [b] furyl or indolyl, provided one or more substituents are present and they are 5-halo and Form a non-lower alkyl ring and the other of R ⁸ , R ⁹ and R ¹⁰ is aryl, (CH ₂ ) _r R ¹⁸ , C (O) R ¹⁸ , CO ₂ R ¹⁸ , NR ¹⁸ R ¹⁹ , SH , S (O) _n R ¹⁸ , wherein n is 0 to 2, NHOH, NO ₂ , N ₃ , OR ¹⁸ , R ¹⁹ NCOR ¹⁸ and CONR ¹⁹ R ¹⁸ . Ar ² may also be substituted with the groups described for R ⁸ , R ⁹ and R ¹⁰ , preferably alkyl, alkoxy, alkoxyalkyl, aryl, alkylaryl, aminoalkyl, arylamino, aryl-substituted amino and NR ¹¹ is selected from.
[210] In embodiments requiring an ET _B antagonist, R ⁸ and R ¹⁰ are H or lower alkyl and R ⁹ in the ring is preferably a 3-14 membered, more preferably 5-7 membered heterocyclic or aromatic ring It is preferable to include. In particular, when X is S, R ⁸ and R ¹⁰ are H or lower alkyl and R ⁹ comprises phenyl substituted with an aryl group, in particular with, for example, a lower alkyl substituent. The aryl moiety is substituted with alkyl, alkoxy, alkoxyalkyl, halogen, alkylenedioxy, in particular methylenedioxy, amino, nitro and the same groups as the above groups. The alkyl substituent is preferably lower alkyl, more preferably alkyl having 1 to 3 carbon atoms.
[211] When X is NR ¹¹ , R ¹¹ is aryl, in particular unsubstituted phenyl or substituted phenyl, for example isopropylphenyl.
[212] Another preferred compound that is ET _B active is a compound of Formula 4b wherein Ar ² is R ⁹ is aryl or Z-substituted aryl, especially phenyl and Z is lower alkyl or lower alkoxy.
[213] In all embodiments of all compounds herein, R ¹ is preferably halide or lower alkyl, most preferably Br and the compound is 2- or 3-sulfonamide, in particular thiophene sulfonamide, in connection with formula (4) to be. In certain embodiments provided herein, Ar ² is a benzo [b] thienyl, benzo [b] furyl or indolyl (benzo [b] pyrrolyl) group and the compound provided herein is preferably benzo [b] thienyl -Benzo [b] furyl- or indolylsulfonamide. Benzo [b] thienyl, benzo [b] furyl or indolyl 2- or 3-sulfonamide are one of the preferred compounds herein. The benzo [b] thienyl, benzo [b] furyl or indolyl 2- or 3-sulfonamides provided herein are provided provided that the benzo group has one or more substituents and the substituents are not 5-halo and 3-lower alkyl groups. To select.
[214] Of particular interest are compounds in which Ar ² is a phenyl-, benzothienyl, benzofuryl or indolyl [benzopyrrolyl] group or substituted phenylaminocarbonylthienyl, substituted phenylamino in which Ar ² is present with two or more substituents A carbonylfuryl, substituted aminocarbonylpyrrolyl group or Ar ² is phenylacetylthienyl, phenylacetylfuryl or phenylacetylpyrrolyl or acetoxystyrylthienyl, acetoxystyrylfuryl or acetoxystyrylpyrrolyl group Salts of 3, in particular sodium salts.
[215] Most preferred compounds herein are those having an IC ₅₀ of less than 0.1 μM, more preferably less than 0.01 μM, most preferably for the ET _A receptor in the assays exemplified herein, as measured in the Examples. Salts of compounds having less than 0.001 μM (see, eg, Table 1 for representative experimental results). When measured at 24 ° C., the IC ₅₀ concentration is rather high (2-10 fold; see Table 1 for some comparisons).
[216] One of the preferred compounds of interest herein is the salt of a compound wherein Ar ² is formula (6).
[217]
[218] In the above formula,
[219] M is (CH ₂ ) _m C (O) (CH ₂ ) _r , (CH ₂ ) _m C (O) NH (CH ₂ ) _r , (CH ₂ ) _m (CH = CH) (CH ₂ ) _r , ( CH ₂ ) _m C (O) (CH ₂ ) _s NH (CH ₂ ) _r , (CH ₂ ) _m (CH = CH) (CH ₂ ) _r , C = N (OH) (CH ₂ ) _r , (CH ₂ ) _m C (O) (CH = CH) _s NH (CH ₂ ) _r , CH (OH) (CH ₂ ) _r , CH (CH ₃ ) C (O) (CH ₂ ) _r , CH (CH ₃ ) C (O) (CH ₂ ) _m (CH = CH) (CH ₂ ) _r , (CH ₂ ) _r , (CH ₂ ) _r O, C (O) O (where m, s and r are each independently 0 to 6, preferably 0 to 3), and more preferably M is (CH ₂ ) _m C (O) (CH ₂ ) _r , (CH ₂ ) _m C (O) NH (CH ₂ ) _r , (CH ₂ ) _m (CH = CH) (CH ₂ ) _r , (CH ₂ ) _m C (O) (CH ₂ ) _s NH (CH ₂ ) _r , (CH ₂ ) _m (CH = CH) (CH ₂ ) _r , C = N (OH) (CH ₂ ) _r , CH (OH) (CH ₂ ) _r , (CH ₂ ) _r , (CH ₂ ) _r O, C (O) O;
[220] R ³¹ , R ³² , R ³³ , R ³⁴ and R ³⁵
[221] (Iii) each independently H, OH, NHR ³⁸ , CONR ³⁸ R ³⁹ , wherein R ³⁸ and R ³⁹ are each independently hydrogen, alkyl, alkenyl, alkynyl, aryl, haloalkyl, alkylaryl, heterocyclyl , Aralkyl, aralkoxy, alkoxy, aryloxy, cycloalkyl, cycloalkenyl and cycloalkynyl, preferably hydrogen, lower alkyl, lower alkoxy and lower haloalkyl), NO ₂ , cyano, Halides, pseudohalides, alkyl, alkenyl, alkynyl, aryl, aralkyl, heteroaryl, alkoxy, alkylamino, alkylthio, haloalkyl, alkylsulfinyl, alkylsulfonyl, alkoxycarbonyl, alkylcarbonyl, alkenyl Thio, alkenylamino, alkenyloxy, alkenylsulfinyl, alkenylsulfonyl, alkoxycarbonyl, arylaminocarbonyl, alkylaminocarbonyl, aminocarbonyl, (alkylaminocarbonyl) alkyl, carboxyl, carboxyalkyl, Carboxyalkenyl, alkylsulfonylaminoalkyl, cya Alkyl, acetyl, acetoxy alkyl, hydroxyalkyl, alkyloxy-alkoxy, hydroxyalkyl, (acetoxy) alkoxy, (hydroxy) alkoxy and formyl or selected from;
[222] (Ii) two or more of R ³¹ , R ³² , R ³³ , R ³⁴ and R ³⁵ , which substitute adjacent carbons on the ring, together with one or more hydrogens are substituted or unsubstituted with halides, lower alkyl, lower alkoxy or halo lower alkyl Substituted alkylenedioxy, alkylenedioxyoxy or alkylenedithioxy (ie, -O- (CH ₂ ) _n -O-, -S- (CH ₂ ) _n -O-, -S- (CH ₂ ) _n -S-, wherein n is 1 to 4, preferably 1 or 2, and the remaining of R ³¹ , R ³² , R ³³ , R ³⁴ and R ³⁵ are as defined in (i);
[223] Provided that when M is (CH ₂ ) _m C (O) NH (CH ₂ ) _r , at least two of R ³¹ , R ³² , R ³³ , R ³⁴ and R ³⁵ are not hydrogen.
[224] M is most preferably Is selected from.
[225] In general, however, in all of the above compounds wherein Ar ² is of formula 5 or 6 or R ⁸ is an aryl group regardless of the choice of M, it is preferred that the aryl substituent has at least one substituent or at least one substituent at the ortho position. desirable. When the substituents combine to form an alkylenedioxy (or analog thereof) in which one or two oxygens are substituted by S, the aryl is preferably at the ortho position, more preferably at least one further position, in particular 4 and 6 Or phenyl substituted at adjacent positions, eg, 3,4 or 4,5.
[226] In all compounds, at least one of R ³¹ and R ³⁵ is not hydrogen.
[227] In more preferred compounds, M is C (O) CH ₂ , C (O) NH, -CH = CH-, CH ₂ CH ₂ C (O) (CH) ₂ , CH ₂ CHC (O) CH ₂ , most Preferably it is Formula 7:
[228]
[229] In the above formula,
[230] W is CH ₂ or NH.
[231] M is even more preferably selected from one of the following:
[232]
[233] Wherein R ⁴⁰ is preferably hydrogen, alkyl, alkoxy, alkoxyalkyl, haloalkyl, more preferably lower alkyl, lower alkoxy or halo lower alkyl, more preferably hydrogen or lower alkyl, in particular methyl or ethyl And most preferably hydrogen.
[234] M is most preferably to be.
[235] In a preferred compound, R ³¹ , R ³² , R ³³ , R ³⁴ and R ³⁵ are
[236] (i) each independently lower alkyl, halo lower alkyl, phenyl, alkoxy, lower alkylsulfonylamino lower alkyl, cyano lower alkyl, acetyl, lower alkoxycarbonyl, cyano, OH, acetoxy lower alkyl, hydroxy lower Alkyl, acetoxy lower alkoxy or lower alkoxycarbonyl;
[237] (ii) R ³² and R ³³ or R ³³ and R ^{34 form} an alkylenedioxy, preferably methylenedioxy, the remainder of R ³¹ , R ³² , R ³³ , R ³⁴ and R ³⁵ in (i) As defined.
[238] In a preferred embodiment, R ³¹ , R ³³ , R ³⁵ are other than hydrogen, preferably lower alkyl or lower alkoxy, or R ³¹ or R ³⁵ is other than hydrogen, preferably lower alkyl or lower alkoxy, R ³² and R ³³ or R ³³ and R ³⁴ form methylenedioxy.
[239] In all embodiments, preferred substituents can also be determined with reference to Tables 1A-1R, which describe exemplary compounds. Preferred compounds are those of Tables 1a to 1r with the highest activity and preferred substituents are on the compound with the highest activity.
[240] compoundET _A (μM) ^* ET _B (μM) ^*N- (4-bromo-3-methyl-5-isoxazolyl) -5-bromothiophene-2-sulfonamide0.3142.26 N- (4-bromo-3-methyl-5-isoxazolyl) -5- (2'-thienyl) thiophene-2-sulfonamide5.10.363 N- (4-bromo-3-methyl-5-isoxazolyl) -3-phenoxythiophene-2-sulfonamide0.1033.46 N- (3,4-dimethyl-5-isoxazolyl) benzofuran-2-sulfonamide5.2238.4 N- (3,4-dimethyl-5-isoxazolyl) furan-2-sulfonamide3.13- N- (4-bromo-3-methyl-5-isoxazolyl) -5-phenylfuran-2-sulfonamide0.8572.43 N- (4-bromo-3-methyl-5-isoxazolyl) furan-2-sulfonamide0.7588.1 N- (4-bromo-3-methyl-5-isoxazolyl) -2,5-dimethylfuran-2-sulfonamide0.4636.5 N- (4-bromo-3-methyl-5-isoxazolyl) -5- (phenylthio) furan-2-sulfonamide5.07.0 N- (4-bromo-3-methyl-5-isoxazolyl) -1- (phenyl) pyrrole-2-sulfonamide18.18.7 N- (4-bromo-3-methyl-5-isoxazolyl) -1- (4'-isopropylphenyl) pyrrole-2-sulfonamide11.40.166
[241] compoundET _A (μM) ^* ET _B (μM) ^*N- (4-bromo-3-methyl-5-isoxazolyl) -1- (4'-isopropylphenyl) pyrrole-3-sulfonamide0.8380.211 (4-bromo-3-methyl-5-isoxazolyl) -1- (4'-biphenyl) pyrrole-2-sulfonamide9.177.84 N- (4-bromo-3-methyl-5-isoxazolyl) -2-thiophenesulfonamide0.095 ± 0.0727.7 ± 15.0 N- (4-bromo-5-methyl-3-isoxazolyl) thiophene-2-sulfonamide0.21127.3 N- (4-bromo-3-methyl-5-isoxazolyl) thiophen-3-sulfonamide0.13523.4 5- (3-isoxazolyl) -N- (3-methyl-5-isoxazolyl) -2-thiophenesulfonamide5.66.7 N- (4-bromo-3-methyl-5-isoxazolyl) -5- (2-pyridyl) thiophene-2-sulfonamide3.842.70 N- (4-bromo-3-methyl-5-isoxazolyl) -4,5-dibromothiophene-2-sulfonamide0.2812.58 N- (4-bromo-3-methyl-5-isoxazolyl) -5-chloro-3-methylbenzo [b] thiophene-2-sulfonamide0.961.63 N- (4-Bromo-3-methyl-5-isoxazolyl) -5- (4-chlorobenzamidomethyl) -thiophene-2-sulfonamide0.3112.57 N- (4-bromo-3-methyl-5-isoxazolyl) -4-benzenesulfonylthiophene-2-sulfonamide0.383- 4-Bromo-5-chloro-N- (4-bromo-3-methyl-5-isoxazolyl) -thiophene-2-sulfonamide0.3592.67 N- (4-bromo-3-methyl-5-isoxazolyl) -2,5-dimethylthiophen-3-sulfonamide0.09567.8 N- (4-bromo-3-methyl-5-isoxazolyl) -4,5-dichlorothiophene-2-sulfonamide~ 0.45~ 4.9 N- (4-bromo-3-methyl-5-isoxazolyl) -4-bromo-2,5-dichlorothiophen-3-sulfonamide~ 0.2810.4 N- (4-bromo-3-methyl-5-isoxazolyl) -2,5-dichlorothiophen-3-sulfonamide~ 0.392.62 N- (4-bromo-3-methyl-5-isoxazolyl) -5- {3- [1-methyl-5- (trifluoromethyl) pyrazolyl]} thiophene-2-sulfonamide~ 6.7~ 0.36 N- (4-bromo-3-methyl-5-isoxazolyl) -5-benzenesulfonylthiophene-2-sulfonamide0.5700.333
[242] compoundET _A (μM) ^* ET _B (μM) ^*N- (4-bromo-3-methyl-5-isoxazolyl) -2- (carmethoxy) thiophen-3-sulfonamide0.020898.1 N- (3,4-dimethyl-5-isoxazolyl) -5-phenylthiophen-2-sulfonamide2.551.29 N- (4-bromo-3-methyl-5-isoxazolyl) -2- (N-phenylaminocarbonyl) thiophene-3-sulfonamide0.005418.8 N- (4-bromo-5-methyl-3-isoxazolyl) -2- (N-phenylaminocarbonyl) thiophen-3-sulfonamide-- N- (4-chloro-3-methyl-5-isoxazolyl) -2- (N-phenylaminocarbonyl) thiophen-3-sulfonamide-- N- (3,4-dimethyl-5-isoxazolyl) -2- (carboxyl) thiophene-3-sulfonamide2.64> ~ 100 N- (4-chloro-3-methyl-5-isoxazolyl) -2- (carbomethoxy) thiophen-3-sulfonamide N- (3,4-dimethyl-5-isoxazolyl) -2- (N-phenylaminocarbonyl) thiophene-3-sulfonamide0.0182To 170 N- (3,4-dimethyl-5-isoxazolyl) -2- (carbomethoxy) thiophen-3-sulfonamide0.367- N- (4-bromo-3-methyl-5-isoxazolyl) -2- (carboxyl) thiophene-3-sulfonamide~ 0.6To 67 N- (4-bromo-3-methyl-5-isoxazolyl) -2- [N- (4-methoxyphenyl) aminocarbonyl] -thiophene-3-sulfonamide0.0022.12 N- (4-bromo-3-methyl-5-isoxazolyl) -2- [N- (3-methoxyphenyl) aminocarbonyl] -thiophene-3-sulfonamide0.0035.86 N- (4-bromo-3-methyl-5-isoxazolyl) -2- [N- (2-methoxyphenyl) aminocarbonyl] -thiophene-3-sulfonamide0.011613.2 N- (4-bromo-3-methyl-5-isoxazolyl) -2- (N-benzylaminocarbonyl) thiophene-3-sulfonamide0.01312.7 N- (4-Bromo-3-methyl-5-isoxazolyl) -2- [N- (4-ethylphenyl) aminocarbonyl] -thiophene-3-sulfonamide0.00160.849 N- (4-Bromo-3-methyl-5-isoxazolyl) -2- [N- (4-biphenyl) aminocarbonyl] -thiophene-3-sulfonamide0.03760.912 N- (3,4-dimethyl-5-isoxazolyl) -3-methoxythiophen-2-sulfonamide2.545.5
[243] compoundET _A (μM) ^* ET _B (μM) ^*N- (4-bromo-3-methyl-5-isoxazolyl) -5- (4-ethylphenyl) thiophene-2-sulfonamide3.230.0855 N- (4-bromo-3-methyl-5-isoxazolyl) -3-phenylthiophene-2-sulfonamide0.054711.1 N- (4-bromo-3-methyl-5-isoxazolyl) -4-phenylthiophen-2-sulfonamide0.2241.17 N- (3,4-dimethyl-5-isoxazolyl) benzo [b] thiophene-2-sulfonamide7.2211.1 N- (4-bromo-3-methyl-5-isoxazolyl) -2-phenylthiophen-3-sulfonamide-- N- (4-chloro-3-methyl-5-isoxazolyl) -2- (N-phenylaminocarbonyl) thiophen-3-sulfonamide-- N- (4-bromo-3-methyl-5-isoxazolyl) -5-benzylthiophene-2-sulfonamide-- N- (4-chloro-3-methyl-5-isoxazolyl) -2-carboxythiophene-3-sulfonamide-- N- (4-bromo-3-methyl-5-isoxazolyl) -5- (4'-isopropylphenyl) thiophene-2-sulfonamide1.60.3 N- (4-bromo-3-methyl-5-isoxazolyl) -4- (4'-isopropylphenyl) thiophene-2-sulfonamide5.51.3 N- (4-bromo-3-methyl-5-isoxazolyl) -5- (4'-propylphenyl) thiophene-2-sulfonamide5.60.51 N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[-(4-tolulyl-aminocarbonyl] thiophene-3-sulfonamide<0.01 ^** 1.67 ^**N- (4-bromo-3-methyl-5-isoxazolyl) -2- [N- (4-isopropylphenyl) amino-carbonyl] thiophene-3-sulfonamide<0.01 ^** 1.13 ^**N- (4-Bromo-3-methyl-5-isoxazolyl) -2- (4-t-butylphenyl) aminocarbonyl-thiophene-3-sulfonamide0.011 ^** 2.82 ^**N- (4-bromo-3-methyl-5-isoxazolyl) -2- (4-butylphenyl) aminocarbonylthiophene-3-sulfonamide0.044 ^** 2.84 ^**N- (4-Bromo-3-methyl-5-isoxazolyl) -2- (N- (4-tert-butylphenyl) aminocarbonyl] -thiophene-3-sulfonamide~ 0.008 ^** 1.76 ^**N- (3,4-dimethyl-5-isoxazolyl) -2-methylbenzo [b] thiophene-3-sulfonamide0.16716.6 N- (4-bromo-3-methyl-5-isoxazolyl) -2-methylbenzo [b] thiophene-3-sulfonamide0.04863.5
[244] compoundET _A (μM) ^* ET _B (μm) ^*N- (4-bromo-3-methyl-5-isoxazolyl) -2-ethylbenzo [b] thiophene-3-sulfonamide0.00675.13 N- (4-bromo-3-methyl-5-isoxazolyl) -2-n-benzylbenzo [b] thiophene-3-sulfonamide0.0182To 1 N- (4-bromo-3-methyl-5-isoxazolyl) -2-butylbenzo [b] thiophene-3-sulfonamide0.0226~ 3 N- (4-bromo-3-methyl-5-isoxazolyl) -2-i-propylbenzo [b] thiophene-3-sulfonamide0.0050.03 ^† 5.710.7 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-n-propylbenzo [b] thiophene-3-sulfonamide0.0240.074 ^† 7.96516.6 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2- (4-ethylbenzyl) benzo [b] thiophene-3-sulfonamide0.048 ^† 1.1 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) benzyl] benzo [b] -thiophene-3-sulfonamide0.0015 ± 0.00140.0074 ± 0.0011 ^† 0.324 ± 0.780.939 ± 0.262 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -2- (3,4,5-trimethoxybenzyl) -benzo [b] -thiophene-3-sulfonamide0.013 ^† 1.2 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-ethyl-5-methylbenzo [b] thiophene-3-sulfonamide1.89 ± 0.431 ^† 54.3 ± 2.6 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(3,4-methylenedioxy) benzyl] benzo [b] -thiophene-3-sulfonamide0.011 ± 0.005 ^† 0.936 ± 0.095 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2- (3,4-dimethoxybenzyl) benzo [b] thiophen-3-sulfonamide0.021 ± 0.017 ^† 2.94 ± 1.32 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -5- (benzo [B] thien-2-yl) thiophene-2-sulfonamide16 ^† 0.80 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2- (4-methoxybenzyl) benzo [b] thiophene-3-sulfonamide0.051 ^† 1.5 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2- (2-methoxybenzyl) benzo [b] thiophene-3-sulfonamide0.19 ^† 2.2 ^†N- (3,4-dimethyl-5-isoxazolyl) -2- (4-chlorobenzyl) benzo [b] thiophene-3-sulfonamide0.21 ^† 4.7 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- (4-dimethylaminobenzyl) benzo [b] -thiophene-3-sulfonamide0.041 ^† 0.0141.3 ^† 0.477 N- (4-chloro-3-methyl-5-isoxazolyl) -2-ethylbenzo [b] furan-3-sulfonamide0.15 ^† 22 ^†
[245] compoundET _A (μM) ^* ET _B (μM) ^*N- (4-chloro-3-methyl-5-isoxazolyl) -2-phenylbenzo [b] thiophene sulfonamide0.932 ^† 46.8 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -6-methoxy-2- [3,4- (methylenedioxy) benzyl] -benzo [b] thiophene-3-sulfonamide~ 2 ^{est †} 2.39 ^†N- (4-chloro-5-methyl-3-isoxazolyl) -2- [3,4- (methylenedioxy) benzyl] benzo [b] -thiophene-3-sulfonamide0.0055 ^† 0.364 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2-methoxycarbonylthiophene-3-sulfonamide0.63153.2 N- (4-bromo-3-methyl-5-isoxazolyl) -4- (4-propylphenyl) thiophene-2-sulfonamide0.962 ^† 0.435 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3- (phenylthio) thiophene-2-sulfonamide0.0801 ^† 3.68 ^†N- (3,4-dimethyl-5-isoxazolyl) -3- (phenylaminocarbonyl) thiophene-2-sulfonamide0.163> 100 N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(4-tolyl) aminocarbonyl] thiophen-3-sulfonamide0.001160.0105 ^† 2.9314 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -5- (4-methoxyphenyl) thiophene-2-sulfonamide8.6926.3 ^† 0.3632.4 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -5- (3-methoxyphenyl) thiophene-2-sulfonamide3.2623.4 ^† 0.7764.7 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -5- (3-thienyl) thiophene-2-sulfonamide4.490.380 N- (4-bromo-3-methyl-5-isoxazolyl) -3-methylthiophene-2-sulfonamide0.6517.15 N- (4-bromo-3-methyl-5-isoxazolyl) -3- (phenethyl) thiophene-2-sulfonamide0.160.676 ^† 10.7737.2 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -4- (phenethyl) thiophen-2-sulfonamide6.643.97 N- (3,4-dimethyl-5-isoxazolyl) -2-[(4-methylphenyl) aminocarbonyl] thiophene-3-sulfonamide0.0033611.3 N- (4-bromo-3-methyl-5-isoxazolyl) -2,5-dimethyl-4-phenylthiophen-3-sulfonamide1.40To 100 N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(methyl) phenylaminocarbonyl] thiophene-3-sulfonamide0.18816.0
[246] compoundET _A (μM) ^* ET _B (μM) ^*N- (4-bromo-3-methyl-5-isoxazolyl) -2- (α-hydroxybenzyl) thiophen-3-sulfonamide0.3379.37 N- (4-bromo-5-methyl-3-isoxazolyl) -5- (4-methylphenyl) thiophene-2-sulfonamide7.1015.8 ^† 0.35930.25 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -5-phenylthiophen-2-sulfonamide3.5336.6 ^† 0.4172.4 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -5- [4- (trifluoromethyl) phenyl] -thiophene-2-sulfonamide6.396.31 ^† 0.0835.282 ^†N, N'-bis {3-[(4-bromo-3-methyl-5-isoxazolyl) aminosulfonyl) thien-2-yl} urea0.06920.295 ^† 0.2901.19 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2- (hydroxymethyl) thiophene-3-sulfonamide0.1601.55 ^† 44.1-- N- (4-bromo-3-methyl-5-isoxazolyl) -5- (2-formylphenyl) thiophen-3-sulfonamide3.4612.31 ^† 0.5291.28 ± 0.71 ^†N, N'-bis {3- [3,4-dimethyl-5-isoxazolyl) aminosulfonyl] thien-2-yl} -urea1.01 ± 1.032.7 ^† 3.7 ± 2.75.9 ^†N- (3,4-dimethyl-5-isoxazolyl) -2-[(3-methoxyanilino) methyl] thiophene-3-sulfonamide0.2140.933 ^† 5.347.7 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -5- (3-aminophenyl) thiophene-2-sulfonamide0.5371.44 ^† 1.072.63 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -5- [3,5-bis (trifluoromethyl) -phenyl] thiophene-2-sulfonamide0.7945.9 ^† 12.015.5 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -5- (3,3-dimethylbutyn-1-yl) thiophene-2-sulfonamide1.127.24 ^† 24.035.5 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -5- (2-methoxyphenyl) thiophene-2-sulfonamide0.3811.097 N- (4-bromo-3-methyl-5-isoxazolyl) -5- (2-tolyl) thiophene-2-sulfonamide0.4320.313 N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[(3-carboxyphenyl) -aminocarbonyl] -thiophene-3-sulfonamide0.062 ^† > 100 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[(2-carboxyphenyl) -aminocarbonyl] -thiophene-3-sulfonamide0.21 ^† 20 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2- (aminocarbonyl) thiophen-3-sulfonamide0.84 ^† > 100 ^†
[247] compoundET _A (μM) ⁺ ET _B (μM) ^*N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[(5-dimethylamino-1-naphthyl) -sulfonyl-aminocarbonyl] thiophen-3-sulfonamide0.97 ^† 3.9 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -5- (5-methyl-2-thienyl) thiophene-2-sulfonamide17 ^† 0.21 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(3,4-methylenedioxyphenyl) amino-carbonyl] thiophene-3-sulfonamide0.017 ^† 9.8 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[(3,4-methylenedioxy) phenoxycarbonyl-thiophene-3-sulfonamide0.0073 ^† 6.0 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3-[(3,4-methylenedioxy) phenyl]] thiophene-2-sulfonamide0.50 ^† 79 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3-[(3,4-methylenedioxy) benzyl] thiophene-2-sulfonamide8.1 ^† 3.2 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3-benzylthiophene-2-sulfonamide1.6 ^† 39 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -5- (3-tolyl) thiophene-2-sulfonamide15 ^† 4.2 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(3,4-methylenedioxy) benzyl] thiophene-3-sulfonamide0.27 ^† 7.7 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(3,4-methylenedioxy) benzoyl] thiophene-3-sulfonamide2.0 ^† 15 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[(2-hydroxyphenyl) aminocarbonyl] -thiophene-3-sulfonamide0.013 ^† 38 ^†N- (3,4-dimethyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) phenoxycarbonyl] -thiophene-3-sulfonamide6.1 ^† > ~ 50 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -5- (5-ethylthien-2-yl) thiophene-2-sulfonamide24 ^† 7.7 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[(3,4-methylenedioxy) benzoyl] amino-carbonyl] -thiophene-3-sulfonamide0.089 ^† 37 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2-[(3,4-methylenedioxy)]-phenoxycarbonyl] -thiophene-3-sulfonamide0.0065 ^† 7.4 ^†
[248] compoundET _A (μM) ^* ET _B (μM) ^*N- (4-bromo-3-methyl-5-isoxazolyl) -5- (1-pentynyl) thiophene-2-sulfonamide29 ^† 5.6 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -5- (5-ethylthien-2-yl) thiophen-2-sulfonamide12 ^† 0.71 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[(3,4-methylenedioxy) phenylacetyl] -thiophene-3-sulfonamide0.0091 ^† 5.5 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) phenoxycarbonyl-amino] thiophen-3-sulfonamide0.087 ^† 5.9 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[(2-chloro-3,4-methylenedioxy) -phenoxymethyl] thiophene-3-sulfonamide13 ^† 0.76 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -2- [trans- (3,4-methylenedioxy) -cinnamil] -thiophene-3-sulfonamide0.14 ^† 1.4 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -5- (1-naphthyl) -thiophene-2-sulfonamide14 ^† 1.4 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -5- (3-nitrophenyl) thiophene-2-sulfonamide26 ^† 4.5 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[(3,4-methylenedioxy) phenylureido] -thiophene-3-sulfonamide0.57 ^† 1.3 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2-[(3,4- (methylenedioxy) phenylacetyl] -thiophene-3-sulfonamide0.021 ^† 6.5 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -5- (4-methoxycarbonylphenyl) thiophene-2-sulfonamide> 100 ^† 17 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -5- (4-carboxyphenyl) thiophene-2-sulfonamide> 100 ^† 31 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -5-[(4-tolyl) aminocarbonyl)] thiophene-2-sulfonamide28 ^† 8.6 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -5- (2-methylfuranyl) thiophene-2-sulfonamide32 ^† 7.5 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) benzyloxy-carbonyl] thiophene-3-sulfonamide.42 ^† 12 ^†
[249] compoundET _A (μM) ^* ET _B (μM) ^*N- (4-Chloro-3-methyl-5-isoxazolyl) -2- [2- (3,4-methylenedioxyphenyl)] ethoxy-carbonyl-3-sulfonamide.23 ^† 6.2 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2-{[4- (3,4-methylenedioxybenzyl) -piperazin-1-yl] carbonyl} thiophen-3- Sulfonamide20 ^† > ~ 100 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2-aminothiophen-3-sulfonamide14 ^† 6.2 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -5- (benzyloxymethyl) thiophene-2-sulfonamide12 ^† 9.0 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- {1-cyano-1-[(3,4-methylenedioxy) -phenyl] acetyl} thiophene-3-sulfonamide2.1 ^† 27 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(3,4-methylenedioxy) phenethyl] thiophen-3-sulfonamide0.21 ^† 9.2 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2-[(3-dimethylamino) phenoxycarbonyl] -thiophene-3-sulfonamide1.4 ^† 60 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -1-methylindole-2-sulfonamide77 ^† ~ 100 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- (cyclohexyloxycarbonyl) thiophen-3-sulfonamide0.44 ^† 34 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2- [β-hydroxy (3,4-methylenedioxy) -phenylethyl] thiophene-3-sulfonamide0.053 ^† 16 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-carboxyl-1-methylindole-3-sulfonamide0.59 ^† 104 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2-[(4-oxacyclohexyl) oxycarbonyl] -thiophene-3-sulfonamide1.37 ^† _ N-2- [3,4- (methylenedioxy) phenylacetyl] thiophene-3-sulfonamide1.8 ^† 32.5 ^†N- (4-Chloro-3-methyl-5-isoxazolyl)-{2- [3,4- (methylenedioxy) phenylacetyl} -thiophene-3-sulfonamide oxime-- N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(4-tolyl) aminocarbonyl] -1-methylindole-3-sulfonamide31.3 ^† 14.7 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(4-methoxyphenoxy) carbonyl] thiophene-3-sulfonamide0.023 ^† 15 ^†
[250] compoundET _A (μM) ^* ET _B (μM) ^*N- (4-bromo-3-methyl-5-isoxazolyl) -1- [3,4- (methylenedioxy) benzyl] indole-2-sulfonamide5.29 ^† 18.6 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(4-methylphenoxy) carbonyl] thiophene-3-sulfonamide122 ^† 9.7 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(4-methoxyphenyl) acetyl] thiophen-3-sulfonamide0.043 ^† 10.1 ^†N- (4-bromo-3-methyl-5-isoxazolyl-3-[(4-methylphenoxy) methyl] thiophene-2-sulfonamide1.64 ^† 22.8 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(4-methylphenoxy) methyl] thiophene-3-sulfonamide1.2 ^† 15 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-methyl-trans-styryl) thiophene-2-sulfonamide0.94 ^† 0.66 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-methylphenethyl) thiophene-2-sulfonamide0.347 ^† 9.4 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(4-methylphenyl) acetyl] thiophen-3-sulfonamide0.198 ^† 9.13 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(3-methoxyphenyl) acetyl] thiophen-3-sulfonamide0.030 ^† 19.1 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -3- (4-methylphenethyl) -5- (4-tolyl) -thiophene-2-sulfonamide6.1 ^† 2.09 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-methylbenzyl) -5- (4-tolyl) thiophen-2-sulfonamide4.69 ^† 1.56 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -3- (4-methyl-trans-styryl) -5- (4-tolyl) thiophene-2-sulfonamide6.9 ^† 1.58 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- [β, β- (ethylenedioxy) -3,4- (methylenedioxy) phenethyl] thiophen-3-sulfonamide0.128 ^† 2.09 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2- [β- (dimethylamino) -3,4- (methylene-dioxy) phenethyl] thiophene-3-sulfonamide20.9 ^† ~ 100 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2- {α-hydroxy- [3,4- (methylene-dioxy) phenyl] acetyl} thiophene-3-sulfonamide2.5 ^† 30 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2-[(5-methyl-3-isoxazolyl) amino-carbonyl] thiophene-3-sulfonamide0.056 ^† 92 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[(3-hydroxy-6-pyridazinyl) -aminocarbonyl] thiophene-3-sulfonamide0.066 ^† 81.3 ^†
[251] compoundET _A (μM) ^* ET _B (μM) ^*N- (4-chloro-3-methyl-5-isoxazolyl) 2-{[2-acetyl-4,5- (methylenedioxy) -phenyl] aminocarbonyl} thiophene-3-sulfonamide0.010 ^† 31.6 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3-{[3,4- (methylenedioxy) phenoxy] -methyl} thiophene-2-sulfonamide0.513 ^† 9.6 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(4-methyl) (cinnamil)] thiophene-3-sulfonamide0.26 ^† 0.413 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(4,5-dimethoxy-2-methoxycarbonyl-phenyl) aminocarbonyl] thiophen-3-sulfonamide0.55 ^† - N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(2-methyl-1,3,4-thiadiazol-5-yl) aminocarbonyl] thiophen-3-sulfone amides0.13 ^† - N- (4-chloro-3-methyl-5-isoxazolyl) -2-{[4,5-dimethoxy-2,4,5-dimethoxy-2-methoxycarbonyl) phenyl] phenylaminocarbon Nyl} thiophene-3-sulfonamide3.80 ^† - N- (4-chloro-3-methyl-5-isoxazolyl) -2-{[2-carboxy-4,5- (methylenedioxy) -phenyl] aminocarbonyl} thiophene-3-sulfonamide1.43 ^† - N- (4-bromo-3-methyl-5-isoxazolyl) -3- [3,4- (methylenedioxy) phenethyl] thiophene-2-sulfonamide0.236 ^† 18 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -3- [3,4- (methylenedioxy) -trans-styryl] thiophene-2-sulfonamide0.218 ^† 10 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(4-methyl) phenethyl] thiophen-3-sulfonamide0.106 ^† 40.1 ^†N- (3,4-dimethyl-5-isoxazolyl) -2-{[2-acetyl-4,5- (methylenedioxy) phenyl] -aminocarbonyl} thiophene-3-sulfonamide0.032 ^† - N- (4-chloro-3-methyl-5-isoxazolyl) -2- [4-methoxy-2-methylphenyl) amino-carbonyl] thiophen-3-sulfonamide0.027 ^† 0.14 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2-[{2-cyano-4,5-dimethoxyphenyl) -aminocarbonyl] thiophene-3-sulfonamide0.0039 ^† 12.2 ^†N- (3,4-dimethyl-5-isoxazolyl) -2- (4-tolylacetylphenyl) -thiophene-3-sulfonamide0.0027 ^† 29.2 ^†
[252] compoundET _A (μM) ^* ET _B (μM) ^*N- (3,4-dimethyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) phenylacetyl] thiophene-3-sulfonamide0.0273 ^† 12.2 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(2,4-dimethoxyphenyl) amino-carbonyl] thiophen-3-sulfonamide0.158 ^† 63.1 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2-[(3-methyl-6-pyridyl) aminocarbonyl] -thiophene-3-sulfonamide0.023 ^† 43.7 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2-[(2-hydroxy-4-methylphenyl) amino-carbonyl] thiophene-3-sulfonamide.006 ^† - N- (4-chloro-3-methyl-5-isoxazolyl) -2-{[2-cyano-4,5- (methylenedioxy) -phenyl] aminocarbonyl} thiophene-3-sulfonamide0.0034 ^† 40.4 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2- [2-methyl-4,5- (methylenedioxy) phenyl-aminocarbonyl] thiophene-3-sulfonamide0.0030 ^† 355 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2- (2-carboxyxamido-4,5-dimethoxyphenyl-aminocarbonyl) thiophene-3-sulfonamide0.011 ^† 61 ^†N- (3,4-dimethyl-5-isoxazolyl) -2- (2,4-dimethylphenylacetyl) thiophene-3-sulfonamide0.0027 ^† 17.4 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,4-dimethylphenylacetyl) thiophene-3-sulfonamide0.0004 ^† 4.8 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2- (2,4-dimethylphenylacetyl) thiophene-3-sulfonamide0.0008 ^{† **} 3.6 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy)] phenylamino-carbonyl-3-thiophenesulfonamide0.0073 ^† 9.2 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- [2-methyl-4,5- (methylenedioxy) phenyl-acetyl] thiophen-3-sulfonamide0.0032 ^† 9 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6- (2-acetoxyethyl) phenylaminocarbonyl] thiophen-3- Sulfonamide0.0045 ^† 25.7 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6- (2-hydroxyethyl) phenylaminocarbonyl] thiophen-3- Sulfonamide0.0056 ^† 16.8 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3,5-dimethylphenylacetyl) thiophene-3-sulfonamide0.045 ^† 17.7 ^†
[253] compoundET _A (μM) ^* ET _B (μM) ^*N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,5-dimethylphenylacetyl) thiophene-3-sulfonamide0.007 ^† 18 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- [2-methanesulfonylaminomethyl) -4,5- (methylenedioxy) phenylaminocarbonyl] thiophene-3-sulfone amides0.0068 ^† 19.8 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2- [2-cyanomethyl-4,5- (methylene-dioxy) -6-cyanomethyl] -phenylaminocarbonyl- 3-thiophenesulfonamide0.0038 ^† 25 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- [2-hydroxyproyl-4,5- (methylenedioxy) phenylaminocarbonyl] thiophen-3-sulfonamide0.0073 ^† 8.3 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -3- [2-methyl-4,5- (methylenedioxy) -cinnamil] thiophene-2-sulfonamide~ 0.1 ^{† **} ~ 6 ^{† **}N- (4-Bromo-3-methyl-5-isoxazolyl) -3- [2-methyl-4,5- (methylenedioxy) -phenethyl] thiophene-2-sulfonamide~ 0.1 ^{† **} ~ 5 ^{† **}N- (4-bromo-3-methyl-5-isoxazolyl) -3-{[2-propyl-4,5- (methylenedioxy) -phenoxy] methyl} thiophene-2-sulfonamide~ 0.2 ^{† **} ~ 1.5 ^{† **}N- (4-Chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6- (2-acetoxyethoxy)] phenylaminocarbonyl] thiophene- 3-sulfonamide~ 0.02 ^{† **} ~ 18 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6- (2-hydroxyethoxy) phenylaminocarbonyl] thiophen-3 Sulfonamides~ 0.01 ^{† **} ~ 18 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2- [2-cyano-4,5- (methylenedioxy) -phenylacetyl] thiophene-3-sulfonamide~ 0.3 ^{† **} ~ 0.7 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- {2-[(dimethylamino) carbonylmethyl] -4,5- (methylenedioxy) phenylaminocarbonyl} thiophene- 3-sulfonamide0.009 ^† 13.8 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2- [2-methyl-4,5- (methylenedioxy) -phenylhydroxyamino] thiophene-3-sulfonamide0.794 ^† 6.49 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -2- [2-methyl-4,5- (methylenedioxy) -phenethyl] thiophene-3-sulfonamide0.0619 ^† 8.90 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3- [2- (hydroxymethyl) -4,5- (methylenedioxy) cinnamyl] thiophene-2-sulfonamide0.0795 ^† 3.24 ^†
[254] compoundET _A (μM) ^* ET _B (μM) ^*N- (4-bromo-3-methyl-5-isoxazolyl) -3- {2-[(tetrahydro-4H-pyran-2-yloxy) methyl] -4,5- (methylenedioxy) Cinnamic} thiophene-2-sulfonamide0.0967 ^† 4.14 N- (4-bromo-3-methyl-5-isoxazolyl) -3- (2,4-dimethylphenethyl) thiophene-2-sulfonamide0.1006 ^† 4.30 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3- (2,4-dimethylcinnamil) thiophene-2-sulfonamide0.180 ^† 2.97 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2- (2,4-dimethylcinnamil) thiophene-3-sulfonamide0.166 ^† 2.97 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -3-[(2,4-dimethylphenoxy) methyl] -thiophene-2-sulfonamide0.346 ^† 7.45 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[(2,4-dimethylphenoxy) methyl] -thiophene-3-sulfonamide0.308 ^† 4.48 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -5- (phenylaminocarbonyl) thiophene-2-sulfonamide28.1 ^† 60.6 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- [β-acetoxy-2-methyl-4,5- (methylenedioxy)) styryl] thiophene-3-sulfonamide0.005443.74 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2-[(2,3,4-trimethoxy-6-cyano) -phenylaminocarbonyl] thiophene-3-sulfonamide0.000169 ^† 12.5 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- [2- (cyano) phenyl] benzo [b] thiophene-3-sulfonamide6.33 ^† 8.82 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) phenyl] benzo- [b] thiophene-3-sulfonamide0.550 ^† 52.6 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3- (2-tolyl) thiophene-2-sulfonamide0.324 ^† 55.1 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3- (3-tolyl) thiophene-2-sulfonamide0.832 ^† 21.2 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3- (2-tolyl) thiophene-2-sulfonamide0.302 ^† 31% @ 100 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3- (3-methoxyphenyl) thiophene-2-sulfonamide0.334 ^† ** N- (4-bromo-3-methyl-5-isoxazolyl) -3- (3-methoxyphenyl) thiophene-2-sulfonamide1.32 ^† 56.3 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3- (2-methoxyphenyl) thiophene-2-sulfonamide1.71 ^† 59.1 ^†
[255] compoundET _A (μM) ^* ET _B (μM) ^*N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-ethylphenyl) thiophene-2-sulfonamide0.18443.9 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-propylphenyl) thiophene-2-sulfonamide0.08738.48 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-iso-propylphenyl) thiophene-2-sulfonamide0.21828.3 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-butylphenyl) thiophene-2-sulfonamide0.1606.11 ^†N- (3,4-dimethyl-5-isoxazolyl) -2- [2-methyl-4,5- (methylenedioxy) phenyl-acetyl] thiophen-3-sulfonamide0.00328 ^† 34.3 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,4,6-trimethylphenylamino-carbonyl) thiophen-3-sulfonamide0.000626 ^† 8.27 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2- (2,4,6-trimethylphenylacetyl) -thiophene-3-sulfonamide0.000238 ^† 3.82 ^†N- (4-Chloro-5-methyl-3-isoxazolyl) -2- [2-methyl-4,5- (methylenedioxy) -phenylacetyl] thiophene-3-sulfonamide0.000625 ^† 3.69 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -2- [2-methyl-4,5- (methylenedioxy) -cinnamil] thiophene-3-sulfonamide0.0804 ^† 3.28 ^†N- (4-bromo-methyl-5-isoxazolyl) -2- (2,4-dimethylphenethyl) thiophen-3-sulfonamide0.0555 ^† 3.48 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2-[(4-methoxycarbonyl-2,6-dimethyl) -phenylaminocarbonyl] thiophene-3-sulfonamide0.000266 ^† 9.78 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- (phenoxycarbonyl) thiophen-3-sulfonamide4.41 ^† 31% @ 100 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2- (phenoxycarbonyl) thiophen-3-sulfonamide2.71 ^† 20% @ 100 ^†N- (3,4-dimethyl-5-isoxazolyl) -2-{[3,4- (methylenedioxy) phenoxy] carbonyl} -thiophene-3-sulfonamide3.61 ^† 30% @ 100 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(2-methylphenoxy) carbonyl] thiophene-3-sulfonamide0.684 ^† 105 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(3-methylphenoxy) carbonyl] thiophene-3-sulfonamide1.20 ^† 111 ^†
[256] compoundET _A (μM) ^* ET _B (μM) ^*N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[(2,4-dimethylphenoxy) carbonyl] -thiophene-3-sulfonamide0.291 ^† 43.2 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(2-methoxyphenoxy) carbonyl] thiophen-3-sulfonamide0.761 ^† 29% @ 100 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(3-methoxyphenoxy) carbonyl] thiophene-3-sulfonamide0.79 ^† 90 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(4-methoxyphenoxy) carbonyl] thiophen-3-sulfonamide1.73 ^† 111 ^†N- (3,4-dimethyl-5-isoxazolyl) -2-[(4-methoxyphenoxy) carbonyl] thiophene-3-sulfonamide5.88 ^† 13% @ 100 ^†N- (3,4-dimethyl-5-isoxazolyl) -2-[(4-methoxyphenoxy) carbonyl] thiophene-3-sulfonamide2.5 ^† 33% @ 100 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(4-methylphenoxy) carbonyl] thiophene-3-sulfonamide3.2 ^† 43% @ 100 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2-[(2,4-dimethylphenoxy) carbonyl] -thiophene-3-sulfonamide0.648 ^† 68.5 ^†N- (3,4-dimethyl-5-isoxazolyl) -2-[(2,4-dimethylphenoxy) carbonyl] thiophene-3-sulfonamide0.274 ^† 21% @ 100 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -2-{[2-propyl-4,5- (methylenedioxy) -phenoxy] carbonyl} thiophene-3-sulfonamide0.138 ^† 11.9 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-methoxycarbonyl-2,4,6-trimethyl-phenylaminocarbonyl) thiophene-3-sulfonamide0.000321 ^† 0.00092 ^† 16.5 ^† - N- (4-bromo-3-methyl-5-isoxazolyl) -3- (2,4-dimethylphenyl) thiophene-2-sulfonamide0.100 ^† 60.3 ^†N- (3,4-dimethyl-5-isoxazolyl) -2- (phenoxycarbonyl) thiophen-3-sulfonamide2.85 ^† 31% ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-iso-butylphenyl) thiophene-2-sulfonamide0.0823 ^† 2.76 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-iso-pentylphenyl) thiophene-2-sulfonamide0.155 ^† 3.31 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -3-[(2,4,6-trimethylphenoxy) methyl] -thiophene-2-sulfonamide0.0457 ^† 4.68 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[(2,4,6-trimethylphenoxy) methyl] -thiophene-3-sulfonamide0.0562 ^† 3.39 ^†
[257] compoundET _A (μM) ^* ET _B (μM) ^*N- (4-bromo-3-methyl-5-isoxazolyl) -3- (2,4,6-trimethylcinnamil) thiophene-2-sulfonamide0.0490 ^† 1.86 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3- (2-methyl-4-propylphenyl) thiophene-2-sulfonamide0.0468 ^† 3.63 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -3- (4-iso-butyl-2-methylphenyl) -thiophene-2-sulfonamide0.0468 ^† 1.66 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -3- (4-iso-pentyl-2-methylphenyl) -thiophene-2-sulfonamide0.107 ^† 2.40 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-{[3,4- (methylenedioxy) phenoxy] -methyl} thiophene-3-sulfonamide0.302 ^† 6.61 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2-{[4,5- (methylenedioxy) -2-propylphenoxy] methyl} thiophene-3-sulfonamide0.107 ^† 0.407 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2- (2,4,6-trimethylphenethyl} thiophene-3-sulfonamide0.0417 ^† 1.23 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -3- (2,4,6-trimethylphenethyl) -thiophene-2-sulfonamide0.055 ^† 1.62 ^†N- (3,4-dimethyl-5-isoxazolyl) -2-[(2,4,6-trimethylphenoxy) carbonyl] -thiophene-3-sulfonamide0.537 ^† 8% @ 100 ^†N- (4-Chloro-3-methyl-5-isoxazolyl) -2-[(2,4,6-trimethylphenoxy) -carbonyl] thiophene-3-sulfonamide0.0776 ^† 30.2 ^†N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[(2,4,6-trimethylphenoxy) -carbonyl] thiophene-3-sulfonamide0.479 ^† 24.5 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-cyanomethyl-2,4,6-trimethylphenylaminocarbonyl) thiophene-3-sulfonamide0.0006 ^† ~ 45 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-carboxymethyl-2,4,6-trimethylphenylaminocarbonyl) thiophene-3-sulfonamide0.0015 ^† ~> 100 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-acetoxymethyl-2,4,6-trimethylphenylaminocarbonyl) thiophene-3-sulfonamide0.0006 ^† >> 100 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-hydroxymethyl-2,4,6-trimethylphenylaminocarbonyl) thiophene-3-sulfonamide0.0004 ^† ~ 80 ^{† *} In general, 2 to 5 times the average of the results ^** least one data point of the experiment is only a rough measure the preliminary results or results in black ^† performed by incubation at 24 ℃. As described in the Examples, the culture at higher temperatures reduces the activity by 2 to about 10 times compared to the activity at 4 ° C .-- Data% inhibition rate which cannot be obtained or measured as inhibition rate @ 100 μM% @ 100μM%
[258] It is of course possible to replace the 4-bromo or 4-chloro groups with other 4-halo substituents or other suitable substituents for R ¹ , for example alkyl, in particular alkyl having 1 to 15 carbons in the chain.
[259] 2. Ar ² is a substituted 4-biphenyl group.
[260] Provided is a compound of Formula 1, wherein Ar ¹ is N- (5-isoxazolyl) or N- (3-isoxazolyl) and Ar ² is selected from biphenyl derivatives. These compounds may be represented by the following formula (8a) or (8b).
[261]
[262]
[263] In the above formula,
[264] R ²⁶ and R ¹³ are each independently H, OH, HONH, NH ₂ , NO ₂ , halide, pseudohalide, alkyl, alkenyl, alkynyl, aryl, arylalkyl, heteroaryl, alkoxy, alkylamino, dialkylamino , Alkylthio, haloalkoxy, haloalkyl, alkylsulfinyl, alkylsulfonyl, aryloxy, arylamino, arylthio, arylsulfinyl, arylsulfonyl, haloalkyl, haloaryl, alkoxycarbonyl, carbonyl, alkylcarbon Nilyl, aminocarbonyl, arylcarbonyl, formyl, substituted or unsubstituted amido, substituted or unsubstituted ureido, wherein the alkyl, alkenyl and alkynyl moieties are from about 1 to about 14 carbons Atoms, preferably containing 1 to 6 carbon atoms, are straight or branched or cyclic, and the aryl moiety contains about 4 to about 16 carbons, preferably 4 to 10 carbons. R ¹³ and R ²⁶ are preferably selected from H, lower alkyl, haloalkyl and halide, respectively. In addition, Ar ² may each independently be substituted with one or more substituents selected from the selections described for R ²⁶ and R ¹³ , wherein R ² and R ¹ are as defined above.
[265] In embodiments herein, the biphenylsulfonamide is substituted 4-biphenylsulfonamide, R ¹³ is preferably present in the para position and R ²⁶ is present in any position except the 2-position when not hydrogen. .
[266] In a more preferred embodiment, R ¹ is halide or methyl or higher (C ₉ -C ₁₃ ) alkyl. R ¹ is selected from halides, CH ₃ , C ₂ H ₅ , CF ₃ , C ₂ F ₅ , nC ₃ H ₇ and cyclo-C ₃ H ₇ , preferably halides or CH ₃ , R ² is H, CH ₃ , C ₂ H ₅ , CF ₃ , C ₂ F ₅ , nC ₃ H ₇ and cyclo-C ₃ H ₇ , more preferably R ¹ is halide or CH ₃ , and R ² is H, CH ₃ , C ₂ H ₅ or CF ₃ .
[267] In a more preferred embodiment, R ¹ is Cl or Br, or higher alkyl (C ₉ C ₁₉ to C ₁₃ H ₂₇ ) if greater ET _B activity is desired; R ² is selected from H, CH ₃ , C ₂ H ₅ , CF ₃ , C ₂ F ₅ , nC ₃ H ₇ , cyclo-C ₃ H ₇ , nC ₁₃ H ₂₇ and nC ₉ H ₁₉ . In even more preferred embodiments, R ¹ is Br, Cl or C ₉ H ₁₉ to C ₁₃ H ₂₇ ; R ² is H, CH ₃ , C ₂ H ₅ or CF ₃ .
[268] Biphenyl compounds provided herein are generally ET _B activity or ET _B selectivity (see, eg, Table 2); That is, the compounds provided herein inhibit binding of the endotelin to the ET _B receptor at a concentration about 10 to 30 times lower than inhibiting binding to the ET _A receptor. In particular, 4-biphenylsulfonamide is ET _B selective.
[269] In general, in all of the embodiments herein, 4-haloisoxazole sulfonamide is ET _A when compared to the corresponding sulfonamide where the substituent at position 4 in isoxazolyl is other than halo, eg, alkyl. And / or exhibit substantially enhanced activity (about 2 to 20 fold higher activity) with respect to one or more ET receptors, as analyzed by assays as provided herein, which measure binding to the ET _B receptor. For example, the IC ₅₀ for the ET _A receptor of N- (3,4-dimethyl-5-isoxazolyl) -2-biphenylsulfonamide is about 0.008 μM, whereas N- (4-bromo-3 The IC ₅₀ of -methyl-5-isoxazolyl) -2-biphenylsulfonamide is about 0.0016 μM (see Table 2 below); (3) the IC ₅₀ for the ET _B receptor of N- (3,4-dimethyl-5-isoxazolyl) -3-biphenylsulfonamide is about 3.48 μM; The IC ₅₀ for N- (4-bromo-3-methyl-5-isoxazolyl) -3-biphenylsulfonamide for the ET _B receptor is about 0.76 μM and N- (4-chloro-3-methyl-5 The IC ₅₀ for -isoxazolyl) -3-biphenylsulfonamide for the ET _B receptor is about 0.793 μΜ (see Table 2 below).
[270] Exemplary biphenyl sulfonamides include, but are not limited to, the following compounds and those listed in Table 2:
[271] N- (3-methyl-5-isoxazolyl) -4'-methylphenyl-4-biphenylsulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -4'-methylphenyl- 4-biphenylsulfonamide, N- (4-chloro-3-methyl-5-isoxazolyl) -4'-methylphenyl-4-biphenylsulfonamide, (3-methyl-5-isoxazolyl) -4 '-Trifluorophenyl-4-biphenylsulfonamide, (4-bromo-3-methyl-5-isoxazolyl) -4'-trifluorophenyl-4-biphenylsulfonamide, (3-methyl -5-isoxazolyl) -4'-methoxyphenyl-4-biphenylsulfonamide, (4-bromo-3-methyl-5-isoxazolyl) -4'-methoxyphenyl-4-biphenyl Sulfonamide, (4-bromo-3-methyl-5-isoxazolyl) -3'-methoxyphenyl-4-biphenylsulfonamide, (4-bromo-3-methyl-5-isoxazolyl) -2'-methoxyphenyl-4-biphenylsulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3 ', 4'-methylenedioxyphenyl-4-biphenylsulfon Amide and (4-bromo-3-methyl-5-isoxazolyl) -3'-methylphenyl-4-biphenylsulfonamide. Corresponding 4-chloro and 4-fluoro isoxazolyl compounds are also included herein.
[272] Exemplary biphenyl compounds tested using the exemplified assays (see Examples) and provided for illustrative purposes or for comparison with the compounds provided herein, but are not limited thereto, are shown in the following table (Table 2) do:
[273] compoundET _A (μM) ^* ET _B (μM) ^*N- (4-bromo-3-methyl-5-isoxazolyl) -4-biphenylsulfonamide3.349 ^† ~ 0.171.23 ^†N- (4-bromo-5-methyl-3-isoxazolyl) -4-biphenylsulfonamide6.4 ± 249 ^† 0.29 ± 0.021.78 ^†N- (4-chloro-3-methyl-5-isoxazolyl) -4-biphenylsulfonamide4.93 ± 30.29 ± 0.1 N- (3,4-dimethyl-5-isoxazolyl) -4-biphenylsulfonamide9.9 ± 1.46.3 ^† 0.77 ± 0.320.15 ^†N- (4-chloro-5-methyl-3-isoxazolyl) -4-biphenylsulfonamide3.718.6 ^† 0.23 ± 0.011.29 ^†N- (4-methyl-3-trifluoromethyl-5-isoxazolyl) -4-biphenylsulfonamide19.0--1.75.62 ^†N- (4-tridecyl-3-trifluoromethyl-5-isoxazolyl) -4-biphenylsulfonamide34.0 ± 933.0 ^† 0.99 ± 0.20.95 ^†N- (3,4-dimethyl-5-isoxazolyl) -2-biphenylsulfonamide0.0083 ± 0.001412.8 N- (4-bromo-3-methyl-5-isoxazolyl) -2-biphenylsulfonamide0.00127 ^** 8.54 ^**N- (4-chloro-3-methyl-5-isoxazolyl) -2-biphenylsulfonamide0.00123 ^** ~ 14 ^**N- (3,4-dimethyl-5-isoxazolyl) -3-biphenylsulfonamide> 0.03 ^** 3.48 ^**N- (4-bromo-3-methyl-5-isoxazolyl) -3-biphenylsulfonamide~ 0.03 ^** 0.76 ^**N- (4-chloro-3-methyl-5-isoxazolyl) -3-biphenylsulfonamide> 0.03 ^** 0.793 ^**N- (4-bromo-3-methyl-5-isoxazolyl) -4'-methylphenyl-4-biphenylsulfonamide14.53 ± 9.622.17 ± 3.77 ^* 0.046 ± 0.0440.168 ± 0.0032 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -4'-trifluorophenyl-4-biphenylsulfonamide5.4 ± 0.325.9 ± 13.7 ^† 0.083 ± 0.020.71 ± 0.43 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -4'-methoxyphenyl-4-biphenylsulfonamide14.7 ± 5.6121.5 ± 2.12 ^† 1.15 ± 0.443.94 ± 0.89 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3'-methylphenyl-4-biphenylsulfonamide4.97 ± 3.4162.6 ± 7.14 ^† 0.66 ± 0.252.08 ± 0.23 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -2'-methoxyphenyl-4-biphenylsulfonamide3.3 ± 3.50.41 ± 0.14 N- (4-bromo-3-methyl-5-isoxazolyl) -3 ', 4'-methylenedioxyphenyl-4-biphenylsulfonamide38.2 ± 4.95 ^† 3.0 ± 0.78 ^†N- (4-bromo-3-methyl-5-isoxazolyl) -3'-methylphenyl-4-biphenylsulfonamide--^* Results from 1, 2 or 3 experiments in general using the same formulation ^** preliminary results
[274] Preferred compounds are 4-biphenyl, wherein Ar ² is of formula 7, and at least one substituent R ¹³ is in the para position. Preferred substituents are lower alkyl, halo lower alkyl and lower alkoxy. This compound is ET _B activity.
[275] The preparation of the above-mentioned compounds and other compounds having the essential activity is described in the Examples.
[276] B. Sulfonamides and Sulfonamide Derivatives
[277] Also provided are sulfonamides and sulfonamide derivatives. These compounds are active in assays measuring endothelin antagonist activity. Sulfonamides have the general formula
[278] Formula 1
[279]
[280] In the above formula,
[281] Ar ¹ is isoxazole;
[282] Ar ² is an expression ego;
[283] R ³ and R ⁴ are hydrogen or substituents that are considered to provide enhanced resistance of the compound (ie, by changing the pharmacodynamic profile of the compound). Such substituents are preferably independently selected from halo, cyano, cyanoalkyl, C (O) R ⁴¹ , alkyl, alkenyl, cycloalkyl and aryl, or R ³ and R ⁴ together form alkylene; W is O, NH or CH ₂ ;
[284] R ⁵ , R ⁶ and R ⁷ are each independently,
[285] (i) R ⁶ is hydrogen, unsubstituted alkyl, hydroxy, unsubstituted alkoxy, C (O) R ⁴¹ , carbamoyloxy or alkoxycarbonyloxy, and R ⁵ and R ⁷ are each independently hydrogen, unsubstituted Ring alkyl, hydroxy, C (O) R ⁴¹ , carbamoyloxy and alkoxycarbonyloxy;
[286] (ii) when at least one of R ³ and R ⁴ is not hydrogen, two of them may form alkylenedioxy, the remainder as defined in (i);
[287] R ⁴⁵ is alkyl, C (O) R ⁴¹ , (CH ₂ ) _x OH and CH (OH) (CH ₂ ) _x CH ₃ (where x is 0 to 6), S (O) _n R ⁴¹ , wherein , n is 0 to 2) and C (= NR ⁴³ ) R ⁴¹ ; R ⁴¹ is alkyl, aryl, heteroaryl, aralkyl, heteroaralkyl, cycloalkyl, alkylamino, dialkylamino, arylamino, diarylamino, (aryl) (alkyl) amino, alkylsulfonylamino, arylsulfonyl Amino, (alkylsulfonyl) (alkyl or aryl) amino or (arylsulfonyl) (alkyl or aryl) amino; R ⁴³ is selected from hydroxy, alkoxy, alkyl and aryl. R ⁴¹ and R ⁴³ are selected from Y defined as alkoxy, halide, pseudohalide, alkylcarbonyl, arylcarbonyl, amino, alkylamino, dialkylamino, carboxyl, alkoxylcarbonyl, aryloxycarbonyl or hydroxy It is either unsubstituted or substituted with one or more substituents. Also of interest are corresponding 3-acyl-2-thiophenesulfonamides.
[288] These compounds appear to avoid the toxicological effects associated with hydrophobicity while exhibiting good efficacy, potency, bioavailability, in vivo half-life and / or stability when compared to compounds in which the aryl group has two or more hydrogen substituents. Table 4). In addition, these compounds appear to exhibit good profiles in standard in vitro toxicity tests.
[289] It has now been found that for in vivo administration, it is desirable to achieve a suitable degree of hydrophobicity that reduces the potential hemolytic properties of the compounds. In the present invention, for example, this is achieved when the aryl group is tetra-, o- or hexsubstituted, preferably missubstituted. When the aryl group is tetrasubstituted, it is preferably achieved when substituted at the 2, 4 and 6 positions and when one of the substituents is a polar group such as hydroxyl, acetoxy, carboxyl and carboxamide Turned out. Such substitutions enhance the endothelin antagonist activity and hydrophilicity of the compound. If an aryl group is substituted at the 2, 4 and 6 positions with a nonpolar group, for example an alkyl group, more specifically a methyl group, the aryl group will preferably be o- or hexsubstituted. In the missubstituted aryl group, the fifth substituent is in the 3 position and will preferably be a polar group such as hydroxyl, acetoxy, carboxyl and carboxamide. Such substitutions are desirable to achieve the highest level of activity for therapeutic use.
[290] Such substitutions provide the compound with good bioavailability, long in vivo half-life, and / or good in vivo efficacy. In view of the technology herein, other optimal substituent patterns and substituents can be determined using experimentally suitable animal models.
[291] As noted above, the compounds of this embodiment provide enhanced resistance compared to similar compounds known in the art. This enhanced resistance is evidenced by changes in the pharmacodynamic profile of the compound. Pharmacodynamic profiles are based on a number of factors, including but not limited to bioavailability, in vivo half-life, in vivo efficacy, efficacy, stability, receptor-selectivity, and the like.
[292] In certain embodiments, the compound is hydrogen or one of R ⁵ , R ⁶ and R ⁷ is hydrogen and the compound is N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,4,6- Trimethylphenylaminocarbonyl) thiophene-3-sulfonamide, N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,4,6-trimethyl) phenylacetyl-3-thiophenyl Sulfonamide, N- (3,4-dimethyl-5-isoxazolyl) -2-[(2,4,6-trimethylphenoxy) carbonyl] thiophene-3-sulfonamide, N- (4-chloro -3-methyl-5-isoxazolyl) -2-[(2,4,6-trimethylphenoxy) carbonyl] thiophene-3-sulfonamide or N- (4-bromo-3-methyl-5 -Isoxazolyl) -2-[(2,4,6-trimethylphenoxy) carbonyl] thiophene-3-sulfonamide.
[293] In another embodiment, the sulfonamide is a compound wherein the compound is N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-methoxy-2,4,6-trimethylphenylaminocarbonyl) thiophene -3-sulfonamide, N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-hydroxy-2,4,6-trimethylphenylaminocarbonyl) thiophene-3-sulfone Amide, N- (4-chloro-5-methyl-3-isoxazolyl) -2- (3-hydroxy-2,4,6-trimethylphenylaminocarbonyl) thiophene-3-sulfonamide or N- Selected as an additional condition that is not (3,4-dimethyl-5-isoxazolyl) -2- (3-hydroxy-2,4,6-trimethylphenylaminocarbonyl) thiophene-3-sulfonamide do.
[294] Preferred compounds are those in which R ³ and R ⁴ are each independently hydrogen, alkyl, halo, cyano, cyanomethyl, acetyl or cycloalkyl or together form alkylene;
[295] R ⁵ , R ⁶ and R ⁷ are each independently,
[296] (i) R ⁶ is hydrogen, unsubstituted alkyl, hydroxy, unsubstituted alkoxy, C (O) R ⁴¹ , carbamoyloxy or alkoxycarbonyloxy, and R ⁵ and R ⁷ are each independently hydrogen, non- Ring alkyl, hydroxy, C (O) R ⁴¹ , carbamoyloxy or alkoxycarbonyloxy;
[297] (ii) when at least one of R ³ and R ⁴ is not hydrogen, two of them may form alkylenedioxy, and the other is as defined in (i);
[298] Provided that at least one of R ⁵ , R ⁶ and R ⁷ is hydrogen;
[299] x is 0 or 1; n is 2; R ⁴¹ is alkyl, cycloalkyl, alkylamino, dialkylamino, arylamino, diarylamino, alkylsulfonylamino or arylsulfonylamino; R ⁴³ is hydroxy or alkoxy, provided that N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,4,6-trimethylphenylaminocarbonyl) thiophen-3-sulfone Amide, N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,4,6-trimethyl) phenylacetyl-3-thiophenylsulfonamide, N- (3,4-dimethyl- 5-isoxazolyl) -2-[(2,4,6-trimethylphenoxy) carbonyl] thiophene-3-sulfonamide, N- (4-chloro-3-methyl-5-isoxazolyl)- 2-[(2,4,6-trimethylphenoxy) carbonyl] thiophen-3-sulfonamide or N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(2, Compounds other than 4,6-trimethylphenoxy) carbonyl] thiophen-3-sulfonamide.
[300] In particular, preferred compounds are those wherein Ar ¹ is 4-chloro-3-methyl-5-isoxazolyl; R ³ and R ⁴ are each independently hydrogen, methyl, cyclopropyl, fluoro, chloro, cyano, cyanomethyl or acetyl or together form butylene;
[301] R ⁵ , R ⁶ and R ⁷ are each independently,
[302] (i) R ⁶ is selected from hydrogen, methyl, hydroxy, methoxy, acetyl, carbamoyloxy or methoxycarbonyloxy, and R ⁵ and R ⁷ are each independently hydrogen, methyl, hydroxy, acetyl, carba Moyloxy and methoxycarbonyloxy;
[303] (ii) when at least one of R ³ and R ⁴ is not hydrogen, R ⁶ and R ⁷ may form methylenedioxy, and R ⁵ is as defined in (i);
[304] Provided that at least one of R ⁵ , R ⁶ and R ⁷ is hydrogen;
[305] R ⁴⁵ is acetyl, propanoyl, 2-methylpropanoyl, cyclopropylcarbonyl, benzoyl, cyclohexylcarbonyl, methyl, 1-hydroxy-1-ethyl, hydroxymethyl, methoxyacetyl, fluoroacetyl , Carboxyacetyl, hydroxyacetyl, oxyminoacetyl or SO ₂ R ⁴¹ , provided that N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,4,6-trimethylphenylamino Carbonyl) thiophene-3-sulfonamide, N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,4,6-trimethyl) phenylacetyl-3-thiophenylsulfonamide, N- (3,4-dimethyl-5-isoxazolyl) -2-[(2,4,6-trimethylphenoxy) carbonyl] thiophene-3-sulfonamide, N- (4-chloro-3- Methyl-5-isoxazolyl) -2-[(2,4,6-trimethylphenoxy) carbonyl] thiophen-3-sulfonamide and N- (4-bromo-3-methyl-5-isox Compounds other than zolyl) -2-[(2,4,6-trimethylphenoxy) carbonyl] thiophene-3-sulfonamide.
[306] Particularly preferred compounds are selected from the following compounds:
[307] N- (2-acetyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thiophencarboxamide,
[308] N- (2-benzoyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thiophencarboxamide,
[309] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2-hydroxyethaneimidoyl-4,6-dimethylphenyl) -2-thiophencarbox amides,
[310] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-propionylphenyl) -2-thiophencarboxamide,
[311] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2-isobutyryl-4,6-dimethylphenyl) -2-thiophencarboxamide,
[312] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (cyclohexylcarbonyl) -4,6-dimethylphenyl) -2-thiophenka Voxamide,
[313] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (cyclopropylcarbonyl) -4,6-dimethylphenyl) -2-thiophenka Voxamide,
[314] 3-(((3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thienyl) carbonyl) amino) -2,4,6-trimethylphenyl carba Mate,
[315] 3-(((3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thienyl) carbonyl) amino) -2,4,6-trimethylphenyl methyl Carbonate,
[316] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2- (3-methoxy-2,4,6-trimethylphenyl) acetyl) -3-thiophenesulfonamide,
[317] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2- (3-hydroxy-2,4,6-trimethylphenyl) acetyl) -3-thiophenesulfonamide,
[318] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2-hydroxy-1-methylethyl) -4,6-dimethylphenyl)- 2-thiophencarboxamide,
[319] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2-hydroxyethyl) -4,6-dimethylphenyl) -2-thiophene Carboxamide,
[320] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2,6-diacetyl) -4-methylphenyl) -2-thiophencarbox amides,
[321] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2-methoxyacetyl) -4,6-dimethylphenyl) -2-thiophene Carboxamide,
[322] 3- (2-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thienyl) carbonyl) amino) -3,5-dimethylphenyl) -3-oxo Propane Acid,
[323] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2-glycolyl-4,6-dimethylphenyl) -2-thiophencarboxamide,
[324] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-methylsulfonyl) phenyl) -2-thiophenecarboxamide ,
[325] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-((methylamino) sulfonyl) phenyl) -2-ti Offencarboxamide,
[326] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-((dimethylamino) sulfonyl) phenyl) -2-ti Offencarboxamide,
[327] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-(((methylsulfonyl) amino) carbonyl) phenyl) 2-thiophenecarboxamide,
[328] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2-fluoroacetyl) -4,6-dimethylphenyl) -2-thiophene Carboxamide,
[329] N- (2-acetyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -5-methyl-2-thiophencarbox amides,
[330] N- (2-benzoyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -5-methyl-2-thiophencarbox amides,
[331] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2-hydroxyethaneimidoyl) -4,6-dimethylphenyl) -5-methyl-2 -Thiophene carboxamide,
[332] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-propionylphenyl) -5-methyl-2-thiophenka Voxamide,
[333] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2-isobutyryl-4,6-dimethylphenyl) -5-methyl-2-thiophene Carboxamide,
[334] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (cyclohexylcarbonyl) -4,6-dimethylphenyl) -5-methyl-2 -Thiophene carboxamide,
[335] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (cyclopropylcarbonyl) -4,6-dimethylphenyl) -5-methyl-2 -Thiophene carboxamide,
[336] 3-(((3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2- (5-methylthienyl)) carbonyl) amino) -2,4, 6-trimethylphenyl carbamate,
[337] 3-(((3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2- (5-methylthienyl)) carbonyl) amino) -2,4, 6-trimethylphenyl methyl carbonate,
[338] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2- (3-methoxy-2,4,6-trimethylphenyl) acetyl) -5-methyl-3-thiophenesulfone amides,
[339] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2- (3-hydroxy-2,4,6-trimethylphenyl) acetyl) -5-methyl-3-thiophenesulfone amides,
[340] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2-hydroxy-1-methylethyl) -4,6-dimethylphenyl)- 5-methyl-2-thiophencarboxamide,
[341] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2-hydroxyethyl) -4,6-dimethylphenyl) -5-methyl- 2-thiophencarboxamide,
[342] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2,6-diacetyl) -4-methylphenyl) -5-methyl-2- Thiophencarboxamide,
[343] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2-methoxyacetyl) -4,6-dimethylphenyl) -5-methyl- 2-thiophencarboxamide,
[344] 3- (2-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2- (5-methylthienyl)) carbonyl) amino) -3,5-dimethylphenyl ) -3-oxopropanoic acid,
[345] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2-glycolyl-4,6-dimethylphenyl) -5-methyl-2-thiophenka Voxamide,
[346] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-methylsulfonyl) phenyl) -5-methyl-2-ti Offencarboxamide,
[347] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-((methylamino) sulfonyl) phenyl) -5-methyl 2-thiophenecarboxamide,
[348] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-((dimethylamino) sulfonyl) phenyl) -5-methyl 2-thiophenecarboxamide,
[349] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-(((methylsulfonyl) amino) carbonyl) phenyl) -5-methyl-2-thiophencarboxamide,
[350] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2-fluoroacetyl) -4,6-dimethylphenyl) -5-methyl- 2-thiophencarboxamide,
[351] N- (6-acetyl-4-methyl-1,3-benzodioxol-5-yl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -5 -Methyl-2-thiophencarboxamide,
[352] N- (4-acetyl-6-methyl-1,3-benzodioxol-5-yl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -5 -Methyl-2-thiophencarboxamide,
[353] N- (2-acetyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -4-methyl-2-thiophencarbox amides,
[354] N- (2-benzoyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -4-methyl-2-thiophencarbox amides,
[355] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2-hydroxyethaneimidoyl) -4,6-dimethylphenyl) -4-methyl-2 -Thiophene carboxamide,
[356] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-propionylphenyl) -4-methyl-2-thiophenka Voxamide,
[357] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2-isobutyryl-4,6-dimethylphenyl) -4-methyl-2-thiophene Carboxamide,
[358] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (cyclohexylcarbonyl) -4,6-dimethylphenyl) -4-methyl-2 -Thiophene carboxamide,
[359] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (cyclopropylcarbonyl) -4,6-dimethylphenyl) -4-methyl-2 -Thiophene carboxamide,
[360] 3-(((3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2- (4-methylthienyl)) carbonyl) amino) -2,4, 6-trimethylphenyl carbamate,
[361] 3-(((3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2- (4-methylthienyl)) carbonyl) amino) -2,4, 6-trimethylphenyl methyl carbonate,
[362] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2- (3-methoxy-2,4,6-trimethylphenyl) acetyl) -4-methyl-3-thiophenesulfone amides,
[363] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2- (3-hydroxy-2,4,6-trimethylphenyl) acetyl) -4-methyl-3-thiophenesulfone amides,
[364] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2-hydroxy-1-methylethyl) -4,6-dimethylphenyl)- 4-methyl-2-thiophencarboxamide,
[365] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2-hydroxyethyl) -4,6-dimethylphenyl) -4-methyl- 2-thiophencarboxamide,
[366] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2,6-diacetyl) -4-methylphenyl) -4-methyl-2- Thiophencarboxamide,
[367] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2-methoxyacetyl) -4,6-dimethylphenyl) -4-methyl- 2-thiophencarboxamide,
[368] 3- (2-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2- (4-methylthienyl)) carbonyl) amino) -3,5-dimethylphenyl ) -3-oxopropanoic acid,
[369] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2-glycolyl-4,6-dimethylphenyl) -4-methyl-2-thiophenka Voxamide,
[370] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-methylsulfonyl) phenyl) -4-methyl-2-ti Offencarboxamide,
[371] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-((methylamino) sulfonyl) phenyl) -4-methyl 2-thiophenecarboxamide,
[372] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-((dimethylamino) sulfonyl) phenyl) -4-methyl 2-thiophenecarboxamide,
[373] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-(((methylsulfonyl) amino) carbonyl) phenyl) -4-methyl-2-thiophencarboxamide,
[374] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2-fluoroacetyl) -4,6-dimethylphenyl) -4-methyl- 2-thiophencarboxamide,
[375] N- (6-acetyl-4-methyl-1,3-benzodioxol-5-yl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -4 -Methyl-2-thiophencarboxamide,
[376] N- (4-acetyl-6-methyl-1,3-benzodioxol-5-yl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -4 -Methyl-2-thiophencarboxamide,
[377] N- (2-acetyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -4,5-dimethyl-2-thiophene Carboxamide,
[378] N- (2-benzoyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -4,5-dimethyl-2-thiophene Carboxamide,
[379] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2-hydroxyethaneimidoyl-4,6-dimethylphenyl) -4,5-dimethyl- 2-thiophencarboxamide,
[380] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-propionylphenyl) -4,5-dimethyl-2-ti Offencarboxamide,
[381] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2-isobutyryl-4,6-dimethylphenyl) -4,5-dimethyl-2- Thiophencarboxamide,
[382] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (cyclohexylcarbonyl) -4,6-dimethylphenyl) -4,5-dimethyl 2-thiophenecarboxamide,
[383] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (cyclopropylcarbonyl) -4,6-dimethylphenyl) -4,5-dimethyl 2-thiophenecarboxamide,
[384] 3-(((3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2- (4,5-dimethylthienyl)) carbonyl) amino) -2, 4,6-trimethylphenyl carbamate,
[385] 3-(((3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2- (4,5-dimethylthienyl)) carbonyl) amino) -2, 4,6-trimethylphenyl methyl carbonate,
[386] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2- (3-methoxy-2,4,6-trimethylphenyl) acetyl) -4,5-dimethyl-3-t Offensulfonamide,
[387] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2- (3-hydroxy-2,4,6-trimethylphenyl) acetyl) -4,5-dimethyl-3-t Offensulfonamide,
[388] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2-hydroxy-1-methylethyl) -4,6-dimethylphenyl)- 4,5-dimethyl-2-thiophencarboxamide,
[389] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2-hydroxyethyl) -4,6-dimethylphenyl) -4,5- Dimethyl-2-thiophencarboxamide,
[390] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2,6-diacetyl) -4-methylphenyl) -4,5-dimethyl- 2-thiophencarboxamide,
[391] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2-methoxyacetyl) -4,6-dimethylphenyl) -4,5- Dimethyl-2-thiophencarboxamide,
[392] 3- (2-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2- (4,5-dimethylthienyl)) carbonyl) amino) -3,5- Dimethylphenyl) -3-oxopropanoic acid,
[393] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2-glycolyl-4,6-dimethylphenyl) -4,5-dimethyl-2-ti Offencarboxamide,
[394] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-methylsulfonyl) phenyl) -4,5-dimethyl-2 -Thiophene carboxamide,
[395] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-((methylamino) sulfonyl) phenyl) -4,5 Dimethyl-2-thiophencarboxamide,
[396] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-((dimethylamino) sulfonyl) phenyl) -4,5 Dimethyl-2-thiophencarboxamide,
[397] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-(((methylsulfonyl) amino) carbonyl) phenyl) -4,5-dimethyl-2-thiophencarboxamide,
[398] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (2-fluoroacetyl) -4,6-dimethylphenyl) -4,5- Dimethyl-2-thiophencarboxamide,
[399] N- (6-acetyl-4-methyl-1,3-benzodioxol-5-yl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -4 , 5-dimethyl-2-thiophencarboxamide and
[400] N- (4-acetyl-6-methyl-1,3-benzodioxol-5-yl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -4 , 5-dimethyl-2-thiophencarboxamide.
[401] In addition, preferred compounds are selected from the following compounds:
[402] N- (2-acetyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -5-cyclopropyl-2-thiophenka Voxamide,
[403] 5-acetyl- (N- (2-acetyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thiophenka Voxamide,
[404] N- (2-acetyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -5-cyano-2-thiophenka Voxamide,
[405] N- (2-acetyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -5- (cyanomethyl) -2- Thiophencarboxamide,
[406] N- (2-acetyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -5-fluoro-2-thiophenka Voxamide,
[407] N- (2-acetyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -5-chloro-2-thiophenecarbox Amides and
[408] N- (2-acetyl-4,6-dimethylphenyl) -5-chloro-3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -4,5,6, 7-tetrahydro-1-benzothiophene-2-carboxamide.
[409] Preferred compounds are those wherein W is O or CH ₂ . Thus, sulfonamides are 2-phenoxycarbonyl-3-sulfonamides, 3-phenoxycarbonyl-2-sulfonamides, 2-phenylacetyl-3-sulfonamides and 3-phenylacetyl-2-sulfones of the compounds. Amide derivatives.
[410] Table 3 describes exemplary compounds of this embodiment and demonstrates that the compounds have activity as endothelin receptor antagonists. More preferred compounds in Table 3 are those with the highest activity and preferred substituents are on the compound with the highest activity. The data in Table 3 is for illustration and comparison, and is not intended to limit the scope of the embodiments in any form.
[411] compoundET _A (μM) ^* ET _A / ET _BN- (2-acetyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) -amino) sulfonyl) -2-thiophencarboxamide0.00055 ^† 34000 ^∥3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2-isobutyryl-4,6-dimethylphenyl) -2-thiophencarboxamide0.00111 ^† 14000 ^∥N- (2-benzoyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) -amino) sulfonyl) -2-thiophencarboxamide0.00426 ^† 6000 ^∥N- (2-acetyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) -amino) sulfonyl) -5-methyl-2-thiophenka Voxamide0.00294 ^† 9000 ^∥3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-propionylphenyl) -2-thiophencarboxamide0.00061 ^† 21000 ^∥3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (cyclo-propylcarbonyl) -4,6-dimethylphenyl) -2-thiophene Carboxamide0.00036 ^† 45000 ^∥3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (cyclo-hexylcarbonyl) -4,6-dimethylphenyl) -2-thiophene Carboxamide0.0149 ^† 1300 ^∥3-(((3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thienyl) -carbonyl) amino) -2,4,6-trimethylphenyl Methyl carbonate0.00075 ^† - 3-(((3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thienyl) -carbonyl) amino) -2,4,6-trimethylphenyl Carbamate0.00545 ^† - Results usually averaged 2 to 5 experiments † Assays performed by incubation at 24 ° C. Carried out as described in the example, a temperature higher than then eseoui culture is reduced to the second activity by about 10 times compared to the activity at 4 ℃ .-- can be obtained or measured as% inhibition @ 100μM data ∥ ET _A / ET _B selectivity
[412] Table 4 lists the oral half-life, bioavailability and in vivo activity of selected exemplary compounds. In vivo activity is measured in a pulmonary circulatory boost model and is a measure of the activity of a compound at a selected dose. As set forth in Table 4, the compounds claimed herein exhibit improved oral half-life, bioavailability and in vivo activity than previously described (see, eg, PCT International Application WO96 / 31492).
[413] compoundP _app ^a POt _1/2 in rat ^b Highest plasma level ^c In vivo efficacy ^dN- (4-chloro-3-methyl-5-isoxazolyl) -2- [2-methyl-4,5- (methylenedioxy) phenylacetyl] thiophen-3-sulfonamide2.324.1173++ N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(2,3,4-trimethoxy-6-cyano) phenylaminocarbonyl] thiophen-3-sulfonamide0.58 N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-cyano-methyl-2,4,6-trimethylphenylaminocarbonyl) thiophene-3-sulfonamide1.783.440.2++ N- (4-Chloro-3-methyl-5-isoxazolyl) -2- (3-carboxy-methyl-2,4,6-trimethylphenylaminocarbonyl) thiophene-3-sulfonamide1.10 N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-hydroxy-methyl-2,4,6-trimethylphenylaminocarbonyl) thiophene-3-sulfonamide1.561.53-/ + N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-methanesulfonyl-amino-2,4,6-trimethylphenylaminocarbonyl) thiophen-3-sulfonamide 5.92.6N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-cyano-2,4,6-trimethylphenylaminocarbonyl) thiophen-3-sulfonamide 3.920++ N- (2-acetyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thiophencarboxamide40%8.657++ ^e3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2-isobutyryl-4,6-dimethylphenyl) -2-thiophencarboxamide 5.4593-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (cyclopropylcarbonyl) -4,6-dimethylphenyl) -2-thiophene- Carboxamide 5.553 ^a × 10 ⁶ cm / sec ^{b In} units of time ^c μg / mL in units ^d pulmonary circulatory boost model: ++ Valid at 5 mg / kg-Invalid at 5 mg / kg + Valid at 15 mg / kg ^e 0.3 mg / kg in vivo Valid at
[414] C. Preparation of Compound
[415] The preparation of neutral (ie, free) sulfonamide compounds having the necessary activity is described in U.S. Pat. US 08 / 721,183 and 08 / 847,797, and co-owned PCT International Applications WO 96/31492 and WO 97/27979. Representative synthetic methods are described in the Examples. Compounds whose synthetic methods are not specifically illustrated herein or in the patents and published international PCT applications listed above may be prepared by replacing them with suitable, available reagents in conventional variations of one or more of the methods detailed in the Examples herein. have.
[416] Salts, acids and other derivatives thereof can be synthesized as summarized and illustrated herein or by other methods known to those skilled in the art.
[417] 1. Neutral Compound
[418] In general, most synthetic methods involve condensation of anhydrous pyridine or tetrahydrofuran (THF) with sulfonyl chloride and aminoisoxazole in sodium hydride. Sulfonyl chlorides and aminoisoxazoles are commercially available, or may be prepared according to the methods described in the examples or using other methods available to those skilled in the art. See, eg, US Pat. 4,659,369, 4,861,366 and 4,753,672].
[419] N- (alkylisoxazolyl) sulfonamides can be prepared by condensing aminoisoxazoles with or without the catalyst 4- (dimethylamino) pyridine in sulfonyl chloride and anhydrous pyridine. N- (3,4-dimethyl-5-isoxazolyl) sulfonamide and N- (4,5-dimethyl-3-isoxazolyl) sulfonamide are equivalent aminodimethylisoxazoles, such as 5- It can be prepared from amino-3,4-dimethylisoxazole. For example, N- (3,4-dimethyl-5-isoxazolyl) -2- (carmethoxy) thiophen-3-sulfonamide is 2-methoxycarbonylthiophen-3-sulfur in anhydrous pyridine. It can be prepared from polyyl chloride and 5-amino-3,4-dimethylisoxazole.
[420] N- (4-haloisoxazolyl) sulfonamide can be prepared by condensing amino-4-haloisoxazole and sulfonyl chloride in THF using sodium hydride as the base. For example, N- (4-bromo-3-methyl-5-isoxazolyl) thiophene-2-sulfonamide is 5-amino-4-bromo-3-methylisoxazole and thiophen-2 It can be prepared in THF and sodium hydride from sulfonyl chloride. N- (4-bromo-3-methyl-5-isoxazolyl) -5- (3-isoxazolyl) thiophene-2-sulfonamide is 5-amino-4-bromo-3-methylisox It can be prepared from solazole and 5- (3-isoxazolyl) thiophene-2-sulfonyl chloride.
[421] In addition, compounds, such as those herein wherein Ar ² is thienyl, furyl and pyrrolyl, may contain suitable sulfonyl chlorides in a solution of 3 and 4 in a tetrahydrofuran (THF) solution containing a base such as sodium hydride. It can be prepared by reacting with a 5-aminoisoxazole substituted at the position, for example, 5-amino-4-bromo-3-methylisoxazole. Following the reaction, THF is removed under reduced pressure, and the residue is dissolved in water, acidified and extracted with methylene chloride. The organic layer is washed, then dried over anhydrous magnesium sulphate, the solvent is evaporated and the residue is purified by recrystallization with hexane / ethyl acetate to give the pure product.
[422] Such sulfonamides can also be prepared from the corresponding sulfonyl chlorides and aminoisoxazoles in the presence or absence of catalytic amounts of 4-dimethylaminopyridine (DMAP) in pyridine. If desired, bis-sulfonyl compounds are obtained as the main product or sole product. Bis-sulfonated products can be readily hydrolyzed to sulfonamide generally at room temperature using aqueous sodium hydroxide and a suitable co-solvent such as methanol or tetrahydrofuran.
[423] Other embodiments include the following:
[424] (a) N- (4-bromo-3-methyl-5-isoxazolyl) -2- (N-phenylaminocarbonyl) thiophene-3-sulfonamide with N- (4-bromo-3- Prepared from methyl-5-isoxazolyl) -2-carboxythiophen-3-sulfonamide, aniline and 1-ethyl-3 '-[3-dimethylaminopropyl] carbodiimide (EDCI). N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(4-methoxyphenyl) aminocarbonyl] thiophene-3-sulfonamide to 4-methoxyaniline, N, N Prepare from '-diisopropylethylamine and N- (4-bromo-3-methyl-5-isoxazolyl) -2-carboxythiophen-3-sulfonamide. N- (4-bromo-3-methyl-5-isoxazolyl) -2- (benzylaminocarbonyl) thiophene-3-sulfonamide to N- (4-bromo-3-methyl-5-isox Prepared from Sazolyl) -2-carboxythiophen-3-sulfonamide and benzylamine as described above.
[425] N- (4-bromo-3-methyl-5-isoxazolyl) -2-carboxythiophene-3-sulfonamide to 5-amino-4-bromo-3-methylisoxazole and 2- (carbo From N- (4-bromo-3-methyl-5-isoxazolyl) -2- (carmethoxy) thiophen-3-sulfonamide prepared from the condensation of methoxy) thiophen-3-sulfonyl chloride Manufacture.
[426] (b) N- (4-bromo-3-methyl-5-isoxazolyl) -1- (4'-isopropylphenyl) pyrrole-2-sulfonamide and N- (4-bromo-3-methyl -5-isoxazolyl) -1- (4'-isopropylphenyl) pyrrole-3-sulfonamide was replaced with 5-amino-4-bromo-3-methylisoxazole and 1- (4'-isopropylphenyl ) Is prepared from a mixture of pyrrole-2-sulfonyl chloride and 1- (4'-isopropylphenyl) pyrrole-3-sulfonyl chloride. Such sulfonyl chlorides are prepared from 1- (4'-isopropylphenyl) pyrrole-2-sulfonic acid, phosphorus oxychloride and phosphorus pentachloride. 1- (4'-isopropylphenyl) pyrrole-2-sulfonic acid is prepared from 1- (4'-isopropylphenyl) pyrrole and chlorosulfonic acid. 1- (4'-isopropylphenyl) pyrrole is prepared from 4-isopropylaniline and 2,5-dimethoxytetrahydrofuran.
[427] 2. Salts of Neutral Compounds
[428] Pharmaceutically acceptable salts of the compounds can be prepared by the methods illustrated or by methods other than those known to those skilled in the art. As illustrated herein, in the case of organic salts, organic bases such as N, N'-dibenzylethylenediamine, chloroprocaine, choline, ammonia, diethanolamine and other hydroxyalkylamines, ethylenediamine , N-methylglucamine, procaine, N-benzylphenethylamine, 1-para-chlorobenzyl-2-pyrrolidin-1'-ylmethylbenzimidazole, diethylamine and other alkylamines, piperazine Or tris (hydroxymethyl) aminomethane can be mixed with an equimolar amount of sulfonamide. Recovery of subsequent salts by chromatography, including but not limited to crystallization, precipitation, concentration of solution, lyophilization, spray drying, normal and reverse phase chromatography or resin chromatography, or other methods known to those skilled in the art. It is possible to provide the desired salt. Pharmaceutically acceptable cationic salts can be prepared by reacting the acid form with a suitable base.
[429] 3. Salts of Hydrophobic Sulfonamides
[430] Provided are methods for preparing alkali metal salts of hydrophobic sulfonamides that are modulators of endothelin activity. More particularly, a method for preparing such sodium salts of sulfonamides is provided. Each method comprises dissolving free sulfonamide in an organic solvent in the presence of a saturated aqueous solution of an alkali metal salt, and recovering and purifying the sulfonamide salt.
[431] Sulfonamides that are converted to alkali metal salts can be prepared by methods well known in the art (see, eg, US Pat. Nos. 5,591,761, 5,594,021). By way of example, N ² -methoxy-N ² -methyl-3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophencarboxamide is substituted with 6-methylbenzo [d] [ 1,3] dioxylyl-5-methyl magnesium chloride is reacted in an organic solvent to give 4-chloro-3-methyl-5- (2- (2- (6-methylbenzo [d] [1,3] dioxol -5-yl) acetyl) -5-yl) acetyl) -3-thienylsulfonamido) isoxazole is obtained as a crude product which is purified by preparative HPLC.
[432] Method of Making Alkali Metal Salts
[433] Another method of preparing the salts is provided herein and illustrated below (Example 7). Briefly, the method comprises the steps of: (a) mixing 5-chloromethyl-6-methylbenzo [d] [1,3] dioxole and activated magnesium in tetrahydrofuran to produce a Grignard reagent; (b) adding N ² -methoxy-N ² -methyl-3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophencarboxamide to the reaction mixture; (c) diluting the mixture from step (b) in turn with a concentrated inorganic acid and an organic solvent to form an aqueous layer and an organic layer; And (d) drying the organic layer and evaporating the solvent to form a residue.
[434] The salt-forming method starts with dissolving the free sulfonamide in an organic solvent. Suitable organic solvents for use in this method are well known in the art. Exemplary preferred organic solvents are ethyl acetate, methyl t-butyl ether, methylene chloride, THF, ether, acetonitrile, dioxane and chloroform. Ethyl acetate is the most preferred organic solvent.
[435] The formation of alkali metal salts proceeds by exposing an organic solvent containing free sulfonamide to a saturated solution of alkali metal salts. The particular salt used depends on the sulfonamide salt desired to be formed. Suitable alkali metals for use in the process are well known in the art and include sodium, potassium, calcium, magnesium and the like. For the preparation of sulfonamide salts useful in pharmaceutical compositions, sodium and calcium are preferred alkali metals. Sodium is most preferred. Anionic components of salts are well known in the art and include carbonates, phosphates, bicarbonates, nitrates, hydroxides and the like and combinations thereof. Carbonates, bicarbonates and hydroxide anions are preferred. Bicarbonates are more preferred. Alkali metals used to form sulfonamide salts are in the form of highly concentrated aqueous solutions. Preference is given to using saturated solutions. Methods of preparing saturated alkali metal salt solutions are known in the art. The biphasic mixture is stirred by a number of methods including shaking, stirring, sonication and the like. The layers are separated and then the aqueous phase is removed.
[436] Recovery of the product from the organic solvent is carried out using methods well known in the art, for example crystallization and filtration. In one embodiment, the organic solvent containing the sulfonamide salt is washed with a concentrated salt solution, wherein the alkali metal is the same as used for salt formation. If the alkali metal salt is sodium, exemplary washings are concentrated sodium chloride solution (eg saline) or sodium bicarbonate solution. Once the protonated form of sulfonamide is converted to the salt form, it is important to use concentrated (greater than about 3% by weight) salt washes. Surprisingly, alkali metal sulfonamide salts are more soluble in organic solvents than in saturated alkali metal solutions. The use of salt diluents for washing organic solvents (eg semi-concentrated saline) or water can result in disproportionation of the product between water and the organic layer, and subsequent loss of material. After washing, the product solution is concentrated to dryness to give the crude product, for example as a residue. In a preferred embodiment, drying is carried out in Na ₂ SO ₄ or MgSO ₄ and concentration is carried out in vacuo.
[437] In addition, the residue is recovered and purified using recrystallization. According to this embodiment, the product is dissolved in an organic, water-immiscible solvent. Such solvents are well known in the art. Exemplary and preferred such solvents are ethers and halomethanes such as dichloromethane and chloroform. Combinations of these solvents may also be used. The crystalline product can be separated from the organic solvent through filtration. The recovered product can be washed one or more times with an organic, water-immiscible solvent. 4-Chloro-3-methyl-5- (2- (2- (6-methylbenzo [d] [1,3] dioxol-5-yl) acetyl) -3-thienylsulfonamim according to the described method Detailed description of the process for the preparation of isoxazoles, sodium salts can be found in the Examples herein below.
[438] The sulfonamide salts provided herein can be converted back to the free sulfonamide form and purified also by the above method. Sulfonamide salts are dissolved in an aqueous solvent such as water and filtered. Preferably, the filtration is carried out through at least one layer of filter paper. Negative pressure or suction may not be required for complete filtration. In some cases, large amounts (10% or more) of impurities that do not dissolve in water delay the filtration process. This problem can be avoided by using filter paper of larger size in filtration. Usually, when the purity of the crude salt is 90% or more, there is no problem regarding filtration.
[439] Separate salts, typically in the form of turbid solutions, are converted to acids by exposing the salts to concentrated inorganic acids. Suitable acids include hydrochloric acid (HCl), sulfuric acid (H ₂ SO ₄ ), nitric acid (H ₂ NO ₃ ), and the like. Acidification is continued until the pH of the product solution is about 1.5 to about 2.5. Acidification is preferably carried out at temperatures below about 10 ° C. During acidification the product precipitates out as a milky non-filtration material. The acid is slowly added dropwise to some excess to form a fine, easily filterable precipitate. The precipitate is filtered off, washed with water until neutral and pressed on the filter to remove excess water. The free acid obtained typically has a purity of greater than 95% as determined by HPLC. The purified sulfonamide can then be converted to an alkali metal salt by the procedure described above.
[440] The execution of the methods provided herein can reduce the reaction time and it is possible to obtain pure products than other methods. Direct separation of sulfonamide salts can be carried out by mixing the product with a concentrated alkaline salt solution and an organic solvent. A surprising important observation is that sulfonamide salts remain in the organic layer as long as the aqueous layer is darker than expected. This allows for direct separation of the salts and can be further purified by converting these salts into free sulfonamides, converting them back to salts and recrystallizing. This finding is key to large scale synthesis of high purity sulfonamide salts.
[441] 4. Other derivatives
[442] Prodrugs and other derivatives of compounds suitable for human administration can also be designed and prepared by methods known to those skilled in the art. See, eg, Nogrady (1985) Medicinal Chemistry A Biochemical Approach , Oxford University. Press, New York, pages 388-392.
[443] The compounds described herein are synthesized and tested for activity in in vitro assays and, if desired, in vivo animal models. Nuclear magnetic resonance spectroscopy (NMR), mass spectroscopy, infrared spectroscopy, and high performance liquid chromatography analysis indicate that the compound has a structure consistent with that expected for such a compound, and generally has a purity of at least about 98%. All compounds exemplified or described herein exhibit activity as endothelin antibiotics.
[444] D. Formulation of Sulfonamide and Sulfonamide Salts
[445] Provided herein are formulations of such sulfonamides and sulfonamide salts. Formulations prepared as described below are compositions designed for the administration of pharmaceutically acceptable derivatives, in particular the salts of the sulfonamide compounds provided herein. Due to the observed excellent stability properties of the salts, compared to the neutral form, these salts, in particular the sodium salts, are particularly suitable for oral and parenteral administration. Such compositions include solvents, suspensions, tablets, dispersible tablets, pills, capsules, powders, anhydrous powders for inhalers, sustained release formulations and other suitable formulations. Preferably the composition takes the form of pills or tablets. Tablets, capsules and other methods of making such formulations are known to those skilled in the art. See, eg, Ansel, HC (1985) Intooduction to Pharmaceutical Dosage Forms , 4th Edition, pp. 126-163].
[446] Of course, in the formulations herein, derivatives of such compounds, such as pharmaceutically acceptable acids, esters, salts and prodrugs, are of course preferred. Sodium salts, in particular sodium salts where Na ⁺ is a counterion, are preferably used herein to prepare the formulation.
[447] In the formulation, an effective concentration of one or more pharmaceutically acceptable derivatives is mixed with a pharmaceutically acceptable carrier or vehicle. Preferably, the sulfonamide compound is derivatized as a corresponding salt, preferably sodium salt, prior to formulation as described above. Concentrations of the salts of the compounds in the formulations are effective for delivery, in administration, in amounts that relieve the symptoms of endothelin-mediated disease. Typically, the composition is formulated in a single dose administration. To formulate the composition, the mass fraction of the compound is dissolved, suspended, dispersed or otherwise mixed in a selected vehicle at a concentration effective to alleviate or alleviate the condition to be treated.
[448] Pharmaceutical carriers or vehicles suitable for the administration of the compounds provided herein include carriers known to those skilled in the art that are suitable for the particular mode of administration. In addition, the compounds may be formulated as the only pharmaceutically active ingredient in the compound or combined with other active ingredients. Liposomal suspensions comprising tissue-targeted liposomes may also be suitable as pharmaceutically acceptable carriers. These can be prepared by methods known to those skilled in the art. For example, liposome formulations can be prepared as described in US Pat. No. 4,522,811.
[449] The active compound as a salt, preferably a sodium salt, is included in a pharmaceutically acceptable carrier in an amount sufficient to exert a therapeutically effective effect without adverse effects on the patient to be treated. Therapeutically effective concentrations are known from known in vitro and in vivo systems [US Pat. No. 5,114,918, Ishikawa et al. ; EP A1 0 436 189, BANYU PHARMACEUTICAL CO., LTD (7 October 1991); Borges et al. (1989) Eur. J. Pharm. 165 : 223-230; Filep et al. (1991) Biochem. Biophys. Res. Commun. 177 : 171-176, which can be used to make experimental measurements and then estimate the dosage for humans.
[450] The concentration of the active compound sodium salt in the pharmaceutical composition depends on the rate of absorption, inactivation and excretion of the active ingredient, the physicochemical properties of the active ingredient, the dosing schedule and dosage as well as other factors known to those skilled in the art. For example, the delivered amount is sufficient to treat the symptoms of hypertension. It is expected that the amount effective for treating endothelin-mediated disease will be higher than the amount of sulfonamide compound administered to treat bacterial infection.
[451] Typically a therapeutically effective dose should produce a serum concentration of the active ingredient of about 0.1 ng / ml to about 50 to 100 μg / ml. Pharmaceutical compositions typically should provide a dose of about 0.001 mg to about 2000 mg of compound per kg of body weight daily. Pharmaceutical dosage unit forms are prepared to provide from about 1 mg to about 1000 mg, preferably from about 10 to about 500 mg, of the active ingredient or composition of the essential ingredient per dosage unit form.
[452] The active ingredient may be administered at one time or in divided portions at timed intervals. The precise dosage and duration of treatment correlate with the disease to be treated and can, of course, be determined by using experimental tests known in the art or by estimation from in vitro or in vivo test data. It should be noted that concentrations and dosage values may also vary with the severity of the condition to be alleviated. In addition, for certain patients, a particular dosage regimen should be adjusted over time according to personal needs and the professional judgment of the person supervising or administering the composition, and the concentration ranges presented herein are by way of example only, and claimed. It is of course not intended to limit the scope or practice of the compositions described.
[453] Preferred derivatives include acid, salt, ester and prodrug forms. The derivative is chosen to be in a more stable form than the corresponding neutral compound. Amine salts such as, but not limited to, N, N'-dibenzylethylenediamine, chloroprocaine, choline, ammonia, diethanolamine and other hydroxyalkylamines, ethylenediamine , N-methylglucamine, procaine, N-benzylphenethylamine, 1-para-chlorobenzyl-2-pyrrolidin-1'-ylmethylbenzimidazole, diethylamine and other alkylamines, piperazine , Tris (hydroxymethyl) aminomethane, alkali metal salts such as, but not limited to, lithium, potassium and sodium, alkaline earth metal salts such as, but not limited to, barium, calcium and magnesium, Transition metal salts such as, but not limited to, iron, zinc, gold and silver, and other metal salts such as, but not limited to, aluminum, sodium hydrogen phosphate, disodium phosphate or bismuth salts. , Preferably sodium salts, more Preferably also includes, but is not limited to, salts of inorganic acids, such as, but not limited to, salts of inorganic acids, such as hydrochlorides and sulfates, salts of organic acids, such as, but not limited to, Pharmaceutical formulations comprising acetic acid, lactate, malate, tartrate, citrate, ascorbate, succinate, butyrate, valeric acid and fumarate or pharmaceutically acceptable esters or derivatives thereof of sulfonamide compounds Preferred salts are preferred. More preferred salts include sodium salts such as, but not limited to, sodium hydrogen phosphate salts and sodium salts, most preferably sodium salts.
[454] Thus, an effective concentration or amount of one or more compounds or pharmaceutically acceptable derivatives thereof provided herein is mixed with a pharmaceutical carrier or vehicle suitable for systemic, topical or topical administration to form a pharmaceutical composition. The compound is included in an amount effective to alleviate or treat an endothelin-mediated disease contemplated for treatment. The concentration of the active compound in the composition depends on the absorption, inactivation, rate of release, dosage schedule and dosage of the active compound, the particular formulation, as well as other factors known to those skilled in the art.
[455] The compositions are intended to be administered by any suitable route, including oral, parenteral, rectal and topical and topical, depending on the condition to be treated. For example, for the treatment of ophthalmic diseases such as glaucoma, intraocular and intravitreal injection formulations are contemplated. For oral administration, capsules and tablets are presently preferred. For parenteral administration, reconstitutions of lyophilized powders prepared as described herein are preferred. Compounds in liquid, semi-liquid or solid form are formulated in a manner suitable for the respective route of administration. Preferred dosage forms include parenteral and oral dosage forms.
[456] Solvents or suspending agents for parenteral, intradermal, subcutaneous or topical application may include the following ingredients: sterile diluents, for example, water for injection, saline solution, nonvolatile oils, polyethylene glycol, glycerin, propylene glycol or Synthetic solvents other than these; Antimicrobial agents such as benzyl alcohol and methyl parabens; Antioxidants such as ascorbic acid and sodium disulfide; Chelating agents such as ethylenediaminetetraacetic acid (EDTA); Buffers such as acetates, citrates and phosphates; And isotonicity modifiers such as sodium chloride or dextrose. Parenteral formulations may be encapsulated in ampoules, disposable syringes, and single or multiple dose vials made of glass, plastic or other suitable materials.
[457] When the compound exhibits insufficient solubility, a method of dissolving the compound can be used. Such methods are known to those skilled in the art and include, but are not limited to, the use of cosolvents such as dimethylsulfoxide (DMSO), the use of surfactants such as tween, or dissolution in aqueous sodium bicarbonate. It is not limited. Derivatives of the compounds, for example prodrugs of the compounds, can also be used to formulate effective pharmaceutical compositions.
[458] Upon mixing or addition with the sodium salt of the sulfonamide compound, the resulting mixture may be a solvent, suspending agent, emulsion, and the like. The form of the resulting mixture depends on a number of factors, including the intended dosage form and solubility of the compound in the selected carrier or vehicle. Effective concentrations are sufficient to alleviate the symptoms of the disease, disorder or condition being treated and can be measured experimentally.
[459] The dosage form may be in unit dosage form, eg, tablets, capsules, pills, powders, anhydrous powders for inhalers, granules, sterile parenteral or suspensions, and oral or suspensions, and suitable amounts of compounds, in particular thereof An oil-water emulsion containing a pharmaceutically acceptable salt, preferably sodium salt, is provided for administration to humans and animals. Pharmaceutically active compounds and derivatives thereof are typically formulated and administered in unit-dose or multi-dose form. Unit-dose form as used herein refers to physically discrete units suitable for human and animal patients and are packaged as known in the art. Each unit-dose contains a predetermined amount of a pharmaceutically active compound with the required pharmaceutical carrier, vehicle or diluent sufficient to produce the desired therapeutic effect. Examples of unit-dose forms include tablets or capsules individually packaged in ampoules and syringes. Unit-dose forms may be administered in portions or multiple times. Multiple-dose forms are multiple identical unit-dose forms packaged in a single container that can be administered in separate unit-dose forms. Examples of multi-dose forms include bottles or pints or gallon bottles of vials, tablets or capsules. Thus, a multiple dose form is a multiple unit-dose that does not separate upon packaging.
[460] The composition may contain together the following active ingredients: diluents such as lactose, sucrose, dicalcium phosphate or carboxymethylcellulose; Lubricants such as magnesium stearate, calcium stearate and talc; And binders such as starches, natural gums such as acacia gelatin, glucose, molasses, polyvinylpyrrolidine, cellulose and derivatives thereof, povidone, crospovidone and binders other than those known to those skilled in the art. Pharmaceutically administrable compositions of the liquid may, for example, dissolve, disperse or otherwise dissolve the active compound and pharmaceutical adjuvant as defined above in a carrier such as water, saline, aqueous dextrose, glycerol, glycol, ethanol, or the like. It can be prepared by mixing to form a solvent or suspending agent. In some cases, the pharmaceutical composition to be administered may contain a small amount of nontoxic auxiliary substances, such as wetting agents, emulsifying or solubilizing agents, pH buffers, and the like, for example, acetates, sodium citrate, cyclodextrin derivatives, sorbitan monolaurate, It may further contain triethanolamine sodium acetate, triethanolamine oleate and other reagents. Actual methods of preparing such dosage forms are known or will be apparent to those skilled in the art (eg, Remington's Pharmaceutical Sciences, Mack Publishing Company, Easton, Pa., 15th Edition, 1975). In any case, the composition or formulation to be administered will contain the active compound in an amount sufficient to alleviate the symptoms of the patient to be treated.
[461] Dosage forms or compositions containing active ingredients in the range of 0.005% to 100% can be prepared in balance with non-toxic carriers. For oral administration, pharmaceutically acceptable non-toxic compositions may contain any of the commonly used excipients such as pharmaceutical grade mannitol, lactose, starch, magnesium stearate, talc, cellulose derivatives, sodium croscarmellose, glucose, water It is formed by the incorporation of cross, magnesium carbonate, sodium saccharin and talc. Such compositions include, but are not limited to, solvents, suspensions, tablets, capsules, powders, anhydrous powders for inhalers, and sustained release formulations, such as, but not limited to, graft and microencapsulated delivery systems, and biodegradable, biocompatible polymers, such as Examples include collagen, ethylene vinyl acetate, polyanhydrides, polyglycolic acid, polyorthoesters, polylactic acid, and the like. Methods of preparing such formulations are known to those skilled in the art. Contemplated compositions may contain 0.01% to 100%, preferably 0.1 to 95%, typically 75 to 95% of the active ingredient.
[462] Salts of the active compounds, preferably sodium salts, can be prepared with carriers, such as sustained release formulations or coatings, which prevent the compound from rapidly exiting the body. The formulations may include other active compounds to obtain the desired combination of properties. Compounds of formula (1), or pharmaceutically acceptable salts and derivatives thereof, as described herein are advantageously known in the general field of value for the treatment of one or more of the diseases or medical conditions mentioned above for therapeutic or prophylactic purposes. Other pharmacological agents such as beta-adrenergic blockers (e.g. atenolol), calcium channel blockers (e.g. nifedipine), angiotensin converting enzyme (ACE) inhibitors (e.g. lisinopril), diuretics ( E.g. furosemide or hydrochlorothiazide), endothelin converting enzyme (ECE) inhibitors (e.g. phosphoramidone), neutral endopeptidase (NEP) inhibitors, HMGCoA reductase inhibitors, nitric oxide donors, antioxidants It may also be administered in conjunction with vasodilators, dopamine agonists, neuroprotectors, steroids, beta-agonists, anticoagulants or thrombolytics. Such combination therapies constitute, of course, additional aspects of the compositions and methods of treatment provided herein.
[463] 1.Formulations for Oral Administration
[464] Oral pharmaceutical dosage forms are solids, gels or liquids. Solid dosage forms are tablets, capsules, granules and bulk powders. Types of oral tablets include compressed, chewable lozenges and tablets, which can be enteric, sugar or thin skin. Capsules may be hard or soft gelatin capsules, and granules and powders may be provided in non-boiling or boiling form in combination with other ingredients known to those skilled in the art.
[465] In certain embodiments, the formulation is a solid dosage form, preferably a capsule or tablet. Tablets, pills, capsules, troches, and the like may contain the following ingredients, or compounds of similar properties: binders; diluent; Disintegrants; slush; Glidants; Sweeteners; And flavoring agents.
[466] Examples of binders include microcrystalline cellulose, gum tragacanth, glucose solution, acacia slime, gelatin solution, sucrose and starch paste. Lubricants include talc, starch, magnesium stearate or calcium stearate, calendula and stearic acid. Diluents include, for example, lactose, sucrose, starch, kaolin, salts, mannitol and dicalcium phosphate. Glidants include, but are not limited to, colloidal silicon dioxide. Disintegrants include croscarmellose sodium, sodium starch glycolate, alginic acid, corn starch, potato starch, bentonite, methylcellulose, agar and carboxymethylcellulose. Colorants may be, for example, tested water soluble FD and C dyes, mixtures thereof; And water insoluble FD and C dyes suspended in hydrated alumina. Sweeteners include sucrose, lactose, mannitol and artificial sweeteners such as sodium cyclate and saccharin, and many spray dried flavors. Flavoring agents include natural flavors extracted from plants, such as fruits, and synthetic mixtures of compounds that give rise to good taste, including but not limited to peppermint and methyl salicylate. Wetting agents include propylene glycol monostearate, sorbitan monooleate, diethylene glycol monolaurate and polyoxyethylene laural ether. Topicals include fatty acids, fats, lead, shellac, ammonia shellac and cellulose acetate phthalate. Thin films include hydroxyethylcellulose, sodium carboxymethylcellulose, polyethylene glycol 4000 and cellulose acetate phthalate.
[467] If oral administration is desired, the salt of the compound may be provided in a composition that protects it from the acidic environment of the stomach. For example, the composition may be formulated with enteric skin that preserves it in the stomach and releases the active compound in the intestine. The compositions may also be formulated in combination with antacids or other ingredients.
[468] If the dosage unit form is a capsule, it may contain, in addition to the substances of this type, a liquid carrier, for example a fatty oil. In addition, dosage unit forms may contain various other materials that modify the physical form of the dosage unit, such as a coating of sugar and other enteric formulations. The compounds may also be administered as components of elixirs, suspensions, syrups, wafers, sprinkles, chewing gums and the like. Syrups may contain, in addition to the active ingredient, sucrose as a sweetening agent and certain preservatives, dyes and colorings and flavoring agents.
[469] The active substance may also be mixed with other active substances which do not impair the desired action, or substances which complement the desired action, for example antacids, H2 blockers and diuretics. For example, when the compounds are used to treat asthma or hypertension, they can be used in combination with other bronchodilators and hypertensive agents, respectively. The active ingredient is a compound or salt thereof as described herein. It can contain up to about 98% by weight higher concentration of active ingredient.
[470] Pharmaceutically acceptable carriers included in tablets are binders, lubricants, diluents, disintegrants, colorants, flavors and wetting agents. Enteric bar tablets withstand the action of gastric acid due to enteric bar and dissolve or disintegrate in the neutral or alkaline intestine. Epithelial tablets are compressed tablets to which different layers of pharmaceutically acceptable substances are applied. Thin film tablets are compressed tablets coated with a polymer or other suitable coating. Multiple compressed tablets are compressed tablets made by one or more compression cycles using the pharmaceutically acceptable materials described above. Colorants can also be used in the dosage forms described above. Flavors and sweeteners can be used in compressed tablets, sugar-coated, multiple compressed chewable tablets. Flavoring and sweetening agents are particularly useful for shaping chewable tablets and lozenges.
[471] Liquid oral dosage forms include aqueous solutions, emulsions, suspensions, solvents reconstituted from non-boiling granules and / or effervescent preparations reconstituted from suspensions and effervescent granules. Aqueous liquids include, for example, elixirs and syrups. Emulsions are oil-in-water or water-in-oil.
[472] Elixir is a clear, sweet, hydroalcoholic formulation. Pharmaceutically acceptable carriers used in elixirs are solvents. The syrup is a concentrated aqueous solvent of sugars such as sucrose and may contain a preservative. Emulsions are two-phase systems in which one liquid is dispersed in the form of small pills throughout the other liquid. Pharmaceutically acceptable carriers used in emulsions are non-aqueous liquids, emulsifiers and preservatives. Suspensions use pharmaceutically acceptable suspending agents and preservatives. Pharmaceutically acceptable substances used in non-boiling granules to be reconstituted into liquid oral dosage forms include diluents, sweeteners and wetting agents. Pharmaceutically acceptable materials used in effervescent granules to be reconstituted into liquid oral dosage forms include sources of organic acids and carbon dioxide. Colorants and flavoring agents are used in all of the above dosage forms.
[473] Solvents include glycerin, sorbitol, ethyl alcohol and syrup. Examples of preservatives include glycerin, methyl and propylparabens, benzoic acid, sodium benzoate and alcohols. Examples of non-aqueous liquids used in emulsions include mineral oil and cottonseed oil. Examples of emulsifiers include gelatin, acacia, tragacanth, bentonite and surfactants such as polyoxyethylene sorbitan monooleate. Suspending agents include sodium carboxymethylcellulose, pectin, tragacanth, bum and acacia. Diluents include lactose and sucrose. Sweetening agents include sucrose, syrup, glycerin and artificial sweetening agents such as sodium cyclate and saccharin. Wetting agents include propylene glycol monostearate, sorbitan monooleate, diethylene glycol monolaurate and polyoxyethylene lauryl ether. Organic acids include citric acid and tartaric acid. Sources of carbon dioxide include sodium bicarbonate and sodium carbonate. Colorants include the tested water soluble FD and C dyes, and mixtures thereof. Flavoring agents include synthetic mixtures of compounds which give rise to a good taste and natural flavors extracted from plants, for example fruits.
[474] For solid dosage forms, solvents or solutions in, for example, propylene carbonate, vegetable oils or triglycerides are preferably encapsulated in gelatin capsules. Such solvents, and methods for their preparation and encapsulation, are described in US Pat. No. 4,328,245; 4,409,239 and 4,410,545. For liquid dosage forms, for example, liquid solutions in polyethylene glycol may be diluted with a sufficient amount of a pharmaceutically acceptable liquid carrier, such as water, for easy measurement for administration.
[475] In addition, liquid or semisolid oral formulations dissolve or disperse the active compounds or salts in vegetable oils, glycols, triglycerides, propylene glycol esters (such as propylene carbonate) and other carriers, and the solvents or solutions are hard or soft gelatin capsules. It can be prepared by encapsulating in a shell. Other useful formulations include those described in US Pat. Nos. 28,819 and 4,358,603.
[476] In one embodiment, the formulation is a solid dosage form, preferably a capsule or tablet. In a preferred embodiment, the formulation is at least one sulfonamide or sulfonamide salt, preferably at least one sodium hydrogen phosphate or sodium salts of the sulfonamide compound of formula 1, more preferably 10 to 100% by weight sodium salt, preferably 50 to 95% by weight, more preferably 75 to 85% by weight, most preferably 80 to 85% by weight; Diluent or binder, eg, lactose or microcrystalline cellulose, about 0-25%, preferably 8-15%; Disintegrants, for example modified starch or cellulose polymers, in particular crosslinked sodium carboxymethyl cellulose, for example croscarmellose sodium (crosscarmellose sodium NF is available from FMC Corporation of Philadelphia, Pennsylvania). Commercially available as DI-SOL) or sodium starch glycolate about 0-10%, preferably about 3-7%; And solid dosage forms, preferably capsules or tablets, containing lubricants such as magnesium stearate, talc and calcium stearate 0-2%. Disintegrants, such as croscarmellose sodium or sodium starch glycolate, provide for rapid destruction of the cellulosic matrix for immediate release of the active agent following degradation of the coating polymer. In all embodiments, the precise amounts of active and adjuvant components can be determined experimentally and correlate with the route of administration and the disease to be treated.
[477] In an exemplary embodiment, the formulation comprises about 80 to 90%, preferably about 83%, of one or more sodium salts of one or more sulfonamide compounds of Formula 1; Diluent or binder, eg, lactose or microcrystalline cellulose, about 10-15%, preferably about 11%; Disintegrants such as croscarmellose sodium or sodium starch glycolate about 1 to 10%, preferably about 5%; And lubricants such as capsules containing about 0.1 to 5%, preferably about 1%, magnesium stearate. Solid forms for administration as tablets are also contemplated herein.
[478] In an exemplary preferred embodiment, the formulation comprises 83% of one or more sodium salts of one or more sulfonamide compounds; Microcrystalline cellulose 11%; Disintegrants such as croscarmellose sodium or sodium starch glycolate 5%; And a capsule containing 1% magnesium stearate.
[479] The embodiments may also be formulated in the form of tablets which may optionally be coated. Tablets may contain the compositions described herein.
[480] In all embodiments, tablet and capsule formulations may be coated as known to those skilled in the art to modify or sustain degradation of the active ingredient. As such, for example, they can be coated with conventional enteric degradable coatings such as phenylsalicylate, lead and cellulose acetate phthalate.
[481] 2. Injections, Solvents and Emulsions
[482] Parenteral administration, generally characterized by injection subcutaneously, intramuscularly or intravenously, is also contemplated herein. Injectables can be prepared in conventional forms such as liquid solvents or suspensions, solid forms suitable for solvents or suspensions which are liquid before injection, or as emulsions. Suitable excipients are, for example, water, saline, dextrose, glycerol or ethanol. In addition, where necessary, the pharmaceutical compositions to be administered also contain small amounts of nontoxic auxiliary substances, such as wetting or emulsifying agents, pH buffers, stabilizers, solubility enhancers, and, for example, sodium acetate, sorbitan monolaurate, It may contain other agents such as triethanolamine oleate and cyclodextrin. Infusion of sustained or sustained release systems (eg, US Pat. No. 3,710,795) is also contemplated herein so that a constant level of dosage is maintained. The percentage of active compound contained in such parenteral compositions is highly dependent on its properties as well as the activity of the compound and the needs of the patient.
[483] Parenteral administration of the formulation includes intravenous, subcutaneous and intramuscular administration. Formulations for parenteral administration include sterile solvents that can be injected directly, subcutaneous injection tablets, sterile anhydrous soluble products that are easy to mix with the solvent just prior to use, such as the lyophilized powders described herein, Sterile suspending agents which can be directly injected, sterile anhydrous insoluble products and sterile emulsions which are easy to mix with the vehicle just prior to use. The solvent can be aqueous or non-aqueous.
[484] When administered intravenously, suitable carriers include physiological saline or phosphate buffered saline (PBS) and solvents containing thickening and solubilizing agents such as glucose, polyethylene glycol and polypropylene glycol and mixtures thereof.
[485] Pharmaceutically acceptable carriers used in parenteral preparations include, but are not limited to, aqueous vehicles, nonaqueous vehicles, antimicrobial agents, isotonic agents, buffers, antioxidants, local anesthetics, suspending and dispersing agents, emulsifying agents, concealing or chelating agents, and the like. Pharmaceutically acceptable substances.
[486] Examples of aqueous vehicles are Sodium Chloride Injection, Ringers Injection, Isotonic Dextrose Injection, Sterile Water Injection, Dextrose and Lactated Ringers ) Contains injections. Non-aqueous parenteral vehicles include vegetable nonvolatile oils, cottonseed oil, corn oil, sesame oil and peanut oil. Antimicrobial agents in bacteriostatic or fungal concentrations, many times, including phenol or cresol, mercury, benzyl alcohol, chlorobutanol, methyl and propyl p-hydroxybenzoic acid esters, thimerozal, benzalkonium chloride, and benzetonium chloride It can be added to parenteral preparations packaged in dosage containers. Isotonic agents include sodium chloride and dextrose. Buffers include phosphate and citrate. Antioxidants include sodium disulfate. Local anesthetics include procaine hydrochloride. Suspending and dispersing agents include sodium carboxymethylcellulose, hydroxypropyl methylcellulose and polyvinylpyrrolidone. Emulsifiers include Polysorbate 80 (Tween 80). Concealers or chelating agents of metal ions include EDTA. Pharmaceutical carriers also include ethyl alcohol, polyethylene glycol and propylene glycol for water miscible vehicles and sodium hydroxide, hydrochloric acid, citric acid or lactic acid for pH adjustment.
[487] The concentration of the pharmaceutically active compound is adjusted so that the injection provides an amount effective to produce the desired pharmacological effect. The exact dose depends on the age, weight and condition of the patient or animal as known in the art.
[488] Unit-dose parenteral preparations are packaged in ampoules, vials or needle syringes. All formulations for parenteral administration should be sterilized as is known and practiced in the art.
[489] By way of example, intravenous or intraarterial infusion of sterile aqueous solution containing the active compound is an effective mode of administration. Another embodiment is a sterile aqueous or oily solvent or suspending agent that contains the active substance to be injected as needed to produce the desired pharmacological effect.
[490] Injections are designed for local and systemic administration. Typically therapeutically effective dosages are formulated to contain an active compound at a concentration of about 0.1% w / w to at least about 90% w / w, preferably at least 1% w / w, to the tissue to be treated. The active ingredient may be administered at one time or may be administered at intervals divided into small doses. It is to be understood that the exact dosage and duration of treatment correlate with the tissue to be treated and can be determined by experimental use of known test protocols or by extrapolation from in vivo or in vitro test data. Note that concentrations and dosage values may also vary with the age of the person to be treated. In addition, for certain patients a particular dosage regimen should be adjusted over time according to personal needs and the professional judgment of the person instructing or directing the administration of the formulation and the concentration ranges described herein are exemplary only and claimed. It is of course not intended to limit the scope or practice of the formulation.
[491] The compounds may be micronized or suspended or derivatized in other suitable forms to produce more soluble active products or to produce prodrugs. The form of the resulting mixture depends on a number of factors, including the intended dosage form and solubility of the compound in the selected carrier or vehicle. Effective concentrations are sufficient to alleviate the symptoms of the condition and can be measured experimentally.
[492] According to the present invention, formulations containing certain sodium salts of sulfonamides, in particular those wherein R ⁸ is phenylacetyl, show an increase in stability compared to formulations containing neutral compounds. The data in Tables 5a and 5b are 4-chloro-3-methyl-5- (2- (2- (6-methylbenzo [d] [1,3] dioxol-5-yl) acetyl) compared to neutral compounds It reflects the increased solubility of the solution of sodium hydrogen phosphate and sodium salt of 3-thienylsulfonamido) isoxazole. These salts also show improved solubility over neutral compounds in aqueous media. As can be seen from Tables 5a and 5b, the sodium hydrogen phosphate salt is more stable than the neutral compound in the LABRASOL solution. Sodium salts have been found to be as stable as sodium hydrogen phosphate salts in certain aqueous formulations.
[493] saltmg / mlVehicleh ^a (%) ^bnone150LABRASOL2490.1 Sodium hydrogen phosphate100LABRASOL22.550.598.297.1 "5010% LABRASOL / water687.0 "25"689.4 "100DMSO2598.6 "100.01 M NaPO ₄ : PEG: EtOH (6: 3: 1) (pH 7.7)24.54898.6100 "2.4water17.596.5 "250.1% BSA in water9246.6 "25water694.5 "10Water: PEG 400: EtOH (6: 3: 1)6100 "100.01 M NaPO ₄ : PEG 400: EtOH (6: 3: 1) (pH 7.5)67.57 days 19 days10098.895.6 "5Deionized water244872938577 "5tap water243872918476 salt0.51Saline solution2496.9 ""5% dextrose2499.4 "0.570.75% PVP + 1.5% PG2474.4
[494] saltmg / mlVehicleh ^a (%) ^b"0.491.5% PVP + 3.0% PG2490.0 "1005% dextrose693.0 "10030% Sorbitol2493.2 "305% dextrose2492.2 "3020% Sorbitol2493.2 "205% dextrose2492.4 "2010% dextrose2493.4 "2010% Dextrose + 10% PG2495.6 "205% dextrose24 (13 degrees Celsius)93.7 "205% dextrose2490.1 "205% dextrose + K-phosphate buffer, 2.5% w / v pH 72092.6 "20"(pH 6.5)2489.4 """(pH 6)2484.6 """(pH 7.5)"93.4 ""5% dextrose + citrate buffer, 0.3% w / v pH 82192.9 ""10% dextrose + 10% PG + Na-phosphate buffer, 0.3% w / v pH 7.52490.7 """24 (4 degrees Celsius)97.4 """(pH 8)"(4 ℃)96.4 ""10% dextrose + 10% PG + citrate buffer, 0.3% w / v pH 7.424 (4 degrees Celsius)97.6 ""10% Dextrose + 10% PG24 (4 degrees Celsius)97.6 "3010% dextrose + 10% PG + citrate buffer, 0.3% w / v pH 7.524 (4 degrees Celsius)98.0 "205% dextrose + 5% PG + citrate buffer, 0.3% w / v pH 7.526 (4 degrees Celsius)97.2 "10010% dextrose + 10% PG + citrate buffer, 0.3% w / v pH 7.52494.2 "205% dextrose + citrate buffer, 0.3% w / v pH 7.527 (4 ℃)96.6 "10030% Sorbitol2493.2 "305% dextrose2492.2 "3020% Sorbitol2493.2 "205% dextrose2492.4 "2010% dextrose2493.4 "2010% Dextrose + 10% PG2495.6 "205% dextrose2490.2 "205% dextrose25 (4 ℃)93.7 "205% dextrose + 5% buffer (pH 7.0)2492.6^a time according to the preparation of the formulation ^b 4-chloro-3-methyl-5- (2- (2- (6-methylbenzo [d] [1,3] di) as measured by high performance liquid chromatography analysis Residual rate of oxol-5-yl) acetyl) -3-thienylsulfonamido) -isoxazole
[495] In many instances, solutions of sodium salts, including sodium salts and sodium hydrogen phosphate salts, show improved stability compared to neutral compounds. These salts also show improved solubility over neutral compounds in aqueous media.
[496] 3. Lyophilized powder
[497] Of particular interest are lyophilized powders that can be reconstituted for administration as solvents, emulsions and other mixtures. They can also be formulated as solids or gels.
[498] In certain embodiments, formulations of sodium hydrogen phosphate or sodium, preferably sodium salts, of sulfonamide compounds with increased stability compared to formulations of neutral sulfonamides are provided. Specifically, formulations of the sulfonamide sodium salt as sterile, lyophilized powders are provided. These powders have been found to have increased stability compared to formulations of neutral sulfonamides.
[499] Sterile, lyophilized powders are prepared by dissolving the sodium salt in sodium phosphate buffered solution containing dextrose or other suitable excipients. Subsequent sterile filtration of the solution followed by lyophilization under standard conditions known to those skilled in the art provides the desired formulation. In brief, lyophilized powders are approximately neutral to about 1 to 20%, preferably about 5 to 15% of dextrose, sorbitol, fructose, corn syrup, xylitol, glycerin, glucose, sucrose or other suitable formulations. It is prepared by dissolving in a suitable buffer of pH, for example citrate, sodium phosphate or potassium phosphate or buffers other than those known to those skilled in the art. Next, a selected salt of sulfonamide, preferably sodium salt (about 1 g of salt / 10-100 g of buffer solution, typically about 1 g / 30 g), is preferably at a temperature above room temperature, more preferably about 30 to 35 ° C. To the resulting mixture is added and stirred until dissolved. The resulting mixture is diluted by adding additional buffer (the resulting concentration of salt is reduced by about 10-50%, typically about 15-25%). The resulting mixture is sterile filtered or treated to remove particulates, ensure sterility and distribute into lyophilized vials. Each vial will contain a single dose (100-500 mg, preferably 250 mg) or multiple doses of sulfonamide salts. Lyophilized powders can be stored under suitable conditions, for example from about 4 ° C. to room temperature. Detailed descriptions of exemplary processes are set forth in the Examples.
[500] Reconstitution of the lyophilized powder into water for injection provides a formulation for use in parenteral administration of the sodium salt of sulfonamide. For reconstitution, about 1 to 50 mg, preferably 5 to 35 mg, more preferably about 9 to 30 mg is added per ml of sterile water or other suitable carrier. The exact amount depends on the indication to be treated and the compound selected.
[501] In certain embodiments, the formulation contains a lyophilized solid containing at least one sodium hydrogen phosphate or sodium, preferably sodium salt, of at least one sulfonamide compound of Formula 1, and also contains at least one of the following components:
[502] Buffers such as sodium or potassium phosphate, or sodium citrate or potassium citrate;
[503] Solubilizers such as LABRASOL, DMSO, bis (trimethylsilyl) acetamide, ethanol, propylene glycol (PG) or polyvinylpyrrolidine (PVP); And
[504] Sugars or carbohydrates such as sorbitol or dextrose.
[505] In a more preferred embodiment, the formulation comprises at least one sodium hydrogen phosphate or sodium, preferably sodium salt of at least one sulfonamide of formula (1); Buffers such as sodium or potassium phosphate, or sodium citrate or potassium citrate; And sugars or carbohydrates such as sorbitol or dextrose.
[506] In the most preferred embodiment, the formulation comprises at least one sodium salt of sulfonamide compound; Sodium phosphate buffer; And dextrose. Methods of making these formulations are illustrated in the Examples.
[507] 4. Topical Administration
[508] Topical mixtures are prepared as described for local and systemic administration. The resulting mixture may be a solution, suspension, emulsion, etc. and may be creams, gels, ointments, emulsions, solvents, elixirs, lotions, suspensions, tinctures, pastes, foams, aerosols, irrigation, sprays, suppositories, bandages, skin patches or It may be in a formulation other than suitable for topical administration.
[509] Sodium salts and other derivatives of the compounds can be formulated as aerosols, for example, for topical application by inhalation. For example, describe aerosols for steroid delivery useful for treating inflammatory diseases, especially asthma. US Patent Nos. 4,044,126, 4,414,209 and 4,364,923. These formulations for administration to the respiratory tract may be in the form of aerosols or solvents for nebulizers, alone or in admixture with inert carriers, for example lactose, or for dispersing powders for blowing. In this case, the diameter of the particles of the formulation will typically be less than 50 microns, preferably less than 10 microns.
[510] The sodium salts of the compounds can be used in the form of gels, creams, and lotions for topical or topical application, for example topical application to the skin and mucous membranes, such as the eye, and for application to the eye or intranasal or spinal cord. It can be formulated. Topical administration is contemplated for transdermal delivery and also for administration to the eye or mucosa or inhalation. Costly solvents may also be administered in which the active ingredient alone or in admixture with other pharmaceutically acceptable excipients.
[511] These solvents, especially those intended for ophthalmic use, may be formulated as 0.01% to 10% isotonic solvents of pH about 5-7 with suitable salts.
[512] 5. Products
[513] Derivatives of the compounds, in particular salts, acids, esters and preferably sodium salts, antagonize the effects of endothelin in packaging materials, packaging materials, alleviate symptoms of endothelin-mediated diseases, or endothelin to ET receptors. Sodium salts of the compounds provided herein, and compounds or salts thereof, that are effective for inhibiting binding to an IC ₅₀ of less than about 10 μM, antagonize the effects of endothelin, treat endothelin-mediated diseases, or treat endothelin peptides to the ET receptor. It can be packaged as a product containing a label indicating that it is used to inhibit binding.
[514] 6. Formulations for Other Routes of Administration
[515] Depending on the condition to be treated, other routes of administration, such as topical application, transdermal patches, rectal administration, are also contemplated herein.
[516] For example, pharmaceutical dosage forms for rectal administration are rectal suppositories, capsules and tablets for systemic effects. Rectal suppository as used herein refers to a solid for rectal insertion that melts or softens at body temperature to release one or more pharmacological or therapeutically active ingredients. Pharmaceutically acceptable substances used in rectal suppositories are agents that raise the base or vehicle and melting point. Examples of bases include cocoa butter (theobroma oil), glycerin-gelatin, carbowax, (polyoxyethylene glycol) and suitable mixtures of mono-, di- and triglycerides of fatty acids. Combinations of the various bases can be used. Formulations that raise the melting point of suppositories include spermaceti and beeswax. Rectal suppositories can be prepared by the compression method or the molding method. Typical weights of rectal suppositories are about 2-3 gm.
[517] Tablets and capsules for rectal administration are prepared by the same method using the same pharmaceutically acceptable materials as for oral dosage forms.
[518] E. Evaluation of Bioactivity of Compounds
[519] Standard physiological, pharmacological and biochemical methods can be used to test the compounds to confirm that they have the biological activity of the endothelin peptide or the ability to interfere or inhibit the endothelin peptide. Compounds that exhibit in vitro activity, for example, the ability to bind or compete with one or more endothelin peptides for binding to the endothelin receptor, may be used to distinguish the method of isolation of the endothelin receptor and the specificity of the endothelin receptor. It may be used in a method and is a subject for use in a method of treating an endothelin-mediated disease.
[520] As such, other preferred compounds of Formulas 1 and 2, other than those specifically identified herein, which are endothelin antagonists or agonists, can be identified using screening assays.
[521] 1. Identification of compounds that modulate the activity of endothelin peptides
[522] Compounds are tested for their ability to modulate the activity of endothelin-1. Many assays for assessing the ability of compounds to modulate the activity of endothelin are known to those of skill in the art. See US Pat. No. 5,114,918, Ishikawa et al. ; EP A1 0 436 189, BANYU PHARMACEUTICAL CO., LTD. (7 October 1991); Borges et al. (1989) Eur. J. Pharm. 165 : 223-230; Filep et al. (1991) Biochem. Biophys. Res. Commun. 177 : 171-176]. In vitro studies include in vivo studies [see, eg, US Pat. No. 5,114,918, Ishikawa et al .; EP A1 0 436 189, BANYU PHARMACEUTICAL CO., LTD. (October 7, 1991) and the pharmaceutical activity evaluated thereby. This test is a combination of the ET _A and ET _B receptors present on the described in the examples herein, and separated from the manipulation by genetic engineering cell lines to express ET _A and ET _B receptors on their cell surface membrane Include the ability to compete for.
[523] The properties of potential antagonists can be expressed as a function of their ability to inhibit endothelin-induced activity in vitro using the rat's uterus, trachea and vasculature, as well as the hepatic vein and aorta of a particular tissue, e. [References: For example, Borges, R., Von Grafenstein, H. and Knight, DE, “Tissue selectivity of endothelin,” Eur. J. Pharmacol. 165 : 223-230 (1989). The ability to act as endothelin antagonists in vivo can be tested in hypertensive rats, ddy mice or other validated animal models. See Kaltenbronn et al. (1990) J. Med. Chem. 33 : 838-845; U.S. Patent 5,114,918, Ishikawa et al. (1990); And EP A1 0 436 189, BANYU PHARMACEUTICAL CO., LTD. (October 7, 1991); Bolger et al. (1983) J. Pharmacol. Exp. Ther. 225 : 291-309. The results of the animal model can be used to assess pharmaceutical efficacy and determine pharmaceutically effective doses. Potential agonists can also be assessed using in vitro and in vivo assays known to those skilled in the art.
[524] Endothelin activity can be confirmed by the ability of test compounds to stimulate contraction of the thoracic aorta of isolated rats. See Borges et al. , "Tissue selectivity of endothelin," Eur. J. Pharmacol. 165 : 223-230. To perform the assay, the endothelium is stripped off and the yun fragment is placed under tension in the tissue bath and treated with endothelin in the presence of the test compound. Record changes in endothelin-induced tension. Dose response curves can be used to provide information about the relative inhibitory capacity of the test compound. Tissues other than heart, skeletal muscle, kidney, uterus, trachea, and vas deferens can be used to assess the effect of certain test compounds on tissue contraction.
[525] Endothelin isotype specific antagonists are characterized by the ability of test compounds to interfere with the binding of different endothelin-receptor subtypes of endothelin to different tissues or cells or the biological action of endothelin or endothelin isotypes. [Reference: Takayanagi et al. (1991) Reg. Pep. 32 : 23-37, Panek et al. (1992) Biochem. Biophys. Res. Commun. 183 : 566-571. For example, the ET _B receptor can be expressed in vascular endothelial cells, possibly controlling the release of prostacyclin and endothelial-induced relaxation factors . De Nucci et al. (1988) Proc. Natl. Acad. Sci. USA 85 : 9797]. The ET _A receptor is not detected in cultured endothelial cells expressing the ET _B receptor.
[526] Inhibition of the binding of the compound or binding of the endothelin to the ET _B receptor was observed in the endothelin-1 of prostacyclin as measured by its prominent stable metabolite, 6-keto PGF _{1α ′} , from cultured bovine aortic endothelial cells -Can be evaluated by measuring the inhibition of mediated release [Ref . Filep et al. (1991) Biochem. and Biophys Res. Commun. 177 : 171-176]. As such, the relative affinity of the compounds for different endothelin receptors can be assessed by measuring dose response curves that inhibit tissues with different receptor subtypes.
[527] Using this assay, relative substitutions of compounds to the ET _A and ET _B receptors have been assessed and can be evaluated. Those having the desired properties such as specific inhibition of the binding of endothelin-1 are selected. Selected compounds that exhibit desirable activity can be therapeutically useful and can be tested for such use using the assays described above to assess in vivo efficacy therefrom. See, US Pat. No. 5,248,807; US Patent No. 5,240,910; US Patent No. 5,198,548; US Patent No. 5,187,195; US Patent No. 5,082,838; US Patent No. 5,230,999; Published Canadian patent applications 2,067,288 and 20719393; Published British Patent Application No. 2,259,450; Published international PCT application WO 93/08799; Benigi et al. (1993) Kidney International 44 : 440-444; And Nirei et al. (1993) Life Sciences 52 : 1869-1874. Compounds exhibiting in vitro activity related to in vivo efficacy can be formulated into suitable pharmaceutical compositions and then used as therapeutic agents.
[528] The compounds may also be used in methods to identify and isolate endothelin-specific receptors and to aid in the design of compounds that are more effective endothelin antagonists or agonists or more specific for particular endothelin receptors.
[529] 2. Isolation of Endothelin Receptors
[530] Provided are methods for identifying endothelin receptors. In carrying out the method, one or more compounds are bound to a support and used in affinity purification of the receptor. By selecting compounds with specific specificities, separate subclasses of the ET receptor can be identified.
[531] Methods known to those skilled in the art to bind one or more compounds to endothelin by covalent or other linkages to suitable resins, such as Affi-gels. See Schvartz et al. (1990) Endocrinology 126 : 3218-3222. The bound compound may be specific for the ET _A or ET _B receptor or other subclass of receptors.
[532] The resin is generally pre-equilibrated with a suitable buffer at physiological pH (7-8). The composition containing the solubilized receptor from the selected tissue is mixed with the resin to which the compound is bound and the receptor is eluted selectively. Receptors can be identified by methods other than testing them for binding to endothelin isopeptides or analogs or identifying and characterizing proteins. Methods of preparing receptors, resins and eluates can be carried out by modification of standard protocols known to those skilled in the art . See Schvartz et al. (1990) Endocrinology 126 : 3218-3222.
[533] Methods other than classifying receptor types based on different affinities for the compounds herein are provided. Assays described herein that determine the affinity for endothelin receptors of selected compounds can also be used to classify receptor subtypes based on affinity for particular compounds provided herein. In particular, an unknown receptor can be identified as an ET _A or ET _B receptor by measuring the binding affinity of the unknown receptor for a compound provided herein having a known affinity which is superior to another receptor for one receptor. Such preferential interactions are useful for determining specific diseases that can be treated with a compound prepared as described herein. For example, compounds that have a high affinity for the ET _A receptor and have little affinity for the ET _B receptor are subjects for use as hypertensives; Compounds that preferentially interact with the ET _B receptor are subjects for use as anti-asthmatic agents.
[534] The following examples are merely illustrative and are not intended to limit the scope of the invention.
[535] Example 1
[536] N- (4-bromo-3-methyl-5-isoxazolyl) -2- (aminocarbonyl) thiophen-3-sulfonamide
[537] Carbonyldiimidazole (485 mg, 2.99 mmol) was added N- (4-bromo-3-methyl-5-isoxazolyl) -2-carboxythiophen-3-sulfonamide (1 g, 2.72 mmol) in THF (10 ml). To a solution at room temperature. The mixture is stirred for 15 minutes. Then aqueous NH ₃ (5 ml) is added and the mixture is stirred at room temperature for 30 minutes. The solvent is evaporated and the residue is partitioned between EtOAc and 1N HCl. The organic layer is dried (MgSO ₄ ). The solid is filtered off and the filtrate is concentrated. The oily residue was recrystallized from EtOAc to give N- (4-bromo-3-methyl-5-isoxazolyl) -2- (aminocarbonyl) thiophen-3-sulfonamide (946 mg, 95% yield) as a white solid.
[538] Example 2
[539] N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(3,4-methylenedioxy) benzoyl] thiophene-3-sulfonamide
[540] A. N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[(N-methoxy-N-methyl) aminocarbonyl] thiophene-3-sulfonamide
[541] N- (4-bromo-3-methyl-5-isoxazolyl) -2- in the same manner as described in Example 1 except that N, O-dimethylhydroxylamine is used instead of ammonium hydroxide [(N-methoxy-N-methyl) carboxamide] thiophene-3-sulfonamide is prepared. The yield is 90%.
[542] B. N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[(3,4-methylenedioxy) benzoyl] thiophene-3-sulfonamide
[543] Freshly prepared (3,4-methylenedioxy) phenyl magnesium bromide (1.28 g of (3,4-methylenedioxy) bromobenzene and 172 mg of Mg flakes) was added to N- (4-bromo-3 in THF (10 ml). To a solution of -methyl-5-isoxazolyl) -2-[(N-methoxy-N-methyl) aminocarbonyl] thiophen-3-sulfonamide (A) (652 mg, 1.59 mmol) is added at room temperature . The resulting mixture is refluxed for 30 minutes. For workup, the mixture is cooled to room temperature and quenched with 1N HCl (10 ml). Next, THF is evaporated. The aqueous residue is partitioned between 1N HCl and EtOAc. The organic layer was concentrated and the residue was purified by HPLC to give N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(3,4-methylenedioxy) benzoyl having a melting point of 47 to 49 ° C. ] Thiophen-3-sulfonamide (90 mg, 12% yield) is obtained as a dark yellow powder.
[544] Example 3
[545] N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(2-hydroxyphenyl) aminocarbonyl] thiophen-3-sulfonamide
[546] N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(2- in the same manner as described in Example 1, except that 3-aminophenol is used instead of ammonium hydroxide. Hydroxyphenyl) aminocarbonyl] thiophene-3-sulfonamide. The product was purified by HPLC to give N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(2-hydroxyphenyl) aminocarbonyl] thiophen-3- at a melting point of 42 to 44 ° C. Sulfonamide (50 mg, 18% yield) is obtained as a pale yellow solid.
[547] Example 4
[548] N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(3,4-methylenedioxy) phenylacetyl] thiophene-3-sulfonamide
[549] Same method as described in Example 2, except using piperonyl magnesium chloride in place of (3,4-methylenedioxy) phenyl magnesium bromide and stirring the reaction mixture overnight at room temperature instead of refluxing for 30 minutes N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(3,4-methylenedioxy) phenylacetyl] thiophene-3-sulfonamide was prepared. The crude mixture was purified by HPLC to give N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(3,4-methylenedioxy) phenylacetyl] thiophen-3-sulfonamide (20 mg , 40% yield) as a yellow oil.
[550] Example 5
[551] N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(3,4-methylenedioxy) phenylacetyl] thiophene-3-sulfonamide
[552] N- (4-chloro-3-methyl-5-isoxazolyl) -2 instead of N- (4-bromo-3-methyl-5-isoxazolyl) -2-carboxythiophen-3-sulfonamide N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(3, in the same manner as described in Example 4 except for using carboxythiophen-3-sulfonamide 4-Methylenedioxy) phenylacetyl] thiophene-3-sulfonamide is prepared. HPLC purification of N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(3,4-methylenedioxy) phenylacetyl] thiophene-3-sulfonamide (3 g, 50% yield) Obtained as a yellow solid with a melting point of 35 to 38 ° C.
[553] Example 6
[554] 4-chloro-3-methyl-5- (2- (2- (6-methylbenzo [d] [1,3] dioxol-5-yl) acetyl) -3-thienylsulfonamido) isoxazole and N-, also called N- (4-chloro-3-methyl-5-isoxazolyl) -2- [2-methyl-4,5- (methylenedioxy) phenylacetyl] thiophene-3-sulfonamide (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methyl] phenylacetyl-3-thiophenesulfonamide
[555] A. (3,4-methylenedioxy) -6-methylbenzyl chloride
[556] To a 1: 1 mixture of ethyl ether (100 ml) and concentrated HCl (100 ml) is added (3,4-methylenedioxy) toluene (10 ml) at 0 ° C. Formaldehyde (20 ml, 37% in water) is then added dropwise. The reaction is stirred at 0 ° C. for 2 hours and at room temperature for an additional 10 hours. The reaction mixture is diluted with ethyl ether (100 ml) and the two layers are separated. The organic layer is dried (MgSO ₄ ), the solid is filtered off and the filtrate is concentrated. The residue is then heated with hexane (200 ml) and the insolubles are filtered off with a hot solution. The filtrate was concentrated to give (3,4-methylenedioxy) -6-methylbenzyl chloride (9.4 g, 63% yield) and bis [(3,4-methylenedioxy) -6-methyl] phenylmethane (3.6 g) Is obtained as a white solid. This mixture is used in the next step without further purification.
[557] B. N- (4-Chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methyl] phenylacetyl-3-thiophenesulfonamide
[558] N- (4- in the same manner as described in Example 5 except for using (3,4-methylenedioxy) -6-methylbenzyl chloride instead of (3,4-methylenedioxy) benzyl chloride Chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methyl] phenylacetyl-3-thiophenesulfonamide is synthesized. The crude product was purified by preparative HPLC to give N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methyl] phenylacetyl-3-thiophene Sulfonamide is obtained as a yellow powder (71% yield, melting point 42-45 ° C.).
[559] Example 7
[560] 4-chloro-3-methyl-5- (2- (2- (6-methylbenzo [d] [1,3] dioxol-5-yl) acetyl) -3-thienylsulfonamido) isoxazole, Sodium salt
[561] A. (4-Chloro-3-methyl-5- (2- (2- (6-methylbenzo [d] [1,3] dioxol-5-yl) acetyl) -3-thienylsulfonamido) Preparation of Isoxazole
[562] 1. Preparation of 5-chloromethyl-6-methylbenzo [d] [1,3] dioxol
[563] To a mixture of methylene chloride (130 L), concentrated HCl (130 L) and tetrabutylammonium bromide (1.61 Kg) was added 5-methylbenzo [d] [1,3] dioxole (10 Kg), followed by formaldehyde (14 L, 37% by weight in water) is added slowly. The mixture is stirred overnight. The organic layer is separated, dried over magnesium sulfate and concentrated to an oil. Hexane (180 L) is added and heated until boiling. The hot hexane solution is decanted from the concentrated oily residue and evaporated to give almost pure 5-chloromethyl-6-methylbenzo [d] [1,3] dioxol as a white solid. Recrystallization from hexane (50 L) gives 5-chloromethyl-6-methylbenzo [d] [1,3] dioxol (80% recovery after recrystallization).
[564] 2. 4-Chloro-3-methyl-5- (2- (2- (6-methylbenzo [d] [1,3] dioxol-5-yl) acetyl) -3-thienylsulfonamido) Formation of solazole
[565] A portion of a solution of 5-chloromethyl-6-methylbenzo [d] [1,3] dioxol (16.8 g, 0.09 mol) in tetrahydrofuran (THF) (120 mL) was added to magnesium powder (3.3 g) in THF (120 mL). To a well stirred slurry of 0.136 g-atoms, alpha or Johnson-Matthey, -20 + 100 mesh) is added at room temperature. The resulting reaction mixture is warmed to about 40-45 ° C. for about 2-3 minutes to initiate the reaction. Once magnesium is activated by heating, the reaction begins and the mixture is cooled and maintained at a temperature below about 8 ° C. Magnesium can be activated with dibromoethane instead of heat.
[566] The flask containing the reaction mixture is cooled and the remaining solution of 5-chloromethylbenzo [d] [1,3] dioxol is added dropwise for 1.5 hours while maintaining the internal temperature below 8 ° C. Temperature control is important: Wurtz coupling occurs when Grignard is produced and kept below 8 ° C. Long periods of time at higher temperatures facilitate the Buttz coupling path. Butz coupling can be avoided by using high quality Mg, keeping the Grignard temperature below about 8 ° C. and vigorously stirring. The reaction proceeds well at −20 ° C. and any temperature below 8 ° C. at which Grignard is formed is acceptable. The color of the reaction mixture turns green.
[567] The reaction mixture is stirred for an additional 5 minutes at 0 ° C. and N ² -methoxy-N ² -methyl-3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) in anhydrous THF (90 mL) 2-thiophenecarboxamide (6.6 g, 0.018 mol) is charged to an additional funnel. The reaction mixture was degassed twice, and then a solution of N ² -methoxy-N ² -methyl-3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophencarboxamide was removed. Add at 0 ° C. for 5 minutes. TLC (silica, 12% MeOH / CH ₂ Cl ₂ ) of the reaction mixture immediately after addition is N ² -methoxy-N ² -methyl-3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) It shows that 2-thiophenecarboxamide is not present.
[568] The reaction mixture is transferred to a flask containing 1N HCl (400 mL, 0.4 mol HCl, stirred in an ice bath), the mixture is stirred for 2-4 minutes, then transferred to a separatory funnel and diluted with ethyl acetate (300 mL). . After shaking the layers are separated. The aqueous layer is extracted with additional ethyl acetate (150 mL) and the combined organics are washed with half brine. After separation, THF is removed by drying the organic layer over sodium sulfate and concentrated at about 39 ° C. under reduced pressure.
[569] B. 4-Chloro-3-methyl-5- (2- (2- (6-methylbenzo [d] [1,3] dioxol-5-yl) acetyl) -3-thienylsulfonamido) Preparation of Soazole, Sodium Salt
[570] The product from A is redissolved in ethyl acetate and washed with saturated NaHCO ₃ (5 × 50 mL) until the wash is colorless. The solution is washed with brine, dried over Na ₂ SO ₄ and concentrated in vacuo to give a semicrystalline yellow residue. 100 mL of CH ₂ Cl ₂ is added to the solution and the mixture is stirred under nitrogen for 5 to 10 minutes until a microcrystalline product is formed. Ether (150 mL) is added and the mixture is stirred for a suitable time (eg 10 minutes). The product is separated by filtration, washed with a CH ₂ Cl ₂ / ether (1: 2) (30 mL) mixture, and then ether (30 mL) and dried under reduced pressure. When prepared according to the specific embodiments set forth above, the title product is obtained in an amount of 7.3 g and of about 85% purity (HPLC, RP, 40% acetonitrile / water, 0.1% TFA neutralized to pH 2.5 using ammonia) Ratic conditions, 1 mL / min).
[571] The salt product is dissolved in water (600 mL) at 10 ° C., the solution is stirred for a short time (eg 3 minutes) and then filtered through a layer of filter paper (eg 3 filter papers) with an inhaler . In some cases, large amounts of impurities (10% or more) not soluble in water slow the filtration process extremely. This problem can be avoided by using larger sized filter paper during filtration. Usually, when the purity of the salt is 90% or more, there is no problem in filtration.
[572] The green slightly turbid solution obtained from filtration is cooled in an ice bath and acidified to pH 2 with acid, for example 4N HCl. When the pH of the solution reaches 2, the product precipitates out as a milky white non-filterable material. Excess 4N HCl is slowly added dropwise to allow the product to form a fine, easily filterable precipitate. The pale yellow precipitate is filtered off, washed with water until neutral and pressurized on the filter paper to remove excess water. The free acid obtained typically has a purity of 95% as determined by HPLC.
[573] The product in free acid form is dissolved in ethyl acetate (about 100 mL) and washed with brine (30 mL) to remove water. The dehydrated solution was shaken with cold saturated NaHCO ₃ solution (2 × 30 mL) and then shaken again with brine, dried over Na ₂ SO ₄ and concentrated under vacuum (crude temperature below 40 ° C.) A light yellow foam is obtained. After complete removal of ethyl acetate from this product, CH ₂ Cl ₂ (100 mL) is added and the mixture is stirred for 5 to 10 minutes until the product crystallizes. Ether (150 mL) is added and stirring is continued for at least 10 minutes. The resulting solid is separated by filtration, washed with a mixture of CH ₂ Cl ₂ / ether (1: 2) (30 mL), and then with ether (30 mL) and dried under reduced pressure. When purified in this manner, 4-chloro-3-methyl-5- (2- (2- (6-methylbenzo [d] [1,3] dioxol-5-yl) acetyl) -3-thienyl Sulfonamido) isoxazoles, sodium salts are obtained in high yield (5.7 g, 68%) and in high purity (98.2% purity by HPLC). If the initial purity is high enough, following the procedure, the product can be further purified by recrystallization from EtOH / methyl t-butylether (MTBE).
[574] C. 4-Chloro-3-methyl-5- (2- (2- (6-methylbenzo [d] [1,3] dioxol-5-yl) acetyl) -3-thienylsulfonamido) N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methyl] phenylacetyl-3-also referred to as azole, sodium hydrogen phosphate salt Thiophenesulfonamide, sodium hydrogen phosphate
[575] N- (4-Chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methyl] phenylacetyl-3-thiophenesulfonamide (1.1492 g, 2.5263 mmol ) And deionized water (25 mL) and acetonitrile (25 mL) are added to a solid mixture of dibasic sodium phosphate (0.3468 g, 2.5263 mmol). The resulting mixture is shaken well and warmed to 50 ° C. to give a clear solution which is filtered. The filtrate was frozen at -78 ° C and lyophilized to yield the salt as a yellow powder ( 1.50 g).
[576] Example 8
[577] Formulation of Sulfonamide Sodium Salt as Lyophilized Powder
[578] 4-Chloro-3-methyl-5- (2- (2- (6-methylbenzo [d] [1,3] dioxol-5-yl) acetyl) -3-thienylsulfonamido for parenteral administration Formulation of isoxazoles and sodium salts
[579] Sterile water for injection, 3200 mL of USP, was added to a 4 L graduated cylinder to prepare phosphate buffer. Dibasic sodium phosphate heptahydrate, USP (21.44 g) is added to sterile water and the mixture is stirred for 5 minutes or until the solid is dissolved. Monobasic sodium phosphate, USP (11.04 g) is added and the mixture is stirred until the solid is dissolved. Dilute the solution to 4.0 L and stir. 3000 g of sodium phosphate buffer is added to an 8 L beaker. Dextrose, USP (200.0 g) is added and the mixture is warmed to 30-35 ° C. in a water bath and stirred until a complete solution is formed. 4-chloro-3-methyl-5- (2- (2- (6-methylbenzo [d] [1,3] dioxol-5-yl) acetyl) -3-thienylsulfonamido) isoxazole, Sodium salt (100.0 g) is added with good mixing. This mixture is stirred for at least 10 minutes or until a solution is formed.
[580] After the sodium salt is dissolved, the solution is removed from the water bath, diluted to 4000 g with sodium phosphate buffer and stirred for 5 minutes. This solution is sterile filtered using a sterile 0.22 micron pre-size Durapore Millipak 200 filter. The filtered solution is filled into sterile vials and lyophilized under standard conditions. Stopper the vial. The lyophilized product is then reconstituted with 9.4 mL ether or 19.4 mL water for injection to obtain a final concentration of 25 mg / mL or 12.5 mg / mL, respectively.
[581] Example 9
[582] N- (4-bromo-3-methyl-5-isoxazolyl) thiophene-2-sulfonamide
[583] 5-amino-4-bromo-3-methylisoxazole (177 mg, 1.0 mmol) in anhydrous tetrahydrofuran (THF, 2 mL) was suspended in sodium hydride in anhydrous THF (1 mL) (60% dispersion in mineral oil, 90 mg) , 2.2 mmol) is added at 0 to 5 ° C. Stir at 0-5 [deg.] C. for 5 minutes, then stir the reaction at RT for 10 minutes to complete the reaction. The reaction mixture is recooled to 0 ° C. and thiophene-2-sulfonyl chloride (200 mg, 1.1 mmol) dissolved in anhydrous THF (2 mL) is added dropwise. Stirring is continued for 1 hour; During this period the reaction mixture slowly reaches ambient temperature. Remove THF under reduced pressure. The residue is dissolved in water (10 ml), the pH is adjusted to 10-11 by addition of 5N sodium hydroxide solution and extracted with ethyl acetate (3 × 10 ml) to remove neutral impurities. The aqueous layer is acidified with concentrated HCl (pH 2-3) and extracted with methylene chloride (3 x 10 ml). The combined organic layers are dried over anhydrous magnesium sulfate and concentrated under reduced pressure to afford N- (4-bromo-3-methyl-5-isoxazolyl) thiophene-2-sulfonamide. Recrystallization with hexane / ethyl acetate yields a pure material (110 mg, 34% yield) with a melting point of 125-127 ° C.
[584] Example 10
[585] N- (4-bromo-3-methyl-5-isoxazolyl) -5- (3-isoxazolyl) thiophene-2-sulfonamide
[586] A solution of 5-amino-4-bromo-3-methylisoxazole (177 mg, 1.0 mmol) in dry THF (2 mL) in suspension of sodium hydride in dry THF (1 mL) (60% dispersion in mineral oil, 90 mg, 2.2 mmol) ) At 0-5 ° C. Stir at 0-5 ° C. for 5 minutes, then warm the reaction to room temperature for 10 minutes to complete the reaction. The reaction mixture is recooled to 0 ° C. and 5- (3-isoxazolyl) thiophene-2-sulfonyl chloride (273 mg, 1.1 mmol) in anhydrous THF (2 mL) is added slowly. Stirring is continued for 1 hour; During this period the reaction mixture slowly reaches ambient temperature. Remove THF under reduced pressure. The residue is dissolved in water (10 ml), the pH is adjusted to 2-3 by addition of concentrated HCl and extracted with methylene chloride (3 x 10 ml). The combined organic layers were dried over anhydrous magnesium sulfate and concentrated under reduced pressure to give N- (4-bromo-3-methyl-5-isoxazolyl) -5- (3-isoxazolyl) thiophen-2-sulfonamide To obtain. Recrystallization with hexane / ethyl acetate gives a pure material (160 mg, 41% yield) with a melting point of 120 to 123 ° C.
[587] Example 11
[588] N- (4-bromo-3-methyl-5-isoxazolyl) -2- (carmethoxy) thiophen-3-sulfonamide
[589] N- (4-bromo- in the same manner as described in Example 10 from 5-amino-4-bromo-3-methylisoxazole and 2- (carmethoxy) thiophen-3-sulfonyl chloride 3-Methyl-5-isoxazolyl) -2- (carmethoxy) thiophen-3-sulfonamide is prepared in 73% yield. Purification by recrystallization with ethyl acetate / hexanes affords a crystalline solid with a melting point of 198 to 200 ° C.
[590] Example 12
[591] N- (4-bromo-3-methyl-5-isoxazolyl) -2- (carboxyl) thiophene-3-sulfonamide
[592] N- (4-bromo-3-methyl-5-isoxazolyl) -2- (carmethoxy) thiophene-3-sulfonamide (Example 11) (1.5 g, 3.95 mmol) in methanol (10 ml) Dissolved in. Next, sodium hydroxide pellets (1 g, 25 mmol) and a few drops of water are added. The resulting solution is stirred at ambient temperature for 16 hours. Methanol is removed under reduced pressure. The residue is diluted with water and extracted with ethyl acetate (2 x 10 mL). The aqueous layer is acidified (pH = 2) with concentrated hydrochloric acid and extracted with ethyl acetate (2 × 60 mL). The combined organic layers are dried over anhydrous magnesium sulfate and filtered. Solvent was removed to yield N- (4-bromo-3-methyl-5-isoxazolyl) -2- (carbomethoxy) thiophen-3-sulfonamide (1.2 g, 82% yield) at melting points 188-194 ° C. ) Is purified by silica gel column chromatography using ethyl acetate as eluent.
[593] Example 13
[594] N- (3,4-dimethyl-5-isoxazolyl) -5-phenylthiophen-2-sulfonamide
[595] A. N- (3,4-dimethyl-5-isoxazolyl) -5-bromothiophene-2-sulfonamide
[596] 5-bromothiophene-2-sulfonyl chloride (2.75 g, 10 mmol) and 5-amino-3,4-dimethylisoxazole (1.07) in pyridine containing a catalytic amount of 4-dimethylaminopyridinepyridine (DMAP, 10 mg) g, 9.57 mmol) is stirred at room temperature for 3 hours. The solution is heated at 50 ° C. for an additional 1.5 hours to complete the reaction on TLC analysis. Pyridine is removed under reduced pressure, and the residue is extracted with ethyl acetate, then washed with 1N HCl (2 × 25 mL), water (1 × 25), brine solution (1 × 25 mL) and dried over magnesium sulfate. The solvent is evaporated to give a viscous brown gum which is flash chromatographed. Elution with 3% methanol hexanes yields 246 mg (10%) of pure sulfonamide.
[597] B. N- (methoxyethoxymethyl) -N- (3,4-dimethyl-5-isoxazolyl) -5-bromothiophene-2-sulfonamide
[598] N- (3,4-dimethyl-5-isoxazolyl) -5-bromothiophene-2-sulfonamide (680 mg, 2 mmol) in anhydrous THF (2 mL) was added sodium hydride (60% oil) in anhydrous THF (1 mL). 121 mg, 3 mmol) of dispersion. The resulting suspension is cooled to 0 ° C. and methoxyethoxymethyl chloride (334 mg, 2.68 mmol) is added dropwise by syringe. The solution is allowed to warm to room temperature and stirring is continued overnight. The solvent is evaporated to give an oil, extracted with ethyl acetate, washed with brine, dried over magnesium sulfate and evaporated. The residue was flash chromatographed on silica gel with 10-15% ethyl acetate / hexanes to give 480 mg (56%) of a colorless oil.
[599] C. N- (methoxyethoxymethyl) -N- (3,4-dimethyl-5-isoxazolyl) -5-phenylthiophen-2-sulfonamide
[600] Sodium carbonate (2 mL of 2M aqueous solution), followed by phenylboronic acid (86 mg, 0.71 mmol) in 2 mL of 95% ethanol, N- (methoxyethoxymethyl) -N- (3,4-dimethyl-5- in benzene anhydrous (4 mL). To a solution of isoxazolyl) -5-bromothiophene-2-sulfonamide (200 mg, 0.47 mmol) and tetrakis (triphenylphosphine) palladium (O) (23 mg, 0.02 mmol) is added under argon atmosphere. The mixture is refluxed for 12 h, then diluted with 5 mL of water and extracted with ethyl acetate (3 x 25 mL). The combined organic extracts are washed with brine (1 × 25 mL), dried and evaporated. The residue was flash chromatographed on silica gel with 25% ethyl acetate / hexanes to give 123 mg (62%) of sulfonamide as a colorless gum.
[601] D. N- (3,4-dimethyl-5-isoxazolyl) -5-phenylthiophene-2-sulfonamide
[602] HCl (3 ml of 3N aqueous solution) was added N- (methoxyethoxymethyl) -N- (3,4-dimethyl-5-isoxazolyl) -5-phenylthiophene-2-sulfonamide (3 mg in 3 mL of 95% ethanol). , 0.24 mmol), and the resulting mixture is refluxed for 6 hours. The mixture is then concentrated, diluted with 5 mL of water, neutralized with saturated aqueous sodium bicarbonate solution and acidified to pH 4 with glacial acetic acid. The mixture is extracted with ethyl acetate (2 × 25 mL) and the combined organic extracts are washed with brine (1 × 5 mL), dried and evaporated. The residue was flash chromatographed on silica gel with 2% MeOH / CHCl ₃ and further purified by reverse phase HPLC to give 33.4 mg (42%) of pure sulfonamide as melting point 176-178 ° C. as a white powder.
[603] Example 14
[604] N- (4-bromo-3-methyl-5-isoxazolyl) -5- (4-ethylphenyl) thiophene-2-sulfonamide
[605] A. N- (5-Bromothiophene-2-sulfonyl) -pyrrole
[606] Sodium hydride (60% oil dispersion, 191 mg, 4.78 mmol) is suspended in anhydrous tetrahydrofuran (2 mL) and the resulting turbid suspension is cooled to 0 ° C. in an ice bath. Pyrrole (385 mg, 5.75 mmol) in anhydrous tetrahydrofuran (2 mL) was added dropwise over 10 minutes. Remove the ice bath and stir the solution at room temperature (15 min) until gas stops dissipating, and pre-dissolved 5-bromothiophene-2-sulfonyl chloride (1.0 g) in tetrahydrofuran (4.0 mL) , 3.82 mmol) was added dropwise through a steel cannula. After stirring for 1 hour at room temperature, the mixture is filtered through celite. The filter pad is washed with tetrahydrofuran and the filtrate is evaporated to give a pale brown solid and recrystallized from methanol to give sulfonamide (821 mg, 74% yield) as a white powder.
[607] B. 4-ethylphenylboronic acid
[608] A solution of 1-bromo-4-ethyl benzene (2.0 g, 11 mmol) in anhydrous ether (5 mL) was added dropwise to magnesium pieces (311 g, 13 mmol) suspended in anhydrous ether. After the addition is complete, the suspension is refluxed for 15 minutes during which almost all magnesium reacts. The solution is then added to trimethyl borate (1.12 g, 11 mmol) pre-dissolved in ether (5 mL) at −78 ° C., warmed to room temperature and stirred for 90 minutes. The reaction is quenched by adding 10% aqueous HCl (2 mL) and the solution is extracted with ether. The combined ether extracts are extracted with 1M NaOH (2 × 20 ml), the aqueous extract is acidified to pH 2 with dilute HCl and extracted with ether (2 × 25 mL). The resulting combined ether extracts are washed once with water (10 mL), dried and evaporated to yield a white solid (676 mg, 38% yield) with a melting point of 138-140 ° C.
[609] C. N- [5- (4-ethylphenyl) thiophene-2-sulfonyl] pyrrole
[610] N- [5- (4-ethylphenyl) thiophen-2-sulfonyl] pyrrole was prepared in the same manner as described in Example 13C from 4-ethylphenylboronic acid and N- (5-bromothiophensulfonyl) pyrrole. To manufacture. Purification by column chromatography using 10% ethyl acetate / hexanes affords pure sulfonamide as a tan solid in 81% yield.
[611] D. 5-chlorosulfonyl-2- (4-ethylphenyl) thiophene
[612] A solution of N- [5- (4-ethylphenyl) thiophen-2-sulfonyl] pyrrole (100 mg, 0.32 mmol) and 6N sodium hydroxide (1 mL) in methanol (1.5 mL) was refluxed for about 6 hours. The solvent is evaporated and dried in vacuo to give an oil. Phosphorus oxychloride (258 ml, 2.52 mmol) and phosphorus pentachloride (131 mg, 0.63 mmol) are added to the oil and the resulting brown suspension is heated at 50 ° C. for 3 hours. The resulting clear brown solution is carefully added to about 20 mL of crushed ice and then extracted with ethyl acetate (3 × 25 mL). The combined organic layers are washed with brine (2 × 5 mL), dried (MgSO ₄ ) and evaporated to give an oily residue. Flash chromatography on silica gel using 2% ethyl acetate / hexanes gave pure sulfonyl chloride (53 mg, 59%) as a pale yellow oil.
[613] E. N- (4-Bromo-3-methyl-5-isoxazolyl) -5- (4-ethylphenyl) thiophene-2-sulfonamide
[614] N- (4-bromo-3-methyl-5-isoxazolyl) -5- (4-ethylphenyl) thiophene-2-sulfonamide was prepared in the same manner as described in Example 10. 5-chlorosulfonyl-2- (4-ethylphenyl) thiophene (47.1 mg, 11.16 mmol) was reacted with 5-amino-4-bromo-3-methyl isoxazole (29 mg, 0.16 mmol), 10% Flash chromatography using MeOH / CHCl ₃ affords a light brown solid (46 mg, 66% yield) at a melting point of 172 ° C. to 175 ° C.
[615] Example 15
[616] N- (4-bromo-3-methyl-5-isoxazolyl) -4-phenethylthiophene-2-sulfonamide
[617] N- (4-bromo-3-methyl-5-isoxazolyl) -4-phenethylthiophene-2-sulfonamide to 5-amino-4-bromo-3-methylisoxazole and 4-pen Prepared from ethyl-2-thiophensulfonyl chloride in the same manner as described in Example 10 in 32% yield. It is purified by HPLC (5% CH ₃ CN to 100% CH ₃ CN over 30 minutes) to give a gum.
[618] Example 16
[619] N- (4-bromo-3-methyl-5-isoxazolyl) -2- [N- (3-carboxyphenylaminocarbonyl] thiophene-3-sulfonamide
[620] Et ₃ N (2.27 ml, 16. mmol), ethyl 3-aminobenzoate (836 ml, 5.44 mmol) and phosphonitrile chloride trimer (1.89 g, 5.44 mmol) were added to N- (4 in anhydrous THF (20 mL). Add sequentially to a solution of bromo-3-methyl-5-isoxazolyl) -2- (carbonyl) thiophene-3-sulfonamide (Example 12) (1 g, 2.27 mmol). The reaction is stirred for 1 hour at room temperature and cooled. The reaction was quenched by addition of water (5 ml). The resulting solution is concentrated in rotabac. The residue is diluted with EtOAc and washed with 2N HCl (2 × 150 mL). The organic layer is dried (MgSO ₄ ). The solid is filtered off and the filtrate is concentrated. The residue is treated with 1N NaOH (200 mL) and stirred at 0 ° C. for 15 minutes. The mixture is acidified with concentrated HCl to bring the pH to about 1. The resulting yellow precipitate was recrystallized from CH ₃ CN / H ₂ O to give N- (4-bromo-3-methyl-5-isoxazolyl) -2- [N- (3-carboxy) at a melting point of 183 to 185 ° C. Phenylaminocarbonyl] thiophene-3-sulfonamide (153 mg, 11.6%) is obtained as a yellow powder.
[621] Example 17
[622] N- (4-bromo-5-methyl-3-isoxazolyl) -5- (4-methylphenyl) thiophene-2-sulfonamide
[623] A. N- [5- (4-methylphenyl) thiophene-2-sulfonyl] pyrrole
[624] N- [5- (4-methylphenyl) thiophen-2-sulfonyl] pyrrole was described in Example 13C using 4-methyl-phenylboronic acid and N- (5-bromothiophensulfonyl) pyrrole. Prepare in the same way. Purification by column chromatography using 2% ethyl acetate / hexanes affords N- [5- (4-methylphenyl) thiophen-2-sulfonyl] pyrrole as a pale yellow solid in 77% yield.
[625] B. 2-chlorosulfonyl-5- (4-methylphenyl) thiophene
[626] 2-Chlorosulfonyl-5- (4-methylphenyl) thiophene is prepared in the same manner as described in Example 14D using N- [5- (4-methylphenyl) thiophen-2-sulfonyl] pyrrole. . Purification by column chromatography using 2% ethyl acetate / hexanes affords 2-chlorosulfonyl-5- (4-methylphenyl) thiophene as a pale yellow powder in 61% yield.
[627] C. N- (4-bromo-3-methyl-5-isoxazolyl) -5- (4-methylphenyl) thiophene-2-sulfonamide
[628] N- (4-bromo-3-methyl-5-isoxazolyl) -5- (4-methylphenyl) thiophene-2-sulfonamide was prepared in the same manner as described in Example 10. 2-chlorosulfonyl-5- (4-methylphenyl) thiophene (100 mg, 0.37 mmol) was reacted with 5-amino-4-bromo-3-methylisoxazole (65 mg, 0.37 mmol) and 10% MeOH Column chromatography with / CHCl ₃ gives the final product (96 mg, 63% yield) as a pale yellow solid at melting point 175 ° C.
[629] Example 18
[630] N- (4-bromo-3-methyl-5-isoxazolyl) -5- (benzyloxymethyl) thiophene-2-sulfonamide
[631] A. 2- (benzyloxymethyl) thiophene
[632] Sodium hydride (0.41 mg, 20 mmol) is added to 2-thiophene methanol (2.0 g, 0.18 mmol) in THF (20 mL) at -40 ° C. The reaction is stirred at −40 ° C. for 25 minutes, then pure benzylbromide (3.6 g, 20 mmol) is added by syringe. The solution is stirred at −40 ° C. for 0.5 hour and then at room temperature for 1 hour. THF is evaporated and the remaining residue is dissolved in ether (-50 ml). The organic solution is washed with water (1 × 10 mL), brine (1 × 10 mL) and dried over MgSO ₄ . The solvent is evaporated to give an oil which is purified by column chromatography using 1% ether-hexane to give thiophene (2.6 g, 78% yield) as a pale yellow oil.
[633] B. 2-chlorosulfonyl-5- (benzyloxymethyl) thiophene
[634] 2-Chlorosulfonyl-5- (benzyloxymethyl) thiophene is prepared from 2- (benzyloxymethyl) thiophene (1.0 g, 5.25 mmol) in the same manner as described in Example 17A. It is purified by column chromatography using 2.5% ethyl acetate / hexanes to give pure thiophene (520 mg, 32% yield) as a brown oil.
[635] C. N- (4-Bromo-3-methyl-5-isoxazolyl) -5- (benzyloxymethyl) thiophene-2-sulfonamide
[636] N- (4-bromo-3-methyl-5-isoxazolyl) -5- (benzyloxymethyl) thiophene-2-sulfonamide is 2-chlorosulfonyl-5- (benzyloxymethyl) thiophene ( 520 mg, 1.72 mmol) and 5-amino-4-bromo-3-methyl isoxazole (319 mg, 1.8 mmol) in the same manner as described in Example 10. Purification by column chromatography using 10% MeOH / CHCl ₃ gave 238 mg of N- (4-bromo-3-methyl-5-isoxazolyl) -5- (benzyloxymethyl) thiophen-2-sulfonamide. Obtained as a brown semisolid (31% yield, melting point 92 ° C.).
[637] Example 19
[638] N- (4-bromo-3-methyl-5-isoxazolyl) -3- [3,4- (methylenedioxy) phenyl] thiophene-2-sulfonamide
[639] A. 3-bromothiophene-2-sulfonyl chloride
[640] Chlorosulfonic acid (20 ml, 300 mmol) is added to a solution of 3-bromothiophene (8.15 g, 50 mmol) in methylene chloride (50 ml) at -78 ° C for 20 minutes. After the addition is complete, the cold bath is removed and stirred at ambient temperature for 1 hour. The reaction mixture is added dropwise to carefully crushed ice (100 g). The mixture is extracted with methylene chloride (2 x 100 mL). The combined organic layers are dried over MgSO ₄ and evaporated. The crude product was purified by flash chromatography on silica gel using hexane as eluent to give 3-bromothiophene-2-sulfonyl chloride (4 g, 30% yield) and 4-bromothiophene-2-sulfonyl chloride ( 200 mg, <1%).
[641] B. N- (3-bromothiophene-2-sulfonyl) pyrrole
[642] N- (3-bromothiophene-2-sulfonyl) pyrrole is prepared in the same manner as described in Example 14A by reacting 3-bromothiophene-2-sulfonyl chloride with pyrrole for 16 hours. N- (3-bromothiophen-2-sulfonyl) pyrrole is obtained in a yield of 54%.
[643] C. N-{[3- (3,4-methylenedioxy) phenyl] thiophene-2-sulfonyl} pyrrole
[644] N-{[3- (3,4-methylenedioxy) phenyl] thiophene-2-sulfonyl} pyrrole can be used to determine 3,4-methylenedioxyphenylboronic acid and N- (3-bromothiophene-2-sulfur Prepared in the same manner as described in Example 13C using phonyl) pyrrole. The crude product was purified by flash column chromatography using silica gel with 2% EtOAc in hexanes to afford 90% of N-{[3- (3,4-methylenedioxy) phenyl] thiophene-2-sulfonyl} pyrrole. Obtained in the yield.
[645] D. 2-chlorosulfonyl-3- [3,4- (methylenedioxy) phenyl] thiophene
[646] 2-chlorosulfonyl-3- [3,4- (methylenedioxy) phenyl] thiophene can be obtained by N-{[3- (3,4-methylenedioxy) phenyl] thiophene-2-sulfonyl} pyrrole. Prepared in the same manner as described in Example 18B, wherein the sulfonamide was subjected to basic hydrolysis to obtain sodium sulfonate (100% yield), followed by conversion of the salt to the corresponding sulfonyl chloride to obtain 34% of the final product. Obtained in the yield.
[647] E. N- (4-Bromo-3-methyl-5-isoxazolyl) -3- [3,4- (methylenedioxy) phenyl] thiophene-2-sulfonamide
[648] N- (4-bromo-3-methyl-5-isoxazolyl) -3- [3,4- (methylenedioxy) phenyl] thiophene-2-sulfonamide is 2-chlorosulfonyl-3- [ Reaction of 3,4- (methylenedioxy) phenyl] thiophene with 5-amino-4-bromo-3-methylisoxazole, yielding 60% yield (melting point 183 to 183) in the same manner as described in Example 9. 186 ° C.).
[649] Example 20
[650] N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(2-chloro-3,4-methylenedioxy) phenoxymethyl] thiophene-3-sulfonamide
[651] A. N- {2-[(3,4-methylenedioxy) phenoxymethyl] thiophene-3-sulfonyl} pyrrole
[652] Sodium hydride (100 mg, 5 mmol) is added to a stirred solution of 3,4-methylenedioxyphenol (0.607 g, 4.5 mmol) in DMF (anhydrous, 5 ml) with stirring at 0 ° C. under a nitrogen atmosphere. The reaction mixture is allowed to warm to room temperature and stirring is continued for 1 hour. The reaction mixture is cooled to 0 ° C. and N-[(2-bromomethyl) thiophen-3-sulfonyl] pyrrole is added. Continue stirring at room temperature for 16 hours. The reaction mixture is diluted with water (100 ml), extracted with ethyl acetate (2 × 50 mL) and washed with 1N NaOH (2 × 25 mL) to remove phenol derivatives. The mixture was dried over MgSO ₄ and concentrated to give N- {2-[(3,4-methylenedioxy) phenoxymethyl] thiophen-3-sulfonyl} pyrrole, which was recrystallized using hexane / EtOAc. (1.0 g, 92% yield).
[653] B. 3-chlorosulfonyl-2-[(2-chloro-3,4-methylenedioxy) phenoxymethyl] thiophene
[654] 3-chlorosulfonyl-2-[(2-chloro-3,4-methylenedioxy) phenoxymethyl] thiophene is N- {2-[(3,4-methylenedioxy) phenoxymethyl] thiophene In the same manner as described in Example 15E, basic hydrolysis (using potassium hydroxide in iso-propanol) with potassium sulfonate using -3-sulfonyl} pyrrole and then converting the salt to the corresponding sulfonyl chloride Prepare in 50% total yield.
[655] C. N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[(2-chloro-3,4-methylenedioxy) phenoxymethyl] thiophene-3-sulfonamide
[656] N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(2-chloro-3,4-methylenedioxy) phenoxymethyl] thiophene-3-sulfonamide is 3-chloro Described in Example 9 by reacting sulfonyl-2-[(2-chloro-3,4-methylenedioxy) phenoxymethyl] thiophene with 5-amino-4-bromo-3-methylisoxazole Prepared in the same manner as in the 47% yield (melting point 152 to 154 ℃).
[657] Example 21
[658] N- (4-bromo-3-methyl-5-isoxazolyl) -2- [trans-3,4- (methylenedioxy) cinnamyl] thiophene-3-sulfonamide
[659] A. diethyl 2- {3-[(N-pyrrolyl) sulfonyl] thienylmethyl} phosphonate
[660] N- [2-bromomethyl) thiophene-3-sulfonyl] pyrrole (0.915 g, 3 mmol) is suspended in triethyl phosphite (5 ml) and heated to 140 ° C. for 1 hour with stirring under nitrogen atmosphere. Excess triethyl phosphate was removed under reduced pressure and the residue was dried under vacuum to afford diethyl 2- {3-[(N-pyrrolyl) sulfonyl] thienylmethyl} phosphonate (0.9 g, 83% yield). To obtain.
[661] B. N- {2- [trans-3,4- (methylenedioxy) cinnamyl] thiophene-3-sulfonyl} pyrrole
[662] Sodium hydride (200 mg, 60% dispersion) with a stirred solution of diethyl 2- {3-[(N-pyrrolyl) sulfonyl] thienylmethyl} phosphonate (900 mg, 2.48 mmol) in anhydrous THF (10 ml) To two portions with stirring at 0 ° C. The reaction mixture is continued to stir at room temperature for 1 hour, then piperonal (600 mg) is added. Continue stirring for 12 hours. The mixture is diluted with water (100 ml) and extracted with methylene chloride (2 x 50 mL). The combined organic layers were dried over MgSO ₄ , evaporated and the residue was purified by flash chromatography using silica gel with 0.5% ethyl acetate in hexanes on silica gel to give N- {2- [trans-3,4- (methylenedioxy) Cinnamic] thiophen-3-sulfonyl} pyrrole (750 mg, 84% yield) is obtained.
[663] C. 3-chlorosulfonyl-2- [trans-3,4- (methylenedioxy) cinnamyl] thiophene
[664] 3-chlorosulfonyl-2- [trans-3,4- (methylenedioxy) cinnamyl] thiophene is N- {2- [trans-3,4- (methylenedioxy) cinnamyl] thiophen-3 Total hydrolysis in the same manner as described in Example 15E by basic hydrolysis (using isopropanol and potassium hydroxide) from sulfonyl} pyrrole to the corresponding potassium sulfonate (100%) followed by conversion of the salt to the corresponding sulfonyl chloride. Prepared in yield 31%.
[665] D. N- (4-Bromo-3-methyl-5-isoxazolyl) -2- [trans-3,4- (methylenedioxy) cinnamyl] thiophene-3-sulfonamide
[666] N- (4-bromo-3-methyl-5-isoxazolyl) -2- [trans-3,4- (methylenedioxy) cinnamyl] thiophene-3-sulfonamide is 3-chlorosulfonyl- Prepared in the same manner as described in Example 9 by reacting 2- [trans-3,4- (methylenedioxy) cinnamil] thiophene with 5-amino-4-bromo-3-methylisoxazole . The crude product is purified by HPLC to give 33% yield (melting point 147-149 ° C.).
[667] Example 22
[668] N- (4-bromo-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) phenethyl] thiophen-3-sulfonamide
[669] A. N- {2- [3,4- (methylenedioxy) phenethyl] thiophen-3-sulfonyl} pyrrole
[670] 10% solution of ethyl acetate (15 ml) of N- {2- [trans-3,4- (methylenedioxy) cinnamyl] thiophene-3-sulfonyl} pyrrole (Example 21B, 0.6 g, 1.67 mmol) Catalytic hydrogenation with Pd-C (100 mg) is carried out at 55 psi for 14 hours. The catalyst is filtered off and the filtrate is concentrated to give N- {2- [3,4- (methylenedioxy) phenethyl] thiophene-3-sulfonyl} pyrrole (0.55 g, 91% yield).
[671] B. 3-chlorosulfonyl-2- [3,4- (methylenedioxy) phenethyl] thiophene
[672] 3-chlorosulfonyl-2- [3,4- (methylenedioxy) phenethyl] thiophene is N- {2- [3,4- (methylenedioxy) phenethyl] thiophene-3-sulfonyl} In the same manner as described in Example 15E, pyrrole was used to basicly hydrolyze sulfonamide to potassium sulfonate salt (93% yield) (isopropanol and potassium hydroxide) and then convert this salt to the corresponding sulfonyl chloride. The yield is 42%.
[673] C. N- (4-Bromo-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) phenethyl] thiophene-3-sulfonamide
[674] N- (4-bromo-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) phenethyl] thiophene-3-sulfonamide was the same as described in Example 10. It is prepared by the method. 3-chlorosulfonyl-2- [3,4- (methylenedioxy) phenethyl] thiophene is reacted with 5-amino-4-bromo-3-methylisoxazole and the crude product is purified by HPLC. N- (4-bromo-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) phenethyl] thiophene-3-sulfonamide is obtained in a yield of 30% ( Melting point 180 ° (decomposition)).
[675] Example 23
[676] N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(4-methyl) (cinnamil)] thiophene-3-sulfonamide
[677] A. N- [2- (4-Methyl-trans-styryl) -3-sulfonyl] pyrrole
[678] N- [2- (4-methyl-trans-styryl) -3-sulfonyl] pyrrole is diethyl {3-[(N-pyrrolylsulfonyl) thien-2- [yl] methylphosphonate and 4- Prepared in the same manner as described in Example 21B using methylbenzaldehyde in 30% yield.
[679] B. 2- (4-Methyl-trans-styryl) thiophene-3-sulfonyl chloride
[680] 2- (4-Methyl-trans-styryl) thiophene-3-sulfonyl chloride was converted to the corresponding sulfonic acid using N- [2- (4-methyl-trans-styryl) -3-sulfonyl} pyrrole. Basic hydrolysis with sodium (using ethanol and sodium hydroxide) followed by conversion to the corresponding sulfonyl chloride was prepared in 13% yield in the same manner as described in Example 15E.
[681] C. N- (4-Bromo-3-methyl-5-isoxazolyl) -2- (4-methyl-trans-styryl) thiophene-3-sulfonamide
[682] N- (4-Bromo-3-methyl-5-isoxazolyl) -2- (4-methyl-trans-styryl) -thiophene-3-sulfonamide is 2- (4-methyl-trans-sty Reel) thiophen-3-sulfonyl chloride was prepared in the same manner as described in Example 10 by reacting with 5-amino-4-bromo-3-methylisoxazole. The crude product is purified by HPLC and recrystallized to yield 34% yield (melting point 101-105 ° C.).
[683] Example 24
[684] N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(4-methyl) phenethyl] thiophen-3-sulfonamide
[685] A. N- {2-[(4-methyl) phenethyl] thiophen-3-sulfonyl} pyrrole
[686] N- {2-[(4-methyl) phenethyl] thiophene-3-sulfonyl} pyrrole was prepared in the same manner as described in Example 22A, with N- [2- (4-methyl-trans-styryl)- 3-sulfonyl} pyrrole is prepared by catalytic hydrogenation in a yield of 80%.
[687] B. 2-[(4-methyl) phenethyl] thiophene-3-sulfonyl chloride
[688] 2-[(4-methyl) phenethyl] thiophene-3-sulfonyl chloride was used to prepare potassium sulfonamide using N- {2-[(4-methyl) phenethyl] thiophene-3-sulfonyl} pyrrole. Prepared in 51% yield in the same manner as described in Example 15E by basic hydrolysis (KOH / ethanol) with salts followed by conversion to the corresponding sulfonyl chloride.
[689] C. N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[(4-methyl) phenethyl] thiophen-3-sulfonamide
[690] N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(4-methyl) phenethyl] thiophen-3-sulfonamide is 2-[(4-methyl) phenethyl] ti Prepared in a yield of 52% in the same manner as described in Example 10 using fen-3-sulfonyl chloride and 5-amino-4-bromo-3-methylisoxazole.
[691] Example 25
[692] N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(4-methylphenoxy) methyl] thiophene-3-sulfonamide
[693] A. N- {2-[(4-methylphenoxy) methyl] thiophen-3-sulfonyl} pyrrole
[694] N- {2-[(4-methylphenoxy) methyl] thiophen-3-sulfonyl} pyrrole was treated with N- [2-bromomethyl) thiophen-3-sul by the same method as described in Example 20A. Ponyl] pyrrole is reacted with 4-methylphenol to make 81% yield.
[695] B. 2-[(4-methylphenoxy) methyl] thiophene-3-sulfonyl chloride
[696] 2-[(4-methylphenoxy) methyl] thiophene-3-sulfonyl chloride was subjected to basic hydrolysis using N- {2-[(4-methylphenoxymethyl] thiophene-3-sulfonyl} pyrrole (NaOH / EtOH), followed by conversion to the corresponding sulfonyl chloride, to give a yield of 46% in the same manner as described in Example 15E.
[697] C. N- (4-Bromo-3-methyl-5-isoxazolyl) -2-[(4-methylphenoxy) methyl] thiophene-3-sulfonamide
[698] N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(4-methylphenoxy) methyl] thiophene-3-sulfonamide was prepared in 3-chloro as described in Example 10. A sulfonyl-2-[(4-methylphenoxy) methyl] thiophene is reacted with 5-amino-4-bromo-3-methylisoxazole to give a yield of 64% (melting point 128-130 ° C.). .
[699] Example 26
[700] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methyl] phenylaminocarbonyl-3-thiophenesulfonamide
[701] A. (3,4-methylenedioxy) -6-methylaniline
[702] Nitric acid (70%, 5 ml) is added dropwise to a solution of (3,4-methylenedioxy) toluene (5 ml) in acetic acid (20 ml) cooled with a cold water bath. The mixture is stirred for 45 minutes. As a workup, water (100 ml) is added and the resulting yellow precipitate is filtered off and washed with water until the aqueous filtrate is colorless. The yellow solid is dissolved in EtOAc (250 ml), dried (MgSO ₄ ) and the solid is filtered. The filtrate is subjected to catalytic hydrogenation (10% Pd / C, 1 atm) for 12 hours. The reaction mixture is filtered to remove the catalyst and the filtrate is concentrated in rotabac to give (3,4-methylenedioxy) -6-methylaniline as off-brown solid (5.49 g, 87% yield).
[703] B. N- (4-Chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methyl] phenylaminocarbonyl-3-thiophenesulfonamide
[704] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methyl] phenylaminocarbonyl-3-thiophenesulfonamide is described in Example 3. Prepared using (3,4-methylenedioxy) -6-methylaniline in the same manner as described in. The crude product was purified by preparative HPLC to give N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methyl] phenylaminocarbonyl-3- Thiophensulfonamide is obtained as a yellow solid (45% yield, melting point 60-62 ° C.).
[705] Example 27
[706] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-methoxycarbonyl-2,4,6-trimethyl) phenylaminocarbonyl-3-thiophenesulfonamide
[707] A. Methyl 3-amino-2,4,6-trimethylbenzoate
[708] Methyl 3-amino-2,4,6-trimethylbenzoate is prepared in the same manner as (3,4-methylenedioxy) -6-methylaniline (see Example 26).
[709] B. N- (4-Chloro-3-methyl-5-isoxazolyl) -2- (3-methoxycarbonyl-2,4,6-trimethyl) phenylaminocarbonyl-3-thiophenesulfonamide
[710] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-methoxycarbonyl-2,4,6-trimethyl) phenylaminocarbonyl-3-thiophenesulfonamide replaces THF Was prepared in the same manner as described in Example 3 except that the reaction was heated at 80 ° C. for 5 hours. The crude product was purified by preparative HPLC to give N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-methoxycarbonyl-2,4,6-trimethyl) phenylaminocarbonyl-3 -Thiophenesulfonamide is obtained as off-white powder (48 mg, 1% yield, melting point 66-70 ° C.).
[711] Example 28
[712] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,4,6-trimethyl) phenylacetyl-3-thiophensulfonamide
[713] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,4,6-trimethyl) phenylacetyl-3-thiophenesulfonamide is a mixture of 2,4,6-trimethylbenzyl chloride and N -(4-chloro-3-methyl-5-isoxazolyl) -2- (N-methyl-N'-methoxy) aminocarbonyl-3-thiophenesulfonamide as described in Example 5 Prepare in the same way. The crude product was purified by flash column chromatography (1% methanol in eluent CH ₂ Cl ₂ ) to give N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,4,6-trimethyl) Phenylacetyl-3-thiophenesulfonamide is obtained as a solid (31% yield, melting point 42-46 ° C.).
[714] Example 29
[715] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,4,6-trimethyl) phenylaminocarbonyl-3-thiophenesulfonamide
[716] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,4,6-trimethyl) phenylaminocarbonyl-3-thiophenesulfonamide was prepared in the same manner as described in Example 3. To manufacture. The crude product was purified by preparative HPLC to give N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,4,6-trimethyl) phenylaminocarbonyl-3-thiophenesulfonamide Obtained as a powder (410 mg, 30% yield, melting point 45 to 48 ° C.).
[717] Example 30
[718] N- (3,4-dimethyl-5-isoxazolyl) -2- (2,4-dimethyl) phenylacetyl-3-thiophenesulfonamide
[719] N- (3,4-dimethyl-5-isoxazolyl) -2- (2,4-dimethyl) phenylacetyl-3-thiophenesulfonamide is a mixture of 2,4-dimethylbenzyl chloride and N- (3,4- Prepared in the same manner as described in Example 5 using dimethyl-5-isoxazolyl) -2- (N-methyl-N'-methoxy) aminocarbonyl-3-thiophensulfonamide. The crude product was purified by flash column chromatography (1% methanol in eluent CH ₂ Cl ₂ ) and further purified by preparative HPLC to give N- (3,4-dimethyl-5-isoxazolyl) -2- (2, 4-Dimethyl) phenylacetyl-3-thiophenesulfonamide is obtained as a semisolid (34% yield).
[720] Example 31
[721] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,4-dimethyl) phenylacetyl-3-thiophenesulfonamide
[722] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,4-dimethyl) phenylacetyl-3-thiophenesulfonamide is a mixture of 2,4-dimethylbenzyl chloride and N- (4- Prepared in the same manner as described in Example 5 using chloro-3-methyl-5-isoxazolyl) -2- (N-methyl-N'-methoxy) aminocarbonyl-3-thiophenesulfonamide do. The crude product was purified by flash column chromatography (1% methanol in eluent CH ₂ Cl ₂ ) to give N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,4-dimethyl) phenylacetyl 3-thiophenesulfonamide is obtained as a solid (52% yield, melting point 48-54 ° C.).
[723] Example 32
[724] N- (4-bromo-3-methyl-5-isoxazolyl) -2- (2,4-dimethyl) phenylacetyl-3-thiophenesulfonamide
[725] N- (4-bromo-3-methyl-5-isoxazolyl) -2- (2,4-dimethyl) phenylacetyl-3-thiophenesulfonamide is a mixture of 2,4-dimethylbenzyl chloride and N- (4 The same method as described in Example 5 using bromo-3-methyl-5-isoxazolyl) -2- (N-methyl-N'-methoxy) aminocarbonyl-3-thiophenesulfonamide To manufacture. The crude product was purified by flash column chromatography (1% methanol in eluent CH ₂ Cl ₂ ) and further purified by preparative HPLC to give N- (4-bromo-3-methyl-5-isoxazolyl) -2- (2,4-dimethyl) phenylacetyl-3-thiophenesulfonamide is obtained as a solid (28% yield, melting point 58-63 ° C.).
[726] Example 33
[727] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3,5-dimethyl) phenylacetyl-3-thiophenesulfonamide
[728] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3,5-dimethyl) phenylacetyl-3-thiophenesulfonamide is a mixture of 3,5-dimethylbenzyl bromide and N- (4- Prepared in the same manner as described in Example 5 using chloro-3-methyl-5-isoxazolyl) -2- (N-methyl-N'-methoxy) aminocarbonyl-3-thiophenesulfonamide do. The crude product was purified by flash column chromatography (2% methanol in eluent CH ₂ Cl ₂ ) to give N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3,5-dimethyl) phenylacetyl 3-thiophenesulfonamide is obtained as a solid (57% yield, melting point 45-50 [deg.] C.).
[729] Example 34
[730] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,5-dimethyl) phenylacetyl-3-thiophenesulfonamide
[731] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,5-dimethyl) phenylacetyl-3-thiophenesulfonamide is a mixture of 2,5-dimethylbenzyl chloride and N- (4- Prepared in the same manner as described in Example 5 using chloro-3-methyl-5-isoxazolyl) -2- (N-methyl-N'-methoxy) aminocarbonyl-3-thiophenesulfonamide do. The crude product was purified by flash column chromatography (2% methanol in eluent CH ₂ Cl ₂ ) to give N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,5-dimethyl) phenylacetyl 3-thiophenesulfonamide is obtained as a solid (33% yield, melting point 72-76 ° C.).
[732] Example 35
[733] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6- (2-acetoxyethyl) phenylaminocarbonyl-3-thiophenesulfone amides
[734] A. 2- (3,4-methylenedioxy) phenyl-1-ethanol
[735] To a solution of 2- (3,4-methylenedioxy) phenylacetic acid (5 g, 25.75 mmol) in dry THF (20 ml) is added BH ₃ .THF (40 ml, 1.0 M in THF) at 0 ° C. The mixture is stirred at rt for 1 h. As a workup, THF is evaporated in the rotabac. The residue is treated with water (100 ml), acidified and extracted with ether (2 × 100 ml). The solvent is removed under reduced pressure to give 2- (3,4-methylenedioxy) phenyl-1-ethanol as an oil (4.7 g, 98% yield).
[736] B. 1-acetoxy-2-[(3,4-methylenedioxy) phenyl] ethane
[737] Acetic anhydride is added to a stirred solution of 2- (3,4-methylenedioxy) phenyl-1-ethanol (1.68 g, 10 mmol) in anhydrous pyridine and the resulting reaction mixture is stirred at 80 ° C. for 1 hour. The reaction mixture is poured into iced water and extracted with ether (2 x 75 ml). The combined ether extracts are washed with water (2 × 50 ml), 5% HCl (2 × 50 ml) and 5% NaHCO ₃ (2 × 50 ml). The organic layer is dried over magnesium sulfate and the solvent is removed under reduced pressure to give 1-acetoxy-2-[(3,4-methylenedioxy) phenyl] ethane as a solid (1.7 g, 81% yield).
[738] C. 1-acetoxy-2-[(3,4-methylenedioxy) -6-nitrophenyl] ethane
[739] To a stirred solution of 1-acetoxy-2-[(3,4-methylenedioxy) phenyl] ethane (1.7 g, 8.09 mmol) in acetic acid (10 ml) was added dropwise concentrated HNO ₃ (4.5 ml). It is stirred for 30 minutes at room temperature. The reaction mixture is poured into water (100 ml). The precipitated solid is filtered, washed with water and dried under high vacuum to give 1-acetoxy-2-[(3,4-methylenedioxy) -6-nitrophenyl] ethane (1.8 g, 88% yield). .
[740] D. 1-acetoxy-2-[(3,4-methylenedioxy) -6-aminophenyl] ethane
[741] 10% palladium on carbon (100 mg) was used in a solution of 1-acetoxy-2-[(3,4-methylenedioxy) -6-nitrophenyl] ethane (0.8 g, 3.13 mmol) in ethyl acetate (25 ml) Catalytic hydrogenation was carried out for 30 minutes at 50 psi. The catalyst is filtered off and the solvent is removed under reduced pressure to give 1-acetoxy-2-[(3,4-methylenedioxy) -6-aminophenyl] ethane (0.69 g, 98% yield) as a solid.
[742] E. N- (4-Chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6- (2-acetoxyethyl) phenylaminocarbonyl-3-ti Offensulfonamide
[743] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6- (2-acetoxyethyl) phenylaminocarbonyl-3-thiophenesulfone Amides are prepared in the same manner as described in Example 16. The crude product was purified by preparative HPLC to give N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6- (2-acetoxyethyl) phenylamino Carbonyl-3-thiophenesulfonamide is obtained as a pale yellow powder (12% yield, melting point 78-82%).
[744] Example 36
[745] Other compounds prepared by the above methods or conventional modifications thereof include, but are not limited to:
[746] N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(4-methoxyphenoxy) carbonyl] thiophene-3-sulfonamide, N- (4-bromo-3- Methyl-5-isoxazolyl) -2-[(4-methoxyphenoxy) carbonyl] thiophene-3-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl)- 3-[(4-methylphenoxy) methyl] thiophene-2-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(4-methylphenoxy) methyl ] Thiophene-3-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-methyl-trans-styryl) thiophene-2-sulfonamide, N- (4-Bromo-3-methyl-5-isoxazolyl) -3- (4-methylphenethyl) thiophene-2-sulfonamide, N- (4-bromo-3-methyl-5-isox Zolyl) -2-[(4-methylphenyl) acetyl] thiophene-3-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(3-methoxyphenyl) Acetyl] thiophene-3-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-methylphenethyl) -5- (4-tolyl) thiophene-2 -Sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-methylbenzyl) -5- (4-tolyl) Offen-2-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-methyl-trans-styryl) -5- (4-tolyl) thiophen-2 -Sulfonamide, N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) benzyl] thiophene-3-sulfonamide, N- (4-chloro -3-methyl-5-isoxazolyl) -2-[(5-methyl-3-isoxazolyl) aminocarbonyl] thiophene-3-sulfonamide, N- (4-bromo-3-methyl- 5-isoxazolyl) -2-[(3-hydroxyl-6-pyridazinyl) aminocarbonyl] thiophene-3-sulfonamide, N- (4-bromo-3-methyl-5-isoxane Zolyl) -3-{[3,4- (methylenedioxy) phenoxy] methyl} thiophene-2-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -2- [(4-methyl) (cinnamil)] thiophene-3-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3- [3,4- (methylenedioxy) Phenethyl] thiophene-2-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3- [3,4- (methylenedioxy) -trans-styryl] thiophene -2-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(4-meth ) Phenethyl] thiophene-3-sulfonamide, N- (3,4-dimethyl-5-isoxazolyl) -2- (4-tolylacetylphenyl) thiophene-3-sulfonamide, N- (3, 4-dimethyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) phenylacetyl] thiophene-3-sulfonamide, N- (4-chloro-3-methyl-5-isoxazolyl ) -2- [2-hydroxy-4-methylphenyl) aminocarbonyl] thiophen-3-sulfonamide and compounds other than those set forth in Tables 1a to 1r not specifically exemplified herein.
[747] For example, N- (4-bromo-3-methyl-5-isoxazolyl) -3- [2-methyl-4,5- (methylenedioxy) cinnamyl] thiophene-2-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3- [2- (hydroxymethyl) -4,5- (methylenedioxy) cinnamyl] thiophene-2-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3- {2-[(tetrahydro-4H-pyran-2-yloxy) methyl] -4,5- (methylenedioxy) Cinnamic} thiophene-2-sulfonamide and N- (4-bromo-3-methyl-5-isoxazolyl) -3- (2,4-dimethylcinnamil) thiophene-2-sulfonamide Prepared by the same method as-(4-bromo-3-methyl-5-isoxazolyl) -3- [3,4- (methylenedioxy) -trans-styryl] thiophene-2-sulfonamide. N- (4-bromo-3-methyl-5-isoxazolyl) -3- [2-methyl-4,5- (methylenedioxy) phenethyl] thiophene-2-sulfonamide and N- (4 -Bromo-3-methyl-5-isoxazolyl) -2- (2,4,6-trimethylphenethyl) thiophen-3-sulfonamide with N- (4-bromo-3-methyl-5- Prepared in the same manner as isoxazolyl) -2-[(4-methyl) phenethyl] thiophen-3-sulfonamide (see Example 24). N- (4-bromo-3-methyl-5-isoxazolyl) -3-{[2-propyl-4,5- (methylenedioxy) phenoxy] methyl} thiophene-2-sulfonamide N -(4-bromo-3-methyl-5-isoxazolyl) -3-[(4-methylphenoxy) methyl] thiophene-2-sulfonamide and N- (4-bromo-3-methyl- Prepared in the same manner as 5-isoxazolyl) -3-{[3,4- (methylenedioxy) phenoxy] methyl} thiophene-2-sulfonamide. N- (4-bromo-3-methyl-5-isoxazolyl) -2- [2-methyl-4,5- (methylenedioxy) phenethyl] thiophene-3-sulfonamide in N- (4 -Bromo-3-methyl-5-isoxazolyl) -2-[(3,4-methylenedioxy) phenethyl] thiophene-3-sulfonamide. Compounds such as N- (4-bromo-3-methyl-5-isoxazolyl) -3- (2-tolyl) thiophene-2-sulfonamide, N- (4-bromo-3- Methyl-5-isoxazolyl) -3- (3-tolyl) thiophene-2-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3- (2-tolyl) Thiophene-2-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3- (3-methoxyphenyl) thiophene-2-sulfonamide, N- (4-bro Mo-3-methyl-5-isoxazolyl) -3- (3-methoxyphenyl) thiophen-2-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3 -(2-methoxyphenyl) thiophene-2-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-ethylphenyl) thiophene-2-sulfonamide , N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-propylphenyl) thiophene-2-sulfonamide, N- (4-bromo-3-methyl-5- Isoxazolyl) -3- (4-isopropylphenyl) thiophene-2-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-butylphenyl) thi Offen-2-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3- (2,4-dimethylphenyl) thi Phen-2-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-isobutylphenyl) thiophene-2-sulfonamide, N- (4-bromo -3-methyl-5-isoxazolyl) -3- (4-isopentylphenyl) thiophene-2-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3- (2-methyl-4-propylphenyl) thiophene-2-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-isobutyl-2-methylphenyl) thi Fen-2-sulfonamide and N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-isopentyl-2-methylphenyl) thiophene-2-sulfonamide in N- (4 -Bromo-3-methyl-5-isoxazolyl) -3-[(3,4-methylenedioxy) phenyl] thiophene-2-sulfonamide (International Patent Application Publication WO 96 See Example 125 of / 31492).
[748] N- (4-bromo-3-methyl-5-isoxazolyl) -2- [2-methyl-4,5- (methylenedioxy) phenethyl] thiophene-3-sulfonamide is N- (4 -Bromo-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) phenethyl] thiophene-3-sulfonamide (Example 22). N- (4-bromo-3-methyl-5-isoxazolyl) -2- [2-methyl-4,5- (methylenedioxy) cinnamyl] thiophene-3-sulfonamide is N- (4 -Bromo-3-methyl-5-isoxazolyl) -2-[(4-methyl) (cinnamil)] thiophen-3-sulfonamide in the same manner as in Example 23.
[749] N- (4-bromo-3-methyl-5-isoxazolyl) -2-{[3,4- (methylenedioxy) phenoxy] methyl} thiophene-3-sulfonamide, N- (4- Bromo-3-methyl-5-isoxazolyl) -2-[(2,4,6-trimethylphenoxy) methyl] thiophene-3-sulfonamide, N- (4-bromo-3-methyl- 5-isoxazolyl) -2-{[4,5- (methylenedioxy) -2-propylphenoxy] methyl} thiophene-3-sulfonamide is N- (4-bromo-3-methyl-5 -Isoxazolyl) -2-[(4-methylphenoxy) methyl] thiophene-3-sulfonamide (Example 25).
[750] Any such compound or any corresponding N- (4-halo-3-methyl-5-isoxazolyl), N- (4-halo-5-methyl-3-isoxazolyl) of any of the compounds described herein ), N- (3,4-dimethyl-5-isoxazolyl), N- (4-halo-5-methyl-3-isoxazolyl), N- (4-halo-3-methyl-5-isox Sazolyl), N- (4,5-dimethyl-3-isoxazolyl) derivatives may also be prepared and used as described herein. Pharmaceutically acceptable derivatives comprising salts, in particular sodium salts, are used in the formulation as described herein.
[751] Example 37
[752] Other compounds that may be prepared by the above methods or conventional modifications thereof include, but are not limited to:
[753] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,3,4-trimethoxy-6-methylphenylaminocarbonyl) thiophene-3-sulfonamide,
[754] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,3,4-trimethoxy-6-acetylphenylaminocarbonyl) thiophene-3-sulfonamide,
[755] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,3,4-trimethoxy-6-methoxycarbonylphenylaminocarbonyl) thiophen-3-sulfonamide,
[756] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,3,4-trimethoxy-6-carboxyphenylaminocarbonyl) thiophen-3-sulfonamide,
[757] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,3,4-trimethoxy-6-methanesulfonylphenylaminocarbonyl) thiophen-3-sulfonamide,
[758] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [2,3,4-trimethoxy-6- (cyanomethyl) phenylaminocarbonyl] thiophen-3-sulfonamide ,
[759] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [2,3,4-trimethoxy-6- (2-hydroxyethyl) phenylaminocarbonyl] thiophen-3- Sulfonamide,
[760] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-methoxy-6-methylphenylaminocarbonyl] thiophen-3-sulfonamide ,
[761] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-methoxy-6-acetylphenylaminocarbonyl] thiophen-3-sulfone amides,
[762] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-methoxy-6-methoxycarbonylphenylaminocarbonyl] thiophene- 3-sulfonamide,
[763] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-methoxy-6-carboxylphenylaminocarbonyl] thiophen-3-sulfone amides,
[764] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-methoxy-6-methanesulfonylphenylaminocarbonyl] thiophen-3 Sulfonamides,
[765] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-methoxy-6-cyanophenylaminocarbonyl] thiophen-3- Sulfonamide,
[766] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-methoxy-6-cyanomethylphenylaminocarbonyl] thiophen-3- Sulfonamide,
[767] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-methoxy-6- (2-hydroxyethyl) phenylaminocarbonyl] Thiophene-3-sulfonamide,
[768] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2,6-dimethylphenylaminocarbonyl] thiophene-3-sulfonamide,
[769] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-acetyl-2-methylphenylaminocarbonyl] thiophen-3-sulfonamide,
[770] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methoxycarbonyl-2-methylphenylaminocarbonyl] thiophen-3- Sulfonamide,
[771] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-carboxy-2-methylphenylaminocarbonyl] thiophen-3-sulfonamide,
[772] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methoxy-2-methylphenylaminocarbonyl] thiophen-3-sulfonamide ,
[773] -(4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methanesulfonyl-2-methylphenylaminocarbonyl] thiophen-3-sulfonamide ,
[774] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-cyano-2-methylphenylaminocarbonyl] thiophen-3-sulfonamide ,
[775] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6- (cyanomethyl) -2-methylphenylaminocarbonyl] thiophen-3 Sulfonamides,
[776] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6- (2-hydroxyethyl) -2-methylphenylaminocarbonyl] thiophene -3- sulfonamide,
[777] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-cyano-6-methylphenylaminocarbonyl] thiophen-3-sulfonamide ,
[778] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methoxy-2-cyanophenylaminocarbonyl] thiophen-3- Sulfonamide,
[779] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-acetyl-6-methylphenylaminocarbonyl] thiophene-3-sulfonamide,
[780] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methoxy-2-acetylphenylaminocarbonyl] thiophen-3-sulfone amides,
[781] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-cyano-2,4,6-trimethylphenylaminocarbonyl] thiophene-3-sulfonamide,
[782] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-carboxy-2-2,4,6-trimethylphenylaminocarbonyl] thiophene-3-sulfonamide,
[783] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-hydroxymethyl-2,4,6-trimethylphenylaminocarbonyl] thiophene-3-sulfonamide,
[784] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-methanesulfonyl-2,4,6-trimethylphenylaminocarbonyl] thiophene-3-sulfonamide,
[785] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-cyanomethyl-2,4,6-trimethylphenylaminocarbonyl] thiophene-3-sulfonamide,
[786] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3- (2-hydroxyethyl) -2,4,6-trimethylphenylaminocarbonyl] thiophen-3-sulfonamide ,
[787] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3- (carboxymethyl) -2,4,6-trimethylphenylaminocarbonyl] thiophen-3-sulfonamide,
[788] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (4-cyano-2,6-dimethylphenylaminocarbonyl) thiophen-3-sulfonamide,
[789] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (4-carboxyl-2,6-dimethylphenylaminocarbonyl) thiophene-3-sulfonamide,
[790] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [4- (hydroxymethyl) -2,6-dimethylphenylaminocarbonyl] thiophene-3-sulfonamide,
[791] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [4- (2-hydroxyethyl) -2,6-dimethylphenylaminocarbonyl] thiophene-3-sulfonamide,
[792] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [4- (cyanomethyl) -2,6-dimethylphenylaminocarbonyl] thiophene-3-sulfonamide,
[793] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [4- (carboxymethyl) -2,6-dimethylphenylaminocarbonyl] thiophene-3-sulfonamide,
[794] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (4-methanesulfonyl-2,6-dimethylphenylaminocarbonyl) thiophene-3-sulfonamide,
[795] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,3,4-trimethoxy-6-methylphenylacetyl) thiophene-3-sulfonamide,
[796] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,3,4-trimethoxy-6-acetylphenylacetyl) thiophene-3-sulfonamide,
[797] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,3,4-trimethoxy-6-methoxycarbonylphenylacetyl) thiophene-3-sulfonamide,
[798] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,3,4-trimethoxy-6-carboxyphenylacetyl) thiophene-3-sulfonamide,
[799] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2,3,4-trimethoxy-6-methanesulfonylphenylacetyl) thiophene-3-sulfonamide,
[800] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [2,3,4-trimethoxy-6- (cyanomethyl) phenylacetyl] thiophene-3-sulfonamide,
[801] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [2,3,4-trimethoxy-6- (2-hydroxyethyl) phenylacetyl] thiophene-3-sulfonamide ,
[802] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-methoxy-6-methylphenylacetyl] thiophene-3-sulfonamide,
[803] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-methoxy-6-acetylphenylacetyl] thiophene-3-sulfonamide,
[804] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-methoxy-6-methoxycarbonylphenylacetyl] thiophen-3- Sulfonamide,
[805] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-methoxy-6-carboxyphenylacetyl] thiophene-3-sulfonamide,
[806] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-methoxy-6-methanesulfonylphenylacetyl] thiophen-3-sulfone amides,
[807] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-methoxy-6- (cyano) phenylacetyl] thiophen-3- Sulfonamide,
[808] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-methoxy-6- (cyanomethylphenylacetyl] thiophen-3-sulfone amides,
[809] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-methoxy-6- (2-hydroxyethyl) phenylacetyl] thiophene -3- sulfonamide,
[810] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2,6-dimethylphenylacetyl] thiophene-3-sulfonamide,
[811] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-acetyl-2-methylphenylacetyl] thiophene-3-sulfonamide,
[812] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methoxycarbonyl-2-methylphenylacetyl] thiophen-3-sulfonamide ,
[813] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-carboxy-2--2-methylphenylacetyl] thiophene-3-sulfonamide,
[814] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methoxy-2-methylphenylacetyl] thiophene-3-sulfonamide,
[815] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methanesulfonyl-2-methylphenylacetyl] thiophene-3-sulfonamide,
[816] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-cyano-2-methylphenylacetyl] thiophene-3-sulfonamide,
[817] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6- (cyanomethyl) -2-methylphenylacetyl] thiophen-3-sulfone amides,
[818] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6- (2-hydroxyethyl) -2-methylphenylacetyl] thiophen-3 Sulfonamides,
[819] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-cyano-6-methylphenylacetyl] thiophene-3-sulfonamide,
[820] N- (4-Chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methoxy-2-cyanophenylacetyl] thiophene-3-sulfonamide ,
[821] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -2-acetyl-6-methylphenylacetyl] thiophene-3-sulfonamide,
[822] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) -6-methoxy-2-acetylphenylacetyl] thiophene-3-sulfonamide,
[823] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-cyano-2,4,6-trimethylphenylacetyl) thiophene-3-sulfonamide,
[824] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-carboxyl-2,4,6-trimethylphenylacetyl) thiophene-3-sulfonamide,
[825] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-hydroxymethyl-2,4,6-trimethylphenylacetyl) thiophene-3-sulfonamide,
[826] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3-methanesulfonyl-2,4,6-trimethylphenylacetyl) thiophene-3-sulfonamide,
[827] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3- (cyanomethyl) -2,4,6-trimethylphenylacetyl] thiophen-3-sulfonamide,
[828] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3- (2-hydroxyethyl) -2,4,6-trimethylphenylacetyl] thiophene-3-sulfonamide,
[829] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3- (carboxymethyl) -2,4,6-trimethylphenylacetyl] thiophene-3-sulfonamide,
[830] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (4-cyano-2,6-dimethylphenylacetyl) thiophene-3-sulfonamide,
[831] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (4-carboxyl-2,6-dimethylphenylacetyl) thiophene-3-sulfonamide,
[832] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (4-hydroxymethyl-2,6-dimethylphenylacetyl) thiophene-3-sulfonamide,
[833] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [4- (2-hydroxyethyl) -2,6- (dimethyl) phenylacetyl] thiophene-3-sulfonamide,
[834] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [4-cyanomethyl-2,6-dimethylphenylacetyl] thiophene-3-sulfonamide,
[835] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [4- (carboxymethyl) -2,6-dimethylphenylacetyl] thiophen-3-sulfonamide and
[836] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [4-methanesulfonyl-2,6-dimethylphenylacetyl] thiophene-3-sulfonamide. Pharmaceutically acceptable derivatives comprising salts, in particular sodium salts, are used for the formulations described herein.
[837] Example 38
[838] Heterocyclic rings, such as thienyl-, furyl- and pyrrole-sulfonamides, which generally have an activity at 10 μM or substantially lower IC ₅₀ concentrations for the ET _A or ET _B receptors, wherein Ar ² is of interest Other compounds, including, may be prepared by methods analogous to those set forth in the examples above, or have been prepared (see, eg, Tables 1A-1R). Such compounds include, but are not limited to: N- (4-bromo-3-methyl-5-isoxazolyl) -2-carboxyl-1-methylindole-3-sulfonamide, N- (4 -Chloro-3-methyl-5-isoxazolyl) -2-[(4-oxacyclohexyl) oxycarbonyl] thiophene-3-sulfonamide, 2- [3,4- (methylenedioxy) phenylacetyl ] Thiophene-3-sulfonamide, N- (4-chloro-3-methyl-5-isoxazolyl)-{2- [3,4- (methylenedioxy) phenyl] acetyl} thiophene-3-sulfone Amide oxime, N- (4-chloro-3-methyl-5-isoxazolyl) -2-phenylbenzo [b] thiophene sulfonamide, N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(4-tolyl) aminocarbonyl] -1-methylindole-3-sulfonamide, N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(4-methoxy Phenoxy) carbonyl] thiophene-3-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -1- [3,4- (methylenedioxy) benzyl] indole-2 -Sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(4-methylphenoxy) carbonyl] thiophene-3-sulfonamide, N- (4- Chloro-3-methyl-5-isoxazolyl) -2-[(4-methoxyphenyl) acetyl] thiophene-3-sulfonamide, N- (4-chloro-3-methyl-5-isoxazolyl) -6-methoxy-2- [3,4- (methylenedioxy) benzyl] benzo [b] thiophene-3-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3-[(4-methoxyphenoxy) methyl] thiophene-2-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(4-methylphenoxy ) Methyl] thiophen-3-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-methyl-trans-styryl) thiophen-2-sulfonamide, N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-methylphenethyl) thiophene-2-sulfonamide, N- (4-bromo-3-methyl-5- Isoxazolyl) -2-[(4-methylphenyl) acetyl] thiophen-3-sulfonamide, N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(3-methoxyphenyl ) Acetyl] thiophen-3-sulfonamide, N- (4-chloro-3-methyl-5-isoxazolyl) -2- {1-hydroxy-1- [3,4- (methylenedioxy) benzyl ] Ethyl} thiophene-3-sulfonamide, N- (4-bromo-3-methyl-5-iso Thiazolyl) -3- (4-methyl-phenethyl) (4-tolyl) thiophene-2-sulfonamide,
[839] N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-methylbenzyl) -5- (4-tolyl) thiophene-2-sulfonamide, N- (4-bromo -3-methyl-5-isoxazolyl) -3- (4-methyl-trans-styryl) -5- (4-tolyl) thiophene-2-sulfonamide, N- (4-chloro-3-methyl -5-isoxazolyl) -2- [β, β- (ethylenedioxy) 3,4- (methylenedioxy) phenethyl] thiophen-3-sulfonamide, N- (4-chloro-3-methyl -5-isoxazolyl) -2- [β- (dimethylamino) -3,4- (methylenedioxy) phenethyl] thiophene-3-sulfonamide, N- (4-chloro-3-methyl-5 Isoxazolyl) -2- {α-hydroxy- [3,4- (methylenedioxy) phenyl] acetyl} thiophene-3-sulfonamide; N- (4-chloro-5-methyl-3-isoxazolyl) -2- [3,4- (methylenedioxy) benzyl] benzo [b] thiophene-3-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -3-styrylthiophene-2-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -2-styrylthiophen-3-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -2- (benzoylamino) thiophen-3-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -2-[(phenyl) methylaminocarbonyl] thiophene-3-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -5- (phenylthio) furan-2-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -5- (hydroxymethyl) furan-2-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -5- (carmethoxy) furan-2-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -2,5-dimethylfuran-3-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -2- (diisopropylaminocarbonyl) thiophene-3-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -2- (diethylaminocarbonyl) thiophen-3-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -5-styrylfuran-2-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -5-styrylthiophene-2-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxybenzyl) -5- (dimethylamino) benzo [b] thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) benzyl] -7-methoxybenzo [b] thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) benzyl] -7-phenoxybenzo [b] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) benzyl] -5-methoxybenzo [b] thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) benzyl] -5-isobutylaminobenzo [b] thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) benzyl] -5-benzylaminobenzo [b] thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) phenoxy] benzo [b] thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) phenoxy] -5-dimethylaminobenzo [b] thiophene-3-sulfonamide;
[840] N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) phenyl] acetyl-5-dimethylaminobenzo [b] thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) benzylcarbonyl] -N-methylindole-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) phenoxycarbonyl] indole-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) phenoxycarbonyl] -N-methylindole-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) phenoxycarbonyl] indole-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) benzyl] -N-methylindole-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) benzyl] indole-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) benzyloxycarbonyl] -7- (N, N-dimethylamino) benzo [b] Thiophene-3-sulfonamide; N- (4-Chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) benzyl] -7- (N, N-dimethylamino) benzo [b] thiophene- 3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) benzoyl] -7- (N, N-dimethylamino) benzo [b] thiophene- 3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -7- (N, N-dimethylamino) benzo [b] thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -7- (methoxycarbonyl) benzo [b] thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) benzyl] -7- (methoxy) benzo [b] thiophen-3-sulfonamide ; N- (4-chloro-3-methyl-5-isoxazolyl) -7- (methoxy) benzo [b] thiophene-3-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -2- (4-methylphenethyl) thiophen-3-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -2- (trans-4-methylcinnamil) thiophene-3-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -3- (4-methylphenethyl) thiophene-2-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -3- (3-methylphenethyl) thiophene-2-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -3- (2-methylphenethyl) thiophene-2-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -3- (trans-4-methylcinnamil) thiophene-2-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -3- (trans-3-methylcinnamil) thiophene-2-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -3- (trans-2-methylcinnamil) thiophene-2-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -3-[(4-methylphenoxy) methyl] thiophene-2-sulfonamide; N- (4-bromo-3-methyl-5-isoxazolyl) -3- [3,4- (methylenedioxy) phenethyl] thiophene-2-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(3,4-dimethoxyphenyl) acetyl] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(3,5-dimethoxyphenyl) acetyl] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(3,4,5-trimethoxyphenyl) acetyl] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) benzylsulfonyl] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) benzylsulfinyl] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) benzylsulphenyl] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- {1- (dimethylamino) -2- [3,4- (methylenedioxy) phenyl] ethyl} thiophene-3-sulfone amides; N- (4-chloro-3-methyl-5-isoxazolyl) -2- {1- (methylamino) -2- [3,4- (methylenedioxy) phenyl] ethyl} thiophen-3-sulfone amides; N- (4-chloro-3-methyl-5-isoxazolyl) -2- {1- (methoxyxylimino) -2- [3,4- (methylenedioxy) phenyl] ethyl} thiophen-3 Sulfonamides; N- (4-chloro-3-methyl-5-isoxazolyl) -2- {1- (carboxyl) -2- [3,4- (methylenedioxy) phenyl] ethyl} thiophen-3-sulfonamide ; N- (4-chloro-3-methyl-5-isoxazolyl) -2- {2- (carboxyl) -1- [3,4- (methylenedioxy) benzyl] vinyl} thiophen-3-sulfonamide ; N- (4-chloro-3-methyl-5-isoxazolyl) -2- {3- [3,4- (methylenedioxy) phenyl] -1,2,4-oxadiazol-5-yl} Thiophene-3-sulfonamide; And N- (4-chloro-3-methyl-5-isoxazolyl) -2- {3- [3,4- (methylenedioxy) benzyl] -1,2,4-oxadiazol-5-yl } Thiophene-3-sulfonamide.
[841] Additional compounds include, but are not limited to: N- (4-chloro-3-methyl-5-isoxazolyl) -2-{[2- (methanesulfonyl) -4,5- (methylene Dioxy) phenyl] aminocarbonyl} thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-{[3,4- (methylenedioxy) -6-carboxylphenyl] aminocarbonyl} thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-{[4,5- (methylenedioxy) -2- (methoxycarbonyl)] phenyl} aminocarbonyl} thiophene- 3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-{[2-cyano-4,5- (methylenedioxy) phenyl] aminocarbonyl} thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-{[4,5- (methylenedioxy) -2- (hydroxymethyl) phenyl] aminocarbonyl} thiophen-3- Sulfonamides; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(2-acetyl-4-methylphenyl] aminocarbonyl] thiophene-3-sulfonamide; N- (4-chloro-3 -Methyl-5-isoxazolyl) -2-{[(2- (methanesulfonyl) -4-methylphenyl] aminocarbonyl} thiophene-3-sulfonamide; N- (4-chloro-3-methyl- 5-isoxazolyl) -2-[(2-carboxy-4-methylphenyl) aminocarbonyl] thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2 -[(2-methoxycarbonyl-4-methylphenyl) aminocarbonyl] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(2- Cyano-4-methylphenyl) aminocarbonyl] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-{[2- (hydroxymethyl) -4 -Methylphenyl] aminocarbonyl} thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(3,4-dimethoxy-6-acetylphenyl) amino Carbonyl] thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-{[2- (methanesulfonyl) -4,5-dimethoxyphenyl] amino Cabo } Thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(4,5-dimethoxy-2-carboxyphenyl) aminocarbonyl] thiophene- 3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(4,5-dimethoxy-2-methoxycarboxy) phenyl) aminocarbonyl] thiophen-3 -Sulfonamide N- (4-chloro-3-methyl-5-isoxazolyl) -2- [2-cyano (4,5-dimethoxyphenyl) aminocarbonyl] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- (4,5-dimethoxy-2-hydroxymethyl) phenylaminocarbonylthiophen-3-sulfonamide; N- (4- Chloro-3-methyl-5-isoxazolyl) -2-{[2-acetyl-4,5- (methylenedioxy) phenyl] acetyl} thiophene-3-sulfonamide; N- (4-chloro-3 -Methyl-5-isoxazolyl) -2-{[2- (methanesulfonyl) -4,5- (methylenedioxy) phenyl] acetyl} thiophene-3-sulfonamide; N- (4-chloro- 3-methyl-5-isoxazolyl) -2-{[carboxyl-4,5- (methylenedioxy) -2-phenyl] acetyl} thiophene-3-sulfonamide; N- (4-chloro-3- Methyl-5-speed Thiazolyl) -2 - {[4,5- (methylenedioxy) -2-methoxy-carbonyl-phenyl] acetyl} thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- {2-cyano [4,5- (methylenedioxy) phenyl] acetyl} thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- {2-hydroxymethyl [4,5- (methylenedioxy) phenyl] acetyl} thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(2,4-dimethoxy) phenyl] aminocarbonyl} thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(4-methoxy-2-methylphenyl) aminocarbonyl] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(2,3-dimethylphenyl) aminocarbonyl] thiophene-3-sulfonamide;
[842] N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(2,4-dimethylphenyl) aminocarbonyl] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(2,5-dimethylphenyl) aminocarbonyl] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(2,6-dimethylphenyl) aminocarbonyl] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(3,4-dimethylphenyl) aminocarbonyl] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(2,5-dimethylphenyl) aminocarbonyl] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2- (3,5-dimethyl) phenylaminocarbonylthiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(2-methoxy-6-methylphenyl) aminocarbonyl] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(2,4,6-trimethylphenyl) aminocarbonyl] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(4-methoxy-2-methylphenyl) aminocarbonyl] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(2-ethyl-4-methoxyphenyl) aminocarbonyl] thiophen-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(2-isopropyl-4-methoxyphenyl) aminocarbonyl] thiophen-3-sulfonamide; N- (4- Chloro-3-methyl-5-isoxazolyl) -2-[(2-propyl-4-methoxyphenyl) aminocarbonyl] thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxazolyl) -2-[(4-methoxy-2-biphenylaminocarbonyl] thiophene-3-sulfonamide; N- (4-chloro- 3-methyl-5-isoxazolyl) -2-{[3,4- (methylenedioxy) -6-methylphenyl] acetyl} thiophene-3-sulfonamide; N- (4-chloro-3-methyl- 5-isoxazolyl) -2-{[3,4- (methylenedioxy) -6-ethylphenyl] acetyl} thiophene-3-sulfonamide; N- (4-chloro-3-methyl-5-isoxe Sazolyl) -2-{[3,4- (methylenedioxy) -6-methoxyphenyl) acetyl} thiophene-3-sulfonamide.
[843] Pharmaceutically acceptable derivatives comprising salts, in particular sodium salts, are used in the formulations described herein.
[844] Example 39
[845] N- (2-acetyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thiophencarboxamide
[846] A. 2'-amino-3 ', 5'-dimethylacetophenone
[847] To a solution of BCl ₃ in dichloromethane (1.0M, 25ml) is slowly added 2,4-dimethylaniline (3.03g, 25mmol) in 1,2-dichloroethane (25ml) at 0 ° C. Next, acetonitrile (25 ml) is added dropwise at 0 ° C. The mixture is heated in a bath at 100 ° C. slowly and steadily with nitrogen for 2 days to remove low boiling dichloromethane. The reaction is cooled to 0 ° C., quenched with 2N HCl (˜25 ml) and the mixture is heated to 80 ° C. until a homogeneous solution is produced (˜20 min). It is cooled to room temperature and the two layers are separated. No gas evolution is observed and the aqueous layer is basified with sodium bicarbonate until many precipitates are formed. The mixture is extracted with chloroform (-30 ml), the organic layers are combined and concentrated. The residue is dissolved in ethyl acetate (50 ml) and washed with 1N NaOH (40 ml). The organic layer is then dried (MgSO ₄ ), the solid is filtered off and the filtrate is concentrated. The oily residue is dissolved in ethyl ether (˜5 ml) and left at room temperature for 24 hours. The resulting yellow precipitate is filtered to give 2'-amino-3 ', 5'-dimethylacetophenone (1.3 g, 30% yield).
[848] B. N- (2-acetyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thiophencarboxamide
[849] N- (4-chloro-3-methyl-5-isoxazolyl) -N- in a solution of 2'-amino-3 ', 5'-dimethylacetophenone (1.9 g, 11.66 mmol) in dichloromethane (20 ml) Methoxymethyl-3-sulfamoyl-2-thiophencarbonylchloride (Example 51) (1 g, 2.86 mmol) is added at room temperature. The mixture is stirred for 10 hours, during which time a large amount of yellow precipitate is produced. The reaction is then concentrated and the residue is diluted with ethyl acetate (50 ml) and washed with 1N HCl (50 ml). The organic layer is concentrated, the residue is dissolved in methanol (30 ml) and then concentrated HCl (15 ml) is added. The mixture is heated at reflux for 2 h, then cooled to rt, diluted with ethyl acetate (200 ml) and washed with water (2 × 200 ml). The organic layer is separated, dried (MgSO ₄ ), the solid is filtered and the filtrate is concentrated. The residue is purified by reverse phase HPLC to give N- (2-acetyl-4,6-dimethylphenyl) -3-((( 4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thiophencarboxamide (580 mg, 43% yield) is obtained.
[850] Example 40
[851] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-propionylphenyl) -2-thiophencarboxamide
[852] A. 2'-amino-3 ', 5'-dimethylpropiophenone
[853] 2'-amino-3 ', 5'-dimethylpropy in the same manner as 2'-amino-3', 5'-dimethylacetophenone (Example 39), except that propionitrile is used instead of acetonitrile. Prepare fenones.
[854] B. 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-propionylphenyl) -2-thiophencarboxamide
[855] N- (2-acetyl-4,6-dimethyl except that 2'-amino-3 ', 5'-dimethylpropiophenone is used instead of 2'-amino-3', 5'-dimethylacetophenone Phenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thiophencarboxamide (Example 39) in the same manner as in 3-(((4 -Chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2,4-dimethyl-6-propionylphenyl) -2-thiophenecarboxamide is prepared.
[856] Example 41
[857] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2-isobutyryl-4,6-dimethylphenyl) -2-thiophencarboxamide
[858] A. 2'-Amino-3 ', 5'-dimethyl-2-methylpropiophenone
[859] 2'-amino-3 ', 5'-dimethyl in the same manner as 2'-amino-3', 5'-dimethylacetophenone (Example 39), except that isobutyronitrile is used instead of acetonitrile. 2-Methylpropiophenone is prepared.
[860] B. 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2-isobutyryl-4,6-dimethylphenyl) -2-thiophencarbox amides
[861] N- (2-acetyl-4, except that 2'-amino-3 ', 5'-dimethyl-2-methylpropiophenone is used instead of 2'-amino-3', 5'-dimethylacetophenone. , 6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thiophenecarboxamide (Example 39) in the same manner as in 3- Prepare (((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2-isobutyryl-4,6-dimethylphenyl) -2-thiophencarboxamide .
[862] Example 42
[863] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (cyclohexylcarbonyl) -4,6-dimethylphenyl) -2-thiophenka Voxamide
[864] A. Cyclohexyl 2-amino-3,5-dimethylphenyl ketone
[865] Cyclohexyl 2-amino-3,5-dimethylphenyl in the same manner as 2'-amino-3 ', 5'-dimethylacetophenone (Example 39), except that cyclohexyl cyanide is used instead of acetonitrile. Prepare ketones.
[866] B. 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (cyclohexylcarbonyl) -4,6-dimethylphenyl) -2-ti Offencarboxamide
[867] N- (2-acetyl-4,6-dimethylphenyl) except that cyclohexyl 2-amino-3,5-dimethylphenyl ketone is used instead of 2'-amino-3 ', 5'-dimethylacetophenone. 3-(((4-chloro in the same manner as in 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thiophencarboxamide (Example 39) 3-Methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (cyclohexylcarbonyl) -4,6-dimethylphenyl) -2-thiophenecarboxamide is prepared.
[868] Example 43
[869] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (cyclopropylcarbonyl) -4,6-dimethylphenyl) -2-thiophenka Voxamide
[870] A. Cyclopropyl 2-amino-3,5-dimethylphenyl ketone
[871] Cyclopropyl 2-amino-3,5-dimethylphenyl in the same manner as 2'-amino-3 ', 5'-dimethylacetophenone (Example 39), except that cyclopropyl cyanide is used instead of acetonitrile. Prepare ketones.
[872] B. 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (cyclopropylcarbonyl) -4,6-dimethylphenyl) -2-ti Offencarboxamide
[873] N- (2-acetyl-4,6-dimethylphenyl) except that cyclopropyl 2-amino-3,5-dimethylphenyl ketone is used instead of 2'-amino-3 ', 5'-dimethylacetophenone. 3-(((4-chloro in the same manner as in 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thiophencarboxamide (Example 39) 3-Methyl-5-isoxazolyl) amino) sulfonyl) -N- (2- (cyclopropylcarbonyl) -4,6-dimethylphenyl) -2-thiophenecarboxamide is prepared.
[874] Example 44
[875] N- (2-benzoyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thiophencarboxamide
[876] A. 2-amino-3,5-dimethylphenyl phenyl ketone
[877] 2-amino-3,5-dimethylphenyl phenyl ketone was prepared in the same manner as 2'-amino-3 ', 5'-dimethylacetophenone (Example 39), except that benzonitrile was used instead of acetonitrile. do.
[878] B. N- (2-benzoyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thiophencarboxamide
[879] N- (2-acetyl-4,6-dimethylphenyl)-except that 2-amino-3,5-dimethylphenyl phenyl ketone is used instead of 2'-amino-3 ', 5'-dimethylacetophenone. N- (2-benzoyl-4, in the same manner as 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thiophenecarboxamide (Example 39) 6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thiophenecarboxamide is prepared.
[880] Example 45
[881] N- (2-acetyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -5-methyl-2-thiophencarbox amides
[882] A. N- (4-Chloro-3-methyl-5-isoxazolyl) -3-sulfamoyl-5-methyl-2-thiophencarboxylic acid
[883] NBuLi (2.5 in hexane) to a solution of N- (4-chloro-3-methyl-5-isoxazolyl) -3-sulfamoyl-2-thiophencarboxylic acid (6 g, 18.60 mmol) in anhydrous tetrahydrofuran (240 ml) M, 30 ml, 74.4 mmol) is added at -78 ° C in a nitrogen atmosphere. The mixture is stirred at this temperature for 2 hours and then iodomethane (6.6 g, 74.4 mmol) is added. The mixture is poured onto crushed ice and warmed to room temperature. Acidify to pH ˜1 with concentrated HCl, then extract the mixture with ethyl acetate (2 × 200 ml). Combine the organic layers, dry (MgSO ₄ ), filter the solids and concentrate the filtrate to N- (4-chloro-3-methyl-5-isoxazolyl) -3-sulfamoyl-5-methyl-2- Thiophencarboxylic acid and starting material are obtained in a ratio of about 2: 1 (total weight 8.5 g).
[884] B. N- (4-Chloro-3-methyl-5-isoxazolyl) -3-sulfamoyl-5-methyl-2-thiophencarboxylic acid
[885] To the solution of the resulting mixture (8.5 g) in Example 45A in THF (150 ml) was added sequentially diisopropylethylamine (9.62 g, 74.4 mmol) and bromomethyl methyl ether (90%, 7.75 g, 55.80 mmol). do. The mixture is stirred for 10 hours, then morpholine is added to remove excess bromomethyl methyl ether. The reaction is stirred for an additional 30 minutes, then diluted with ethyl acetate (150 ml) and washed with 1N HCl (200 ml). The organic layer is dried (MgSO ₄ ), the solid is filtered off and the filtrate is concentrated. The residue was chromatographed (10% ethyl acetate in hexane) to give methoxymethyl N- (4-chloro-3-methyl-5-isoxazolyl) -3-sulfamoyl-5-methyl-2-thiophencarboxylate. To obtain. Hydrolysis of the carboxylate with 1N NaOH affords the corresponding carboxylic acid (3.5 g).
[886] C. N- (4-Chloro-3-methyl-5-isoxazolyl) -N-methoxymethyl-3-sulfamoyl-5-methyl-2-thiophencarboxylic acid chloride
[887] N- (4-chloro-3-methyl-5-isox instead of N- (4-chloro-3-methyl-5-isoxazolyl) -N-methoxymethyl-3-sulfamoyl-2-thiophencarboxylic acid N- (4-chloro-3-methyl-5-isoxazolyl) -N, except for using sazolyl) -N-methoxymethyl-3-sulfamoyl-5-methyl-2-thiophencarboxylic acid N- (4-chloro-3-methyl-5-isoxazolyl) -N-methoxymethyl- in the same manner as the methoxymethyl-3-sulfamoyl-2-thiophencarbonyl chloride (Example 51) Prepare 3-Sulfamoyl-5-methyl-2-thiophencarboxylic acid chloride.
[888] D. N- (2-acetyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -5-methyl-2-thiophene Carboxamide
[889] N- (4-chloro-3-methyl-5-isoxazolyl) -N- (4-chloro-3-methyl-5-instead of N-methoxymethyl-3-sulfamoyl-2-thiophencarboxylic acid chloride N- (2-acetyl-4,6-dimethylphenyl) -3- except using isoxazolyl) -N-methoxymethyl-3-sulfamoyl-5-methyl-2-thiophencarboxylic acid chloride N- (2-acetyl-4,6- in the same manner as (((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thiophenecarboxamide (Example 39) Dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -5-methyl-2-thiophencarboxamide is prepared.
[890] Example 46
[891] 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2-hydroxyethaneimidoyl) -4,6-dimethylphenyl) -2-thiophenka Voxamide
[892] N- (2-acetyl-4,6-dimethylphenyl) -3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) in 2N NaOH (40 ml) and methanol (4 ml) To a solution of -2-thiophenecarboxamide (Example 39) (50 mg, 0.11 mmol) is added hydroxylamine hydrochloride (4 g, 57.6 mmol). The mixture is heated at 60 ° C. for 3 hours, then cooled to 0 ° C. and acidified to pH 1-2 with concentrated HCl. The resulting white precipitate was filtered, diluted with dilute acid and lyophilized to afford 3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -N- (2-hydroxyethane Imidoyl) -4,6-dimethylphenyl) -2-thiophenecarboxamide (45 mg, 87%) is obtained.
[893] Example 47
[894] 3-(((3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thienyl) carbonyl) amino) -2,4,6-trimethylphenyl methyl Carbonate
[895] N ^2- (4-chloro-3-methyl-5-isoxazolyl) -N- (3-hydroxy-2,4,6-trimethylphenyl) -3-sulfamoyl-2-thiophenca in anhydrous DMF To a solution of voxamide (Example 52) (238 mg, 0.524 mmol) potassium tert-butoxide (177 mg, 1.57 mmol) is added at 0 ° C. The mixture is stirred for 30 minutes at ionicity and then methyl chloroform (99.2 mg, 1.05 mmol) is added. The reaction was poured into frozen and diluted acid, the resulting precipitate was collected and purified by HPLC to obtain 3-(((3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl)- 2-thienyl) carbonyl) amino) -2,4,6-trimethylphenyl methyl carbonate (186 mg, 70%) is obtained.
[896] Example 48
[897] 3-(((3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl) -2-thienyl) carbonyl) amino) -2,4,6-trimethylphenyl carba Mate
[898] N ^2- (4-chloro-3-methyl-5-isoxazolyl) -N- (3-hydroxy-2,4,6-trimethylphenyl) -3-sulfamoyl-2-thiophenca in anhydrous DMF To a solution of boxamide (Example 52) (500 mg, 1.05 mmol) potassium tert-butoxide (295 mg, 2.61 mmol) was added at 0 ° C. The mixture is stirred at this temperature for 10 minutes and then p-nitrophenyl chloroformate (317 mg, 1.57 mmol) is added. After stirring for about 1 minute, the mixture is treated with ammonium hydroxide (8 ml) and stirring is continued for 2 hours at room temperature. The reaction was poured into frozen and diluted acid, the resulting precipitate was collected and purified by HPLC to obtain 3-(((3-(((4-chloro-3-methyl-5-isoxazolyl) amino) sulfonyl)- 2-thienyl) carbonyl) amino) -2,4,6-trimethylphenyl carbamate (213 mg, 42%) is obtained.
[899] Example 49
[900] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2- (3-methoxy-2,4,6-trimethylphenyl) acetyl) -3-thiophenesulfonamide
[901] A. N- (4-chloro-3-methyl-5-isoxazolyl) -3-sulfamoyl-2-thiophencarbonitrile
[902] A solution of N- (4-chloro-3-methyl-5-isoxazolyl) -3-sulfamoyl-2-thiophencarboxamide (5 g, 15.6 mmol) in POCl ₃ (50 ml) at 60 ° C. for 3 hours Heat during. The reaction is cooled to room temperature, poured into crushed ice (˜250 g), shaken the ice covered mixture and stirred until the ice melts (˜2 hours). The mixture is extracted with ethyl acetate, the organic layer is dried (MgSO ₄ ), the solid is filtered, the filtrate is concentrated and dried under vacuum to give N- (4-chloro-3-methyl-5-isoxazolyl)- 3-Sulfamoyl-2-thiophencarbonitrile (4.8 g, ˜100%) is obtained.
[903] B. 3-methoxy-2,4,6-trimethylbenzyl chloride
[904] 5-chloromethyl-6-methylbenzo [d] [1 except for using 1-methoxy-2,4,6-trimethylbenzene in place of 5-methylbenzo [d] [1,3] dioxol , 3] 3-methoxy-2,4,6-trimethylbenzyl chloride was prepared in the same manner as in the dioxol (Example 7).
[905] C. N- (4-Chloro-3-methyl-5-isoxazolyl) -2- (2- (3-methoxy-2,4,6-trimethylphenyl) acetyl) -3-thiophenesulfonamide
[906] N- (4-chloro-3-methyl instead of N ² -methoxy-N ² -methyl-3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophenecarboxamide 4-Chloro-3-methyl-5- (2- (2- (6- N- (4-chloro-3-methyl- in the same manner as for methylbenzo [d] [1,3] dioxol-5-yl) acetyl) -3-thienylsulfonamido) isoxazole (Example 7) 5-isoxazolyl) amino) sulfonyl) -2- (2- (3-methoxy-2,4,6-trimethylphenyl) acetyl) -3-thiophenesulfonamide is prepared.
[907] Example 50
[908] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2- (3-hydroxy-2,4,6-trimethylphenyl) acetyl) -3-thiophenesulfonamide
[909] N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2- (3-methoxy-2,4,6-trimethylphenyl) acetyl) -3-t in dichloromethane (20 ml) To a solution of openesulfonamide (Example 49) (50 mg, 0.107 mmol) is added BBr ₃ (1M in dichloromethane, 3 ml, 3.0 mmol) at 0 ° C. The resulting mixture is stirred at 0 ° C. for 1 hour, at room temperature for 8 hours and then poured onto crushed ice (˜100 g). The aqueous mixture is stirred until all the ice is dissolved and extracted with dichloromethane (2 x 100 ml). The organic layers were combined, concentrated and the residue was purified by HPLC to give N- (4-chloro-3-methyl-5-isoxazolyl) -2- (2- (3-hydroxy-2,4,6-tri Methylphenyl) acetyl) -3-thiophenesulfonamide (47 mg, 85%) is obtained.
[910] Example 51
[911] N- (4-chloro-3-methyl-5-isoxazolyl) -N-methoxymethyl-3-sulfamoyl-2-thiophencarbonyl chloride
[912] A. 5-amino-4-chloro-3-methylisoxazole
[913] To a solution of 5-amino-3-methylisoxazole (9.8 g, 100 mmol) in methylene chloride (200 ml) is added N-chlorosuccinimide (14.7 g, 110 mmol) at 0 ° C. over 20 minutes. The reaction mixture is stirred at room temperature for 2 hours. The reaction mixture is concentrated as work up and partitioned between 1N NaOH (150 ml) / ethyl acetate (400 ml). The organic layer is washed with 1N NaOH, water, brine, dried over MgSO ₄ and concentrated to give a brown solid. As purification, the product is reprecipitated from chloroform / hexanes and then recrystallized from ethyl acetate / hexanes to give 5-amino-4-chloro-3-methylisoxazole as a brown solid (5.5 g, 41%).
[914] B. 2-Carbomethoxy-3- [N- (4-chloro-3-methylisoxazol-5-yl)] thiophenesulfonamide
[915] To a slurry of 60% mineral oil suspension of NaH (8.5 g, 0.21 mol) in THF (100 ml) was added a solution of 5-amino-4-chloro-3-methylisoxazole (12.4 g, 92.4 mmol) in THF (65 ml). Add at -20 ° C. for 20 minutes in nitrogen atmosphere. After stirring for 10 minutes, a solution of 2-carbomethoxy-3-thiophenesulfonyl chloride (22.2 g, 92.4 mmol) in THF (65 ml) is added at -20 ° C for 15 minutes. The reaction mixture is stirred for 10 minutes and then quenched with H ₂ O (5 ml) at the same temperature. As a workup the reaction mixture is poured into 4N HCl and the product is extracted with ethyl acetate. The combined organic layers are washed with water and the compound is extracted with semi-saturated NaHCO ₃ . The combined basic solution is decolorized with activated carbon, cooled to 0 ° C. and acidified with 4N HCl. The product was isolated by filtration, washed with water and dried to give 2-carbomethoxy-3- [N- (4-chloro-3-methylisoxazol-5-yl)] thiophenesulfonamide as a white powder (23.4 g, 75%).
[916] C. 2-Carbomethoxy-3- [N- (4-chloro-3-methylisoxazol-5-yl) -N-methoxymethyl] thiophenesulfonamide
[917] Diisopropyl in a solution of 2-carbomethoxy-3- [N- (4-chloro-3-methylisoxazol-5-yl)] thiophenesulfonamide (3.3 g, 10.0 mmol) in THF (50 mL) Ethylamine (1.9 g, 15.0 mmol) is added at 0 ° C., followed by bromomethyl methyl ether (1.5 g, 12.0 mmol). The reaction mixture is stirred overnight at room temperature The reaction mixture is concentrated as a workup and partitioned between water and ethyl acetate. The organic layer was washed with water, brine, dried over MgSO ₄ and concentrated to 2-carbomethoxy-3- [N- (4-chloro-3-methyl-methylisoxazol-5-yl) -N- Methoxymethyl] thiophenesulfonamide is obtained as green oil (3.5 g, 90%).
[918] D. 2-carboxy-3- [N- (4-chloro-3-methylisoxazol-5-yl) -N-methoxymethyl] thiophenesulfonamide
[919] 2-carbomethoxy-3- [N- (4-chloro-3-methylisoxazol-5-yl) -N-methoxymethyl] thiophenesulfone in a mixture of THF (30 ml) and 1N NaOH (30 ml) Amide (3.0 g, 7.8 mmol) is stirred for 3 hours at room temperature. The reaction mixture is diluted with water (20 ml) and extracted with ethyl acetate (5 ml). The water solution is acidified with 1N HCl and extracted with ethyl acetate. The organic layer was washed with water, brine, dried over MgSO ₄ and concentrated to 2-carboxy-3- [N- (4-chloro-3-methylisoxazol-5-yl) -N-methoxymethyl] Thiophensulfonamide is obtained as an oil (quantitative yield).
[920] E. N- (4-Chloro-3-methyl-5-isoxazolyl) -N-methoxymethyl-3-sulfamoyl-2-thiophencarbonyl chloride
[921] 2-carboxy-3- [N- (4-chloro-3-methylisoxazol-5-yl) -N-methoxymethyl] thiophensulfonamide (1.5 in a mixture of THF (10 ml) and chloroform (5 ml) g, 4.1 mmol) is added pyridine (1 drop) at 0 ° C., followed by 2M solution of oxalyl chloride (4.5 ml, 9.0 mmol). The reaction mixture is stirred overnight at room temperature. As a workup, the reaction mixture is concentrated under reduced pressure to remove all volatiles. The desired product is obtained as a sticky oil, which solidifies upon standing.
[922] Example 52
[923] N ^2- (4-chloro-3-methyl-5-isoxazolyl) -N- (3-hydroxy-2,4,6-trimethylphenyl) -3-sulfamoyl-2-thiophencarboxamide
[924] A. 3-acetoxy-2,4,6-trimethylaniline
[925] To a solution of 2,4,6-trimethylphenol (10 g, 73.5 mmol) and triethylamine (11.1 g, 110.3 mmol) in ethyl acetate (200 ml) was added acetyl chloride (7.5 g, 95.6 mmol) at 0 ° C. The mixture is stirred overnight. The reaction is quenched with water and the organic layer is washed with 1N HCl. The organic layer is dried as usual and concentrated. The residue is nitrified with 70% HNO ₃ and concentrated H ₂ SO ₄ at room temperature. The brown reaction mixture is stirred for 1 hour and poured into ice-water. The product is extracted with ethyl acetate and the extract is washed with water, dried over MgSO ₄ and concentrated to give the desired nitro compound. This compound is reduced by adding ammonium chloride and zinc powder in methanol. The exothermic reactant is stirred vigorously until it is returned to room temperature. As a workup the crude mixture is filtered and the cake is washed with methanol. The methanolic solution is concentrated and the residue is partitioned between ethyl acetate and 1N NaOH. The organic layer is dried over MgSO ₄ and concentrated to afford 3-acetoxy-2,4,6-trimethylaniline.
[926] B. N ^2- (4-Chloro-3-methyl-5-isoxazolyl) -N- (3-hydroxy-2,4,6-trimethylphenyl) -3-sulfamoyl-2-thiophencarbox amides
[927] N ^2- (4-chloro-3-methyl-5-isoxazolyl) -N- (3-hydroxy-2 by reacting the amine (Example 52A) with the product of Example 51 at 0 ° C in THF. Prepare 4,6-trimethylphenyl) -3-sulfamoyl-2-thiophencarboxamide. The reaction mixture is warmed to room temperature and stirred for 2 hours. As a workup the reaction mixture is poured into 0.05N HCl and the product is extracted with ethyl acetate. The organic layer is washed with 0.05N HCl, water, semi-saturated NaHCO ₃ , water, brine, dried over MgSO ₄ and concentrated. Purified by column chromatography (silica, 40% ethyl acetate / hexane) to give N ^2- (4-chloro-3-methyl-5-isoxazolyl) -N- (3-hydroxy-2,4,6-tri Methylphenyl) -N-methoxymethyl-3-sulfamoyl-2-thiophencarboxamide is obtained. A solution of this carboxamide in THF and concentrated HCl is stirred at 65-72 ° C. for 3.5 hours. As a workup the reaction mixture is cooled and poured into water. The product is dissolved in ethyl acetate. The extract is washed with water, brine saturated NaHCO ₃ , brine, dried over MgSO ₄ and concentrated to give an oil. During the deprotection of the MOM group, the acetoxy group is hydrolyzed to the corresponding hydroxyl. N ^2- (4-chloro-3-methyl-5-isoxazolyl) -N- (3-hydroxy-2,4,6-trimethylphenyl) -3-sulfamoyl-2-thiophencarboxamide Obtained as a solid (melting point 75 to 78 ° C., 54%).
[928] Example 53
[929] Assays Identifying Compounds Showing Endothelin Antagonist and / or Agonist Activity
[930] Compounds that are potent endothelin antagonists are identified by testing their ability to compete with ¹²⁵ I-labeled ET-1 for binding to human ET _A receptor or ET _B receptor present on isolated cell membranes. The efficacy of test compounds as antagonists or agonists of endothelin's biological tissue response can also be assessed by measuring the effect on endothelin-induced contraction of the thoracic aortic ring of isolated rats. The ability of a compound to act as an antagonist or agonist for the ET _B receptor can be assessed by testing the compound's ability to inhibit endothelin-1 induced prostacyclin release from cultured bovine aortic endothelial cells.
[931] A. Endothelin Inhibition-Binding Test # 1: Inhibition of ET _A Receptor
[932] TE 671 cells (ATCC Accession No. HTB 139) express ET _A receptors. The cells are grown to confluence in T-175 flasks. Cells from multiple flasks are collected by scraping, pooled and centrifuged at 190 × g for 10 minutes. The cells are resuspended in 10 mM EDTA containing phosphate buffered saline (PBS) using a Tenbroeck homogenizer. The suspension is centrifuged at 57,800 × g for 15 minutes at 4 ° C., and the pellet is resuspended in 5 ml of Buffer A (pH 7.4 HEPES buffer at pH 7.4 with aprotinin (100KIU / ml)), then frozen once and thawed Let's do it. 5 ml of Buffer B (5 mM HEPES buffer at pH 7.4 containing 10 mM MnCl ₂ and 0.001% deoxyribonuclease type 1) is added, the suspension is mixed by inversion and then incubated at 37 ° C. for 30 minutes. The mixture was centrifuged at 57,800 × g as described above, the pellet washed twice with Buffer A and then resuspended in Buffer C (30 mM HEPES buffer at pH 7.4 containing aprotinin (100 Kiu / ml)) to 2 mg. A final protein concentration of / ml is obtained and then stored at -70 ° C until use.
[933] The membrane suspension is diluted to a concentration of 8 μg / 50 μl using binding buffer (30 mM HEPES buffer at pH 7.4 containing 150 mM NaCl, 5 mM MgCl ₂ , 0.5% Bacitracin). ^{Add 125} I-Endothelin-1 (3,000 cpm, 50 mL) to 50 μL of (A), (B) or (C): (A) Endothelin-1 (for nonspecific binding) (provides final concentration of 80 nM) ; (B) binding buffer (for total binding); Or (C) test compound (final concentration 1 nM to 100 μM). A membrane suspension (50 μL) containing about 8 μg of membrane protein is added to each of (A), (B) or (C). The mixture is shaken and incubated at 4 ° C. for 16-18 hours and then centrifuged at 4 ° C. for 25 minutes at 2,500 × g. In addition, the culture is carried out at 24 ℃. When incubated at 24 ° C., the IC ₅₀ concentration is 2 to 10 times higher than when incubated at 4 ° C. This fact should be kept in mind when comparing IC ₅₀ concentrations between the compounds provided herein.
[934] The supernatant containing unbound radioactivity is drained off and the pellet counted on a Genesys multiwell gamma counter. Binding inhibition rate (D) is calculated according to the following equation:
[935]
[936] Each test is generally performed three times.
[937] B. Endothelin Inhibition-Binding Test # 2: Inhibition of ET _B Receptor
[938] COS7 cells are transfected with DNA encoding the ET _B receptor. The resulting cells expressing human ET _B receptors are grown to confluence in T-150 flasks. Membranes are prepared as described above. Binding assays are performed as described above using membrane preparations diluted to a concentration of 1 μg / 50 μl with binding buffer.
[939] Briefly, stylized transfected with DNA for encoding the ET _B receptor, corn was grown in throw fluence COS7 a T-150 flask as described above, expressing the human ET _B receptor on the surface. Cells from multiple flasks are collected by scraping, pooled and centrifuged at 190 × g for 10 minutes. Cells are resuspended in 10 mM EDTA-containing phosphate buffered saline (PBS) using a Tenbroek homogenizer. The suspension is centrifuged at 57,800 × g for 15 minutes at 4 ° C., and the pellet is resuspended in 5 ml of Buffer A (pH 7.4 HEPES buffer at pH 7.4 containing aprotinin (100KIU / ml)), then frozen once and thawed Let's do it. 5 ml of Buffer B (5 mM HEPES buffer at pH 7.4 containing 10 mM MnCl ₂ and 0.001% deoxyribonuclease type 1) is added, the suspension is mixed by inversion and then incubated at 37 ° C. for 30 minutes. The mixture was centrifuged at 57,800 × g as described above, the pellet washed twice with Buffer A and then resuspended in Buffer C (30 mM HEPES buffer at pH 7.4 containing aprotinin (100 Kiu / ml)) to 2 mg. Obtain a final protein concentration of / ml.
[940] Binding assays are performed as described above using membrane preparations diluted to a concentration of 1 μg / 50 μl with binding buffer.
[941] C. Test for Activity on Endothelin-Induced Contraction of Thoracic Aortic Ring in Isolated Rats
[942] Efficacy of test compounds as antagonists or agonists of endothelin's biological tissue response can also be measured to determine the effect of endothelin-induced contraction of thoracic aortic ring in isolated rats [see, eg, Borges et al. (1989) Eur. J. Pharmacol. 165 : 223-230], assessed by measuring the ability of the tissue to contract when applied alone.
[943] The compound to be tested is prepared in 100 μM mother liquor. If dissolution needs to be performed, the compound is first dissolved in a minimum amount of DMSO and then diluted with 150 mM NaCl. Since DMSO can cause relaxation of the aortic annulus, control solutions containing different concentrations of DMSO are tested.
[944] The thoracic portion of the grown aorta is excised, gently rubbed off the endothelium and cut into 3 mm yun sections. The fragment was saturated with a gas mixture of 95% O ₂ and 5% CO ₂ (118 mM NaCl, 4.7 mM KCl, 1.2 mM MgSO ₄ , 1.2 mM KH ₂ PO ₄ , 25 mM NaHCO ₃ , 2.5 mM CaCl ₂ , Suspension under 2 g preload in a 10 ml tracheal bath filled with 10 mM D-glucose).
[945] There is a correlation between activity as an antagonist of endothelin-induced thoracic aortic constriction and activity as an inhibitor of endothelin binding to the endothelin receptor. pA ₂ is the linear logarithm function of IC ₅₀ .
[946] D. Assays to Identify Compounds Having Agonist and / or Antagonist Activity Against the ET _B Receptor
[947] 1. Stimulation of Prostacyclin Release
[948] Since endothelin-1 stimulates the release of prostacyclin from cultured bovine aortic endothelial cells, compounds with agonist or antagonist activity are substantially described in Filep et al . (1991) Biochem. Biophys. Res. Commun. 177 : 171-176, as determined by their ability to inhibit release of endothelin-1-induced prostacyclin from these endothelial cells by measuring 6-keto PGF _1α . Bovine aortic cells were inoculated into culture plates and grown in medium 199 supplemented with heat-inactivated 15% fetal bovine serum and L-glutamine (2 mM), penicillin, streptomycin and fungi and passaged four or more times. Obtained from the genease-treated bovine aorta. Cells are then seeded in 6-well plates in the same medium. 8 hours before the assay, cells reach confluence and medium is changed. The cells are then incubated with a) medium alone, b) medium containing endothelin-1 (10 nM), c) test compound alone, and d) test compound + endothelin-1 (10 nM).
[949] After 15 minutes of incubation, the medium is removed from each well and the concentration of 6-keto PGF _1α is measured by direct immunoassay. Prostacyclin production is calculated as the difference of the amount of 6-keto PGF _1α released by the cells challenged with endothelin-1 minus the amount released by the same untreated cells. 6-keto PGF _1α compound to stimulate the release has an agonist activity compounds that inhibit endothelin -1 6-keto PGF _1α release has an antagonist activity.
[950] 2. Suppression of Sarapotoxin 6c-induced Contraction
[951] Sarapotoxin 6c is a specific ET _B antagonist that constricts the bottom strip of rats. The efficacy of the test compound to inhibit the sarapotoxin 6c-induced contraction of the rat strip of rats is used as a measure of ET _B antagonist activity. Wedge strips of two separate rats were run in 10 μM cycles (D-Asp-Pro-D-Val-Leu-D-Trp) (BQ-123; see US Pat. No. 5,114,918, Ishikawa et al. ), 5 μM indometha Suspension under 1 g preload in a 10 ml engine bath filled with Krebs-Henzelite solution containing cine and saturated with a gas mixture of 95% O ₂ and 5% CO ₂ . The change in tension is measured isotropically and recorded using a Grass Polygraph coupled to the power converter. While Sarapotoxin 6c is added to the first strip with nodose, the second strip is preincubated with the test compound for 15 minutes prior to the addition of the incremental dose of Sarapotoxy 6c. Examine the effect of the test compound on the concentration-response curve for sarapotoxin 6c.
[952] E. Deoxycorticosterone acetate (DOCA) -salt hypertensive rat model for in vivo activity evaluation of selected compounds
[953] Selected compounds described herein are tested for activity in a deoxycorticosterone acetate (DOCA) -salt hypertensive rat model. To perform this test, a plastic MDX4-4210 elastomeric graft containing 47 mg (DOCA) was described in Ornmsbee et al. (1973) the J. Pharm. Sci. 62 : 255-257. Briefly, DOCA is incorporated into a silicone rubber graft for sustained release. To prepare the graft, DOCA is incorporated into the non-polymerized silicone rubber, the catalyst is added and the mixture is cast into a semicylindrical shape.
[954] Sprague Dolly rats (7-8 weeks old) are unilaterally resected under ketamine anesthesia and a DOCA-graft is placed on the left rear abdomen of the animal. Rats are allowed to recover for 3 weeks. During recovery, they provide free access to the 0.9% NaCl solution in place of normal rat food and drink. Rats develop symptoms of hypertension within three weeks.
[955] All animals are used for testing within 21-30 days after surgery. Average arterial blood pressure in the animal ranges from 165 to 200 mmHg.
[956] In the experiments, the catheter is inserted under the Brebital anesthesia into the right femoral aorta for blood pressure measurement and into the right femoral vein for administration of the selected compound. The animal is placed in a paper machine and restored until the mean blood pressure evenly recorded for 60 minutes. At this time, the selected compound or control vehicle is administered orally by intravenous or oral gavage with 60 minutes inhalation. Continue to record blood pressure for an additional 10 hours.
[957] F. Effect of Intravenous Administration on ET-1-Induced Blood Pressure Boosting Responses in Conscious, Autonomic Nerve Blocked Rats, a Model for Evaluating In Vivo Activity of Selected Compounds
[958] Male Sprague Dawley rats (250-450 g) are anesthetized (Brebital 50 mg / kg, IP) and cannula are inserted into the femoral artery for mean aortic pressure (MAP) measurement and into the femoral vein for intravenous drug administration. Place the animal in the paper machine to restore consciousness. Thirty minutes after autonomic nerve blockade (atropine methyl nitrate 3 mg / kg, IV, then propanol 2 mg / kg, IV) is administered. After 1 hour, the animals were intravenously injected with vehicle (0.5 ml), and after 30 minutes, ET-1 (control, 1 μg / kg) was administered intravenously. After recovering from the challenge, the test compound is administered by intravenous bolus administration (0.5 ml) and then ET-1 is challenged 30 minutes later. The results are expressed as the inhibition rate of the ET-1-induced blood pressure boosting response after administration of the test compound as compared to the blood pressure boosting response induced by the control ET-1 challenge. In some cases, the third ET-1 challenge is administered 90 minutes after the test compound is administered.
[959] G. Results
[960] 1. in vitro
[961] IC ₅₀ of each of the compounds of the above examples for the ET _A and ET _B receptors is measured. Almost all of the compounds have an IC ₅₀ of less than 10 μM for one or both of the ET _A and ET _B receptors. Many compounds have an IC ₅₀ of less than about 10 μM, others have an IC ₅₀ of less than about 1 μM and some compounds have an IC ₅₀ of less than about 0.1 μM. Many compounds are selective for the ET _A receptor because they have an IC ₅₀ for ET _A that is significantly lower (10-100 fold or more) than for the ET _B receptor. The remaining compound is ET _B selective.
[962] 2. In vivo
[963] a. Selected compounds, for example N- (4-chloro-3-methyl-5-isoxazolyl) -2- (N- (4-methylphenyl) aminocarbonyl) thiophen-3-sulfonamide, N- ( 4-bromo-3-methyl-5-isoxazolyl) -2- [3,4- (methylenedioxy) benzyl] benzo [b] thiophene-3-sulfonamide, N- (4-chloro-3 -Methyl-5-isoxazolyl) -2- (3,4-methylenedioxy) benzyl) benzo [b] thiophen-3-sulfonamide, N- (4-chloro-3-methyl-5-isox Zolyl) -2- [β-hydroxy (3,4-methylenedioxy) phenylethyl] thiophene-3-sulfonamide, and N- (4-chloro-3-methyl-5-isoxazolyl) -2 -(3,4-methylenedioxybenzylcarbonyl) thiophene-3-sulfonamide was tested in a model of hypertensive rats and was effective in lowering blood pressure.
[964] b. Selected compounds such as N- (4-chloro-3-methyl-5-isoxazolyl) -2-{[3,4- (methylenedioxy) phenyl] acetyl} thiophene-3-sulfonamide, N- (4-chloro-3-methyl-5-isoxazolyl) -2-{[2-acetyl-4,5- (methylenedioxy) phenyl] aminocarbonyl} thiophene-3-sulfonamide, N -(4-chloro-3-methyl-5-isoxazolyl) -2-[(4-methoxy-2-methylphenyl) aminocarbonyl] thiophene-3-sulfonamide, N- (4-chloro-3 -Methyl-5-isoxazolyl) -2- [cyano-4,5-dimethoxyphenyl) aminocarbonyl] thiophene-3-sulfonamide and N- (4-chloro-3-methyl-5-isoxe Sazolyl) -2- [2-methyl-4,5- (methylenedioxy) phenylacetyl] thiophene-3-sulfonamide was tested in autonomic neuron-blocked rat model of normal blood pressure and the blood pressure was 30 mg / It has been shown to have significant activity at about 30% lower in 30 minutes at doses as low as 50 kg and at 50% lower at 60 mg / kg. An average dosage of 30-60 mg / kg of test compound results in a 40-60% inhibition of the blood pressure boosting response.
[965] Since it is clear that modifications can be applied to those skilled in the art, the present invention is intended to be limited only by the scope of the appended claims.

权利要求:
Claims (12)
[1" claim-type="Currently amended] (a) dissolving free sulfonamide in an organic solvent;
(b) washing the dissolved free sulfonamide with a saturated solution of alkali metal salts; And
(c) recovering the alkali metal salt of sulfonamide.
[2" claim-type="Currently amended] The method of claim 1 wherein the organic solvent is ethyl acetate.
[3" claim-type="Currently amended] The method of claim 1 wherein the alkali metal is sodium, potassium, calcium or magnesium.
[4" claim-type="Currently amended] The method of claim 3 wherein the alkali metal is sodium.
[5" claim-type="Currently amended] The process of claim 4 wherein the saturated solution of alkali metal salts is saturated sodium bicarbonate or sodium carbonate.
[6" claim-type="Currently amended] The method of claim 5 wherein the saturated solution of alkali metal salts is saturated sodium bicarbonate.
[7" claim-type="Currently amended] The process of claim 1, wherein recovering (c) comprises drying the product of step (b), concentrating the dried product, crystallizing the concentrated product in one or more water-immiscible organic solvents, and Collecting the sulfonamide salt by filtration.
[8" claim-type="Currently amended] 8. The method of claim 7, wherein the water immiscible organic solvent is dichloromethane and ether.
[9" claim-type="Currently amended] The method of claim 1, wherein the free sulfonamide is 4-chloro-3-methyl-5- (2- (2- (6-methylbenzo [d] [1,3] dioxol-5-yl) acetyl) -3 Thienylsulfonamido) isoxazole; N ^2- (3-cyanomethyl-2,4,6-trimethylphenyl) -3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophencarboxamide, sodium salt ; N ^2- (3-acetyloxymethyl-2,4,6-trimethylphenyl) -3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophencarboxamide, sodium salt ; Or N ^2- (3-hydroxymethyl-2,4,6-trimethylphenyl) -3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophencarboxamide, sodium How salt is.
[10" claim-type="Currently amended] The free sulfonamide of claim 1, wherein (a) 5-chloromethyl-6-methylbenzo [d] [1,3] dioxole and activated magnesium are mixed in tetrahydrofuran to produce a Grignard reagent. Step (b) adding N ² -methoxy-N ² -methyl-3- (4-chloro-3-methyl-5-isoxazolylsulfamoyl) -2-thiophencarboxamide to the reaction mixture ; (c) diluting the mixture from step (b) in turn with a concentrated inorganic acid and an organic solvent to form an aqueous layer and an organic layer; And (d) drying the organic layer to form a residue containing the free acid. 4-chloro-3-methyl-5- (2- (2- (6-methylbenzo [d] [1, 3] dioxol-5-yl) acetyl) -3-thienylsulfonamido) isoxazole.
[11" claim-type="Currently amended] 4-Chloro-3-methyl-5- (2- (2- (6-methylbenzo [d] [1,3] dioxol-5-yl) acetyl) -3-thienyl prepared by the method of claim 1 Sulfonamido) alkali metal salts of isoxazoles.
[12" claim-type="Currently amended] 4-Chloro-3-methyl-5- (2- (2- (6-methylbenzo [d] [1,3] dioxol-5-yl) acetyl) -3-thienyl prepared by the method of claim 9 Sulfonamido) isoxazoles, sodium salts.

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同族专利:

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公开号 | 公开日

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CZ300859B6|2009-08-26|

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JP2008208143A|2008-09-11|

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US5783705A|1998-07-21|

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KR20010020362A|2001-03-15|

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PL200860B1|2009-02-27|

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EP1498419A1|2005-01-19|

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EP1498418A1|2005-01-19|

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KR100527620B1|2005-11-15|

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US6248767B1|2001-06-19|

---

KR100400795B1|2003-10-08|

---

EE200300214A|2003-08-15|

---

EP1498418B1|2008-05-21|

引用文献:

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公开号 | 申请日 | 公开日 | 申请人 | 专利标题

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法律状态:
1997-04-28|Priority to US08/847,797

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1997-04-28|Priority to US08/847,797

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1997-09-26|Priority to US08/938,444

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1997-09-26|Priority to US08/938,444

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1998-04-02|Application filed by 텍사스 바이오테크놀로지 코포레이션

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1998-04-02|Priority to PCT/US1998/006680

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2004-01-13|Publication of KR20040004368A

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2005-11-15|Application granted

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2005-11-15|Publication of KR100527620B1

优先权:

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申请号 | 申请日 | 专利标题

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US08/847,797|1997-04-28|

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US08/847,797|US5783705A|1997-04-28|1997-04-28|Process of preparing alkali metal salys of hydrophobic sulfonamides|

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US08/938,444|1997-09-26|

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US08/938,444|US6248767B1|1997-04-28|1997-09-26|Formulation of sulfonamides for treatment of endothelin-mediated disorders|

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PCT/US1998/006680|WO1998049162A1|1997-04-28|1998-04-02|Sulfonamides for treatment of endothelin-mediated disorders|